1887

Abstract

Sixteen heparinase-producing isolates, related to , were grouped into three major clusters by SDS-PAGE and DNA-rRNA . Based on a polyphasic approach, it was shown that isolates of two of these clusters and belong to a new genus for which the name is proposed. The genus consists of comb. nov. (formerly ), which is the type species, comb. nov. (formerly ), sp. nov. and sp. nov. and four as-yet-unnamed DNA hybridization groups. All the previously named taxa can be discriminated by phenotypic features, but have strong overall similarities with representatives of the genus and the misclassified species [. All these organisms constitute a separate rRNA branch in rRNA superfamily V for which the family fam. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-48-1-165
1998-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/48/1/ijs-48-1-165.html?itemId=/content/journal/ijsem/10.1099/00207713-48-1-165&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a gordian knot: emended classification and description of the genus Flavo- bacterium emended description of the family Flavobacteri- aceae and proposal of Flavobacterium hydatis nom. nov. (basonym Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148
    [Google Scholar]
  2. Christensen P. 1980a; Description and taxonomic status of Cytophaga heparina (Payza and Korn) comb. nov. (basi- onym: Flavobacterium heparinum Payza and Korn 1956). Int J Syst Bacteriol 30:473–475
    [Google Scholar]
  3. Christensen P. 1980b; Flexibacter canadensis sp. nov. Int J Syst Bacteriol 30:429–432
    [Google Scholar]
  4. Cowan S. T. 1979 Cowan Steel's Manual for the Identification of Medical Bacteria, 2nd. edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  5. Dees S. B., Carlone G., M„ Hollis D., Moss C. W. 1985; Chemical and phenotypic characteristics of Flavobacterium thalpophilum compared with those of other Flavobacterium and Sphingobacterium species. Int J Syst Bacteriol 35:16–22
    [Google Scholar]
  6. De Ley J. 1992; The Proteobacteria: ribosomal RNA cistron similarities and bacterial taxonomy. In The Prokaryotes, 2nd. edn, vol. 2 pp. 2111–2140 Balows A., Triiper H. G., Dworkin M., Harder W., Schleifer K.-H. Berlin: Springer;
    [Google Scholar]
  7. Dobson S. J., Colwell R. R., McMeekin T. A., Franzman P. D. 1993; Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gond- wanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline antarctic lake. Int J Syst Bacteriol 43:77–83
    [Google Scholar]
  8. Gherna R., Woese C. R. 1992; A partial phylogenetic analysis of the ‘ Flavobacter-Bacteroides' phylum: basis for taxonomic restructuring. Syst Appl Microbiol 15:513–51
    [Google Scholar]
  9. Hamana K., Matsuzaki S. 1991; Polyamine distributions in the Flavobacterium-Cytophaga-Sphingobacterium complex. Can J Microbiol 37:885–888
    [Google Scholar]
  10. Holmes B., Owen R. J., Hollis D. G. 1982; Flavobacterium spiritivorum a new species isolated from human clinical specimens. Int J Syst Bacteriol 32:157–165
    [Google Scholar]
  11. Holmes B., Owen R. i., Weaver R. E. 1981; Flavobacterium multivorum a new species isolated from human clinical specimens and previously known as group Ilk, biotype 2. Int J Syst Bacteriol 31:21–34
    [Google Scholar]
  12. Jacobi C. A., Reichenbach H., Tindall B. J., Stackebrandt E. 1996; Candidatus comitans, a bacterium living in coculture with Chondromyces crocatus (myxobacteria). Int J Syst Bacteriol 46:119–122
    [Google Scholar]
  13. Joubert J. J., van Rensburg E. J., Pitout M. J. 1984; A plate method for demonstrating the breakdown of heparin and chondroitin sulfate by bacteria. Microbiol Methods 2:197–202
    [Google Scholar]
  14. Joubert J. J. 1985 Kenmerking van heparinase pro-duserende mikro-organismes MD thesis, University of Pretoria
    [Google Scholar]
  15. Kersters K., Hinz K.-H., Hertle A., Segers P., Lievens A., Siegmann O., De Ley J. 1984; Bordetella avium sp. nov. isolated from the respiratory tracts of turkeys and other birds. Int J Syst Bacteriol 34:56–70
    [Google Scholar]
  16. Manz W., Amann R., Ludwig W., Vancanneyt M., Schleifer K.-H. 1996; Application of a suite of 16S rRNA- specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment. Microbiology 142:1097–1106
    [Google Scholar]
  17. Nakagawa Y., Yamasato K. 1993; Phylogenetic diversity of the genus Cytophaga revealed by 16S rRNA sequencing and menaquinone analysis. J Gen Microbiol 139:1155–1161
    [Google Scholar]
  18. Nakagawa Y., Yamasato K. 1996; Emendation of the genus Cytophaga and transfer of Cytophaga salmonicolor to Marinilabilia gen. nov.: phylogenetic analysis of the Flavo- bacterium-Cytophaga complex. Int J Syst Bacteriol 46:599–603
    [Google Scholar]
  19. Paster B. J., Ludwig W., Weisburg W. G., Stackebrandt E., Hespell R. B., Hahn C. M., Reichenbach H., Stetter K. O., Woese C. R. 1985; A phylogenetic grouping of the bacter- oides, cytophagas, and certain flavobacteria. Syst Appl Microbiol 6:34–42
    [Google Scholar]
  20. Payza A. N., Korn E. D. 1956; The degradation of heparin by bacterial enzymes. 1. Adaptation and lyophilized cells. J Biol Chem 223:853–864
    [Google Scholar]
  21. Reichenbach H. 1989a; Genus I. Cytophaga Winogradsky 1929, 577AL. Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. In Bergey's Manual of Systematic Bacteriology vol. 3 pp. 2015–2020 Baltimore: Williams Wilkins;
    [Google Scholar]
  22. Reichenbach H. 1989b; Order I. Cytophagales Leadbetter 1974, 99AL. Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. In Bergey's Manual of Systematic Bacteriology vol. 3 pp. 2011–2013 Baltimore: Williams Wilkins;
    [Google Scholar]
  23. Segers P., Mannheim W., Vancanneyt M., De Brandt K., Hinz K.-H., Kersters K., Vandamme P. 1993; Description of Riemerella anatipestifer gen nov., comb, nov., the causative agent of septicaemia anserum exsudativa and its phylogenetic affiliation within the Flavobacterium-Cyto- phaga rRNA hybridization group. Int J Syst Bacteriol 43:768–776
    [Google Scholar]
  24. Shivaji S., Ray M. K., Shyamale Rao N., Saisree L., Jagan- nadham M. V., Seshu Kumar G., Reddy G. S. N., Bhargava P. M. 1992; Sphingobacterium antarcticus sp. nov., a psychotrophic bacterium from soils of Schirmacher Oasis, Antarctica. Int J Syst Bacteriol 42:102–106
    [Google Scholar]
  25. Sonnenwirth A. C. 1980; Bacteriological methods. In GradwohFs Clinical Laboratory Methods and Diagnosis vol. 2, 8th. edn, pp. 1338–1377 Edited by Sonnenwirth A. C., Jarret W. London: C. V. Mosby;
    [Google Scholar]
  26. Steyn P. L., Pot B., Segers P., Kersters K., Joubert J. J. 1992; Some novel aerobic heparin-degrading bacteria. Syst Appl Microbiol 15:137–143
    [Google Scholar]
  27. Steyn P. L., Joubert J. J., Segers P., Pot B., Vancanneyt M., Willems A., Hoste B., Kersters K. 1993; The taxonomic position of some gram-negative aerobic heparinase producing bacteria. In Advances in the Taxonomy and Significance of Flavobacterium, Cytophaga and Related Bacteria pp. 137–151 Edited by Jooste P. J. Bloemfontein, South Africa: University of Orange Free State Press;
    [Google Scholar]
  28. Takeuchi M., Yokota A. 1992; Proposals of Sphingobacterium faecium sp. nov., Sphingobacterium piscium sp. nov., Sphingobacterium heparinum comb, nov., Sphingobacterium thalpophilum comb. nov. and two genospecies of the genus Sphingobacterium and synonymy of Flavobacterium yabuuchiae and Sphingobacterium spiritivorum. J Gen Appl Microbiol 38:465–482
    [Google Scholar]
  29. Vandamme P., De Ley J. 1992; Proposal for a new family, Campylobacteraceae. Int J Syst Bacteriol 41:451–455
    [Google Scholar]
  30. Vandamme P., Bernardet J.-F., Segers P., Kersters K., Holmes B. 1994; New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen nov. and Empedobacter nom. rev. Int J Syst Bacteriol 44:827–831
    [Google Scholar]
  31. Vannieuwenhuyze F., Sandra P. 1987; Selectivity opti- malization for the capillary gas chromatographic analysis of bacterial cellular fatty acids. Chromatographia 23:850855
    [Google Scholar]
  32. Yabuuchi E., Kaneko T., Yano I., Moss C. W., Miyoshi N. 1983; Sphingobacterium gen. nov., Sphingobacterium spiritivorum comb, nov., Sphingobacterium multivorum comb, nov., Sphingobacterium mizutae sp. nov., and Flavobacterium indologenes sp. nov.: glucose-nonfermenting gram-negative rods in CDC groups Ilk-2 and lib. Int J Syst Bacteriol 33:580–598
    [Google Scholar]
  33. Yabuuchi E., Moss C. W. 1982; Cellular fatty acid composition of strains of three species of Sphingobacterium gen. nov. and Cytophaga johnsonae. FEMS Microbiol Lett 13:87–91
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-48-1-165
Loading
/content/journal/ijsem/10.1099/00207713-48-1-165
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error