- International Journal of Systematic and Evolutionary Microbiology
- Volume 49, Issue 2
f Williamsia muralis gen. nov., sp. nov., isolated from the indoor environment of a children's day care centre
- Authors: Peter Kämpfer, Maria A. Andersson, Fred A. Rainey, Reiner M. Kroppenstedt, Mirja Salkinoja-Salonen
- Author for correspondence: Peter Kämpfer. Tel: +49 641 99 37352. Fax: +49 641 99 37359. e-mail: firstname.lastname@example.org
- Int J Syst Evol Microbiol, April 1999 49: 681-687, doi: 10.1099/00207713-49-2-681
- Subject: New Taxa - Gram-Positive Bacteria
- Published Online:
The taxonomic status of an actinomycete (MA140/96T) isolated from indoor building materials of a children's day care centre was studied using the polyphasic approach. The cell morphology was atypical for an actinomycete, electron microscopy revealed a hairy surface, highly unusual for Gram-positi bacteria. The organisms grew at 10--37°C no growth was visible at 5 °C and 45 °C in 5 d. The cell wall contained the diamino acid meso-diaminopimelic acid and the sugars arabinose, galactose, mannose and ribose. The phospholipids phosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol were detected. The only menaquinone found was MK9(H2). The fatty acid pattern was composed of palmitic acid (23.6%) palmitole acid (16.5%) and another hexadecenoic acid 16:1cis11 (1.4%), oleic acid (29.9%), stearic acid (2.9%) and the 10-methyl-branched tuberculostearic acid (23.2%). A gas-chromatographic analysis of the mycolic acid revealed a carbo chain length of C50-C56The G+C was 64.8 mol%. The results of 16S rDNA sequence comparisons revealed that strain MA140/96Trepresents a new lineage in the suborder Corynebacterineae of the order Actinomycetales. Therefore, it was concluded that strain MA140/96Tshould be assigned to a new genus and species, for which the name Williamsia muralis gen. nov., sp. nov. proposed. The type strain of the species is MA140/96T(= DSM 44343T).
The EMBL accession number for the 16S rDNA sequence of strain MA140/96Tis Y17384.
© 1999, International Union of Microbiological Societies | Published by the Microbiology Society
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