- International Journal of Systematic and Evolutionary Microbiology
- Volume 48, Issue 1
f Caldicellulosiruptor owensensis sp. nov., an anaerobic, extremely thermophilic, xylanolytic bacterium
- Authors: Chi-Yu Huang, Bharat K. Patel, Robert A. Mah, Larry Baresi
- Author for correspondence: Chi-Yu Huang. Tel: +1 310 825 2569. Fax: + 1 310 794 2106. e-mail: firstname.lastname@example.org
- Int J Syst Evol Microbiol, January 1998 48: 91-97, doi: 10.1099/00207713-48-1-91
- Subject: Systematic Bacteriology
- Published Online:
An anaerobic, extremely thermophilic xylanolytic, non-spore-forming bacterium was isolated from a sediment sample taken from Owens Lake, California, and designated strain OLT (T = type strain). Strain OLT had a Gram-negative reaction and occurred as short rods which sometimes formed long chains containing a few coccoid cells. It grew at 50--80 °C, with an optimum at 75 °C. The pH range for growth was 5·5--9·0 with an optimum at about pH 7·5. When grown on glucose at optimal conditions, its doubling time was 7·3 h. In addition to glucose, the isolate utilized sucrose, xylose, fructose, ribose, xylan, starch, pectin and cellulose. Yeast extract stimulated growth on carbohydrates but was not obligately required. The end products from glucose fermentation were lactate, acetate, ethanol, H2 and CO2. The G+C content of strain OLT was 36·6 mol%. The 16S rDNA sequence analysis indicated that strain OLT was a member of the subdivision containing Gram-positive bacteria with DNA G+C content of less than 55 mol% and clustered with members of the genus Caldicellulosiruptor. Because strain OLT is phylogenetically and phenotypically different from other members of this genus, it is proposed to designate this isolate Caldicellulosiruptor owensensis sp. nov. Strain OLT is the type strain (=ATCC700167T).
The GenBank accession number for the 16S rDNA sequence of strain OLT is U80596.
© 1998, International Union of Microbiological Societies | Published by the Microbiology Society
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