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Volume 48, Issue 2

Phylogenetic diversity of strains of various serotypes as revealed by 16S rRNA gene sequence comparison Free

Abstract

The 16S rRNA gene sequences of reference strains of suis serotypes 1–34 and 1/2 were determined. A comparative sequence analysis showed that the degree of sequence similarity between reference strains ranged from 93·94 to 100%. A dendrogram was constructed from the similarity matrix. Thirty-two strains representing 32 serotypes fell into a major group divided into three clusters. The other strains, serotypes 32, 33 and 34, were more distant. Biochemical characterization of the six more distant strains, including serotypes 20, 22, 26, 32, 33 and 34, revealed a profile similar to that of other serotypes. Comprison of the 16S rRNA gene sequences of reference strains with sequences of other members of the genus indicated that, with the exception of serotypes 32, 33 and 34, reference strains did not cluster with any other species in the genus. In conclusion, 16S rRNA gene sequence analysis defined a major group of reference strains which were very closely related and a higher divergence for serotypes 32, 33 and 34. However, to date, there is no strong evidence to reclassify strains of these serotypes in another species.

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Keyword(s): 16S rRNA gene sequence analysis and Streptococcus suis
© 1998, International Union of Microbiological Societies
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1998-04-01
2024-05-10
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References

  1. Arends J. P., Zanen H. C. 1988; Meningitis caused by Streptococcus suis in humans. Rev Infect Dis 10:131–137
     [Google Scholar]
  2. Beaudoin M., Harel J., Higgins R., Gottschalk M., Frenette M., Madnnes J. 1992; Molecular analysis of isolates of Streptococcus suis capsular type 2 by restriction-endonuclease-digested DNA separated on SDS-PAGE and by hybridization with an rDNA probe. J Gen Microbiol 138:2639–2645
     [Google Scholar]
  3. Bentley R. W., Leigh J. A., Collins M. D. 1991; Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences. Int J Syst Bacteriol 41:487–494
     [Google Scholar]
  4. Clifton-Hadley F. A. 1983; Streptococcus suis type 2 infections. Br Vet J 139:1–5
     [Google Scholar]
  5. Christensen J. J., Whitney A. M., Teixeira L. M., Steigerwalt A. G., Facklam R. R., Korner B., Brenner D. J. 1997; Aerococcus urinae·. intraspecies genetic and phenotypic relatedness. Int J Syst Bacteriol 47:28–32
     [Google Scholar]
  6. Devereux J., Haeberli P., Smithies D. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395
     [Google Scholar]
  7. Devriese L A., Haesebrouck F., de Herdt P., Dom P., Ducatelle R., Desmidt M., Messier S., Higgins R. 1994; Streptococcus suis infections in birds. Avian Pathol 23:721–724
     [Google Scholar]
  8. Dorch M., Stackebrandt E. 1992; Some modifications in the procedure of direct sequencing of PCR amplified 16S rDNA. J Microbiol Methods 16:271–279
     [Google Scholar]
  9. Felsenstein J. 1995; phylip - Phylogeny Inference Package (version 3.51c). University of Washington; Seattle:
     [Google Scholar]
  10. Galina L, Collins J. E., Pijoan C. 1992; Porcine Streptococcus suis in Minnesota. J Vet Diagn Invest 4:195–196
     [Google Scholar]
  11. Gottschalk M., Higgins R., Jacques M., Mittal K. R., Henrichsen J. 1989; Description of 14 new capsular types of Streptococcus suis. J Clin Microbiol 27:2633–2635
     [Google Scholar]
  12. Gottschalk M., Higgins R., Jacques M., Beaudoin M., Henrichsen J. 1991; Characterization of six new capsular types (23 through 28) of Streptococcus suis. J Clin Microbiol 29:2590–2594
     [Google Scholar]
  13. Gottschalk M., Higgins R., Boudreau M. 1993; Use of polyvalent coagglutination reagents for serotyping of Streptococcus suis. J Clin Microbiol 31:2192–2194
     [Google Scholar]
  14. Hampson D. J., Trott D. J., Clarke L. T., Mwaniki C. G., Roberstson I. D. 1993; Population structure of Australian isolates of Streptococcus suis. J Clin Microbiol 31:2895–2900
     [Google Scholar]
  15. Harel J., Higgins R., Gottschalk M., Bigras-Poulin M. 1994; Genomic relatedness among reference strains of different Streptococcus suis serotypes. Can J Vet Res 58:259–262
     [Google Scholar]
  16. Higgins D., Bleasby A., Fuchs R. 1992; clustal v: improved software for multiple sequence analysis. Comput Appl BiosciS188–192
     [Google Scholar]
  17. Higgins R., Gottschalk M., Boudreau M., Lebrun A., Henrichsen J. 1995; Description of six new capsular types (29-34) of Streptococcus suis. J Vet Diagn Invest 7:405–406
     [Google Scholar]
  18. Higgins R., Gottschalk M. 1996; Distribution of Streptococcus suis capsular types in 1995. J Vet Diagn Invest 2:249–252
     [Google Scholar]
  19. Hogg A., Amass S. F., Hoffman L. J., Wu C. C., Clark L. K. 1996; A survey of Streptococcus suis isolations by serotype and tissue of origin. Proc Am Assoc Swine Pract79–80
     [Google Scholar]
  20. Intelligenetics Inc 1996; GeneWorks program (version 2.5). Mountain View; USA:
     [Google Scholar]
  21. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. Mammalian Protein Metabolism 321–132 Edited by Munro H. N. New York: Academic Press;
     [Google Scholar]
  22. Kilpper-Bälz R., Schleifer K. H. 1987; Streptococcus suis sp. nov., nom. rev. Int J Syst Bacteriol 37:160–162
     [Google Scholar]
  23. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
     [Google Scholar]
  24. Mogollon J. D., Pijoan C., Murtaugh M. P., Kaplan E. L., Collins J. E., Cleary P. P. 1990; Characterization of prototype and clinically defined strains of Streptococcus suis by genomic fingerprinting. J Clin Microbiol 28:2462–2466
     [Google Scholar]
  25. Okwumabua O., Staats J., Chengappa M. M. 1995; Detection of genomic heterogeneity in Streptococcus suis isolates by DNA restriction fragment length polymorphisms of rRNA genes (ribotyping). J Clin Microbiol 33:968–972
     [Google Scholar]
  26. Perch B., Pedersen K. B., Henrichsen J. 1983; Serology of capsulated streptococci pathogenic for pigs: six new serotypes of Streptococcus suis. J Clin Microbiol 17:993–996
     [Google Scholar]
  27. Pitcher D. G., Saunders N. A., Owen R. J. 1989; Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8:151–156
     [Google Scholar]
  28. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
     [Google Scholar]
  29. Smith H. E., Rijnsburger M., Stockhofe-Zurwieden N., Wisselink H. J., Vecht U., Smits M. A. 1997; Virulent strains of Streptococcus suis serotype 2 and highly virulent strains of Streptococcus suis serotype 1 can be recognized by a unique ribotype profile. J Clin Microbiol 35:1049–1053
     [Google Scholar]
  30. Stackebrandt E., Goebel M. 1994; Taxonomic note: place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
     [Google Scholar]
  31. Taghavi M., Hayward C., Sly L. I., Fegan M. 1996; Analysis of the phylogenetic relationship of strains of Burkholderia solanacearum, Pseudomonas syzygii, and the blood disease bacterium of banana based on 16SrRNA gene sequences. Int J Syst Bacteriol 46:10–15
     [Google Scholar]
  32. Tarradas C., Arenas A., Maldonado A., Luque I., Miranda A., Perea A. 1994; Identification of Streptococcus suis isolated from swine: proposal for biochemical parameters. J Clin Microbiol 32:578–580
     [Google Scholar]
  33. Trottier S., Higgins R., Brochu G., Gottschalk M. 1991; A case of human endocarditis due to Streptococcus suis in North America. Rev Infect Dis 13:1251–1252
     [Google Scholar]
  34. Vetch U., van Leengoed M. G., Verheyen E. R. M. 1985; Streptococcus suis infections in pigs in the Netherlands (part one). Vet QI315–321
     [Google Scholar]
  35. Williams A. M., Collins M. D. 1990; Molecular taxonomic studies on Streptococcus uberis types I and II. Description of Streptococcus parauberis sp. nov. J Appl Bacteriol 68:485–490
     [Google Scholar]
  36. Woese C. R. 1987; Bacterial evolution. Microbiol Rev 51:221–271
     [Google Scholar]
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Phylogenetic diversity of Streptococcus suis strains of various serotypes as revealed by 16S rRNA gene sequence comparison
Int J Syst Evol Microbiol 48, 581 (1998); https://doi.org/10.1099/00207713-48-2-581
/content/journal/ijsem/10.1099/00207713-48-2-581
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