1887

Abstract

Thirteen Gram-negative bacterial isolates were recovered from diseased pigeons and were tentatively classified as -like strains based on conventional phenotypic features and disease symptoms. Phenotypic characteristics that differentiated the pigeon isolates from included their greyish-white to beige pigment formation on Columbia blood agar and the hydrolysis of aesculin. Furthermore, strains have thus far not been reported in pigeons. The phenotypic differences together with the unique host range of the new isolates have prompted the inclusion of these strains in a polyphasic taxonomic study. Extensive phenotypic examination, PAGE of total proteins and GC analysis of fatty acid contents revealed that the pigeon isolates constitute a homogeneous cluster, distinct from the reference strains. The phylogenetic position of representative strains was examined by using DNA–rRNA hybridizations and indicated that this taxon belongs to the genus . Finally, DNA-binding values confirmed that the strains constitute a separate species for which the name sp. nov. is proposed. Strain LMG 11607 was selected as the type strain. Clinical observations suggest that these organisms are involved in pathogenesis of respiratory diseases, similar to those associated with infections. However, the role of co-factors and the interaction with other agents are unknown.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-1-289
1999-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/1/ijs-49-1-289.html?itemId=/content/journal/ijsem/10.1099/00207713-49-1-289&mimeType=html&fmt=ahah

References

  1. Bernardet J.-F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. 1996; Cutting a Gordian knot: emended classification and description of the genus Flavobacterium emended description of the family Flavobacteriaceae and proposal of Flavobacterium hydatis nom. nov. (basonym Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46:128–148
    [Google Scholar]
  2. Bøvre K. 1984; Genus II. Moraxella Lwoff 1939, 173 emend. Henriksen and Bøvre 1968, 391AL. In Bergey’s Manual of Systematic Bacteriology 1296–303 Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Brodgen K. A. 1989; Pasteurella anatipestifer infection. In Pasteurella and Pasteurellosis115–129 Adlam C., Rutter J. M. London: Academic Press;
    [Google Scholar]
  4. De Ley J. 1970; Re-examination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101:738–754
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142
    [Google Scholar]
  6. Hinz K.-H., Ryll M., Köhler B. 1998a; Detection of acid production from carbohydrates by Riemerella anatipestifer and related organisms using the buffered single substrate test. Vet Microbiol (in press)
    [Google Scholar]
  7. Hinz K.-H., Ryll M., Köhler B., Glünder G. 1998b; Phenotypic characteristics of Riemerella anatipestifer and similar microorganisms from various hosts. Avian Pathol 27:33–42
    [Google Scholar]
  8. Lautrop H. 1960; Laboratory diagnosis of whooping cough or Bordetella infections. Bull WHO 23:15–31
    [Google Scholar]
  9. Mannheim W. 1984; Family III. Pasteurellaceae POHL 1981a, 382.vp. In Bergey’s Manual of Systematic Bacteriology 1550–557 Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  10. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118
    [Google Scholar]
  11. Piechulla K., Pohl S., Mannheim W. 1986; Phenotypic and genetic relationships of so-called Moraxella (Pasteurella) anatipestifer to the Flavobacterium/Cytophaga group. Vet Microbiol 11:261–270
    [Google Scholar]
  12. Pot B., Vandamme P., Kersters K. 1994; Analysis of electrophoretic whole-organism protein fingerprints. In Chemical Methods in Prokaryotic Systematics493–521 Goodfellow M., O’Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  13. Riemer. 1904; Kurze Mitteilung über eine bei Gänsen beobachtete exsudati ve Septikämie und deren Erreger. Zentbl Bakteriol I Abt Orig 37:641–648
    [Google Scholar]
  14. Rossau R., Van Landschoot A., Gillis M., De Ley J. 1991; Taxonomy of Moraxellaceae fam. nov., a new bacterial family to accommodate the genera Moraxella Acinetobacter and Psychrobacter and related organisms. Int J Syst Bacteriol 41:310–319
    [Google Scholar]
  15. Segers P., Mannheim W., Vancanneyt M., De Brandt K., Hinz K.-H., Kersters K., Vandamme P. 1993; Riemerella anatipestifer gen. nov., comb. nov., the causative agent of septicemia anserum exsudativa, and its phylogenetic affiliation within the Flavobacterium-Cytophaga rRNA homology group. Int J Syst Bacteriol 43:768–776
    [Google Scholar]
  16. Subramaniam S., Chua K.-L., Tan H.-M., Loh H., Kuhnert P., Frey J. 1997; Phylogenetic position of Riemerella anatipestifer based on 16S rRNA gene sequences. Int J Syst Bacteriol 47:562–565
    [Google Scholar]
  17. Vandamme P., Vancanneyt M., Pot B. 10 other authors 1992; Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42:344–356
    [Google Scholar]
  18. Vandamme P., Segers P., Ryll M. 8 other authors 1998; Pelistega europaea gen. nov., sp. nov., a bacterium associated with respiratory disease in pigeons: taxonomic structure and phylogenetic allocation. Int J Syst Bacteriol 48:431–440
    [Google Scholar]
  19. Van Landschoot A., De Ley J. 1983; Intra- and intergeneric similarities of the rRNA cistrons of Alter omonas Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129:3057–3074
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-1-289
Loading
/content/journal/ijsem/10.1099/00207713-49-1-289
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error