- International Journal of Systematic and Evolutionary Microbiology
- Volume 48, Issue 2
f Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov.
- Authors: Philippe de Lajudie, Anne Willems, Giselle Nick, Fatima Moreira, Flore Molouba, Bart Hoste, Urbain Torck, Marc Neyra, Matthew D. Collins, Kristina Lindström, Bernard Dreyfus†, Monique Gillis
- Author for correspondence: Monique Gillis. Tel: +32 9 264 5117. Fax: +32 9 264 5092. e-mail: Moniek.Gillis@rug.ac.be
- First Published Online: 01 April 1998, International Journal of Systematic and Evolutionary Microbiology 48: 369-382, doi: 10.1099/00207713-48-2-369
- Subject: Systematic Bacteriology
- Issue Published:
A collection of strains isolated from root nodules of Acacia species in Senegal was analysed previously by electrophoresis of total cell protein, auxanographic tests, rRNA-DNA hybridization, 16S rRNA gene sequencing, DNA base composition and DNA-DNA hybridization [de Lajudie, P., Willems, A., Pot, B. & 7 other authors (1994). Int J Syst Bacteriol 44, 715–733]. Strains from Acacia were shown to belong to two groups, Sinorhizobium terangae, and a so-called gel electrophoretic cluster U, which also included some reference strains from Brazil. Further taxonomic characterization of this group using the same techniques plus repetitive extragenic palindromic-PCR and nodulation tests is presented in this paper. Reference strains from Sudan and a number of new rhizobia isolated from nodules of Acacia Senegal, Acacia tortilis subsp. raddiana and Prosopis juliflora in Senegal were included. As a result of this polyphasic approach, the creation of a new species, Mesorhizobium plurifarium, is proposed for a genotypically and phenotypically distinct group corresponding to the former cluster U and containing strains isolated from Acacia, Leucaena, Prosopis and Chamaecrista in West Africa (Senegal), East Africa (Sudan) and South America (Brazil). The type strain of Mesorhizobium plurifarium ORS 1032 has been deposited in the LMG collection as LMG 11892.
The EMBL accession numbers for the new sequence data are Y14158 for M. plurifarium LMG 11892T, Y14161 for W. plurifarium LMG 10056, Y14159 for M. loti LMG 6123 and Y14160 for M. loti LMG 6124.
Present address: LSTM ORSTOM/CIRAD-Forêt, Baillarguet, BP 5035, 34032 Montpellier Cedex 1, France.
© 1998, International Union of Microbiological Societies | Published by the Microbiology Society
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