RT Journal Article SR Electronic(1) A1 Lien, Torleiv A1 Madsen, Marit A1 Rainey, Fred A. A1 Birkeland, Nils-KåreYR 1998 T1 Petrotoga mobilis sp. nov., from a North Sea oil-production well JF International Journal of Systematic and Evolutionary Microbiology, VO 48 IS 3 SP 1007 OP 1013 DO https://doi.org/10.1099/00207713-48-3-1007 PB Microbiology Society, SN 1466-5034, AB Rod-shaped, thermophilic bacteria with a sheath-like outer structure (‘toga’) were isolated from hot oilfield water of a North Sea oil reservoir. One of the isolates, designated SJ95T, is an obligately anaerobic, sheathed, Gram-negative, fermentative bacterium capable of reducing elemental sulfur to hydrogen sulfide and tolerating high salt concentrations. The optimum growth conditions for this isolate are 58-60°C and pH 6.5-7.0 with 3-4% NaCl and 0.7% MgSO4.7H2O in the medium. Vitamins are required for growth. Growth is stimulated by yeast extract. Cells of strain SJ95Tvary in size from 1-2 to 40-50 μm in length and are motile with a subpolar flagellation. Cells grown on xylan have xylanase activity, presumably associated with the toga, and glucose isomerase activity was detected in xylose-grown cells. The DNA G+C content is 31 and 34 mol%, determined by the thermal denaturation and HPLC methods, respectively. Phylogenetically, strain SJ95Tis most closely related to Petrotoga miotherma with a 97.7% similarity level between their 16S rDNA sequences. The DNA-DNA reassociation value between the two DNAs was 35.6%. On the basis of differences in genotypic, phenotypic and immunological characteristics, strain SJ95T(= DSM 10674T) is proposed as the type strain of a new species, Petrotoga mobilis. It can be readily distinguished from P. miotherma by its motility., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-48-3-1007