1887

Abstract

The species was proposed to accommodate a heterogeneous group of streptococci associated with infections in animals and human beings. This taxon is now considered to include animal isolates of -haemolytic group C streptococci, previously called . ; animal and human isolates of -haemolytic group C streptococci, previously called ‘. ’; -haemolytic group L strains associated with infections in animals and, rarely, in humans; and -hiaemolytic group G strains isolated from humans. DNA-DNA reassociation experiments (hydroxyapatite method) and multilocus enzyme electrophoresis (MEE) were performed on reference strains and clinical isolates to determine the genetic relationships among these different phenotypic categories. DNA-DNA hybridization tests showed that they were related at the species level, despite the phenotypic and host heterogeneity. Both genotypic and phenotypic characterization indicated that . could be separated into two major sub-groups. The first subgroup contained -haemolytic strains that showed levels of DNA relatedness with the type strain of . ranging from 84 to 90% and from 82 to 88% under optimal (55 °C) and stringent (70 °C) conditions, respectively. The second sub-group contained haemolytic strains showing levels of relatedness ranging from 71 to 79% (55 °C) and from 62 to 73% (70 °C). Percentage divergence varied from 0·5 to 1·0% (-haemolytic group) and from 20 to 3·5% (-haemolytic group). A dendrogram based on phenotypic similarity between the enzyme bands produced by MEE showed a Jaccard similarity coefficient of 0·45 between the subclusters formed by the two sub-groups. The results of phenotypic and genotypic characterization were consistent with a published proposal to divide . into two subspecies, . subsp. and . subsp. , with a few modifications.

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1998-10-01
2024-03-29
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