- International Journal of Systematic and Evolutionary Microbiology
- Volume 49, Issue 1
f Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb, nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov.
- Authors: Øystein Angen†, Reinier Mutters, Dominique A. Caugant, John E. Olsen, Magne Bisgaard
- Author for correspondence: Øystein Angen. Fax: +45 35300120. e-mail: firstname.lastname@example.org
- First Published Online: 01 January 1999, International Journal of Systematic and Evolutionary Microbiology 49: 67-86, doi: 10.1099/00207713-49-1-67
- Subject: New Taxa - Proteobacteria
- Issue Published:
Taxonomic relationships of the [Pasteurella] haemolytica complex as evaluated by DNA-DNA hybridizations and 16S rRNA sequencing with proposal of Mannheimia haemolytica gen. nov., comb, nov., Mannheimia granulomatis comb. nov., Mannheimia glucosida sp. nov., Mannheimia ruminalis sp. nov. and Mannheimia varigena sp. nov., Page 1 of 1< Previous page | Next page > /docserver/preview/fulltext/ijsem/49/1/ijs-49-1-67-1.gif
The present paper presents the conclusions of a polyphasic investigation of the taxonomy of the trehalose-negative [Pasteurella] Haemolytica complex. Clusters previously identified by ribotyping and multilocus enzyme electrophoresis (MEE) have been evaluated by 16S rRNA sequencing and DNA-DNA hybridizations. Results obtained by the different techniques were highly related and indicated that the [P.] haemolytica complex contains distinct genetic and phenotypic groups. At least seven species were outlined, five of which were named. We refrained in formal naming of more groups until additional strains are characterized. Five 16S rRNA clusters were identified corresponding to distinct lineages previously outlined by MEE. Within 16S rRNA cluster I two distinct genotypic groups have been outlined in addition to [P.] haemolytica sensu stricto (biogroup 1). Each of the clusters II, III, IV and V represent at least one new species. The investigations underline that [P.] haemolytica sensu stricto only contains strains that do not ferment L-arabinose even though they are referred to as ‘biotype A’ of [P.] haemolytica. The five 16S rRNA clusters identified had a common root relative to the other species within the family Pasteurellaceae, and the overall sequence similarity among these five clusters was higher than what is observed within the existing genera of the family. The allocation of the trehalose-negative [P.] haemolytica complex to a new genus seems to be indicated. Based on the polyphasic investigation performed a new genus Mannheimia is proposed for the trehalose-negative [P.] haemolytica complex. At the present stage two previously named species are transferred to this new genus and three new species are described. [P.] haemolytica is reclassified as Mannheimia haemolytica comb. nov., whereas Pasteurella granulomatis, Bisgaard taxon 20 and [P.] haemolytica biovar 3J are reclassified and combined in the species Mannheimia granulomatis comb. nov. Mannheimia glucosida sp. nov.
The GenBank accession numbers for the sequences reported in this paper are listed in Table 1.
Present address: Danish Veterinary Laboratory, Department of Microbiology, Bülowsvej 27, DK-1790 Copenhagen V, Denmark.
- Keyword(s): 16s rRNA sequencing, polyphasic taxonomy, Mannheimia gen. nov., Pasteurella haemolytica, DNA-DNA hybridization
© 1999, International Union of Microbiological Societies | Published by the Microbiology Society
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