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Volume 48, Issue 3

sp. nov., a new species that is enteropathogenic for chickens Free

Abstract

Strain C1is an anaerobic spirochaete that causes intestinal disease in chickens. Multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons have indicated that this spirochaete is a strain. In these investigations, various phenotypic and genomic properties useful for establishing a taxonomic identity for strain C1were studied. As determined by electron microscopy, cells of the spirochaete measured 8-11 x 0·22-0·34 µm and had a typical spirochaete ultrastructure. Each cell had 22·30 flagella. C1cells formed weakly β-haemolytic colonies on trypticase soy agar plates containing 5% bovine blood. The spirochaete reached maximum population densities of 10cells mlwith a 2·4 h population doubling time in brain heart infusion broth containing 10% calf serum (BHIS broth). C1cultures in BHIS broth were positive in tests for hippurate hydrolysis and negative for indole production. Glucosamine, , glucose, fructose, maltose and mannose were growth substrates for the spirochaete in heart infusion broth containing 7% calf serum (HS broth). During growth in HS broth beneath an O/N (1:99) atmosphere, cells of the spirochaete consumed O and glucose and produced H, CO, acetate, butyrate and ethanol. Strain C1DNA had a G+C content of 24·6 mol%. Based on DNA-DNA hybridization analyses, the DNA of strain C1exhibited 24·39% relative reassociation with DNA of and These results indicate that chicken spirochaete strain C1has many phenotypic properties common to species and, based on DNA hybridization analysis, represents a unique species. For this new species the name is proposed, for which the type strain is C1(= ATCC 51933).

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Keyword(s): chickens , enteropathogenic bacteria , Serpulina alvinipulli sp. nov. and spirochaetes
© Society for General Microbiology, 1998
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1998-07-01
2024-04-18
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Serpulina alvinipulli sp. nov., a new Serpulina species that is enteropathogenic for chickens
Int J Syst Evol Microbiol 48, 669 (1998); https://doi.org/10.1099/00207713-48-3-669
/content/journal/ijsem/10.1099/00207713-48-3-669
/content/journal/ijsem/10.1099/00207713-48-3-669
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