@article{mbs:/content/journal/ijsem/10.1099/00207713-49-2-681, author = "Kämpfer, Peter and Andersson, Maria A. and Rainey, Fred A. and Kroppenstedt, Reiner M. and Salkinoja-Salonen, Mirja", title = "Williamsia muralis gen. nov., sp. nov., isolated from the indoor environment of a children’s day care centre", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "1999", volume = "49", number = "2", pages = "681-687", doi = "https://doi.org/10.1099/00207713-49-2-681", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-2-681", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "actinomycete", keywords = "Williamsia muralis gen. nov.", keywords = "sp. nov.", keywords = "indoor contaminant", abstract = "The taxonomic status of an actinomycete (MA140/96T) isolated from indoor building materials of a children’s day care centre was studied using the polyphasic approach. The cell morphology was atypical for an actinomycete, electron microscopy revealed a hairy surface, highly unusual for Gram-positive bacteria. The organisms grew at 10–37 °C, no growth was visible at 5 °C and 45 °C in 5 d. The cell wall contained the diamino acid meso-diaminopimelic acid and the sugars arabinose, galactose, mannose and ribose. The phospholipids phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol were detected. The only menaquinone found was MK-9(H2). The fatty acid pattern was composed of palmitic acid (23·6%) palmitoleic acid (16·5%) and another hexadecenoic acid 16:1cis11 (1·4%), oleic acid (29·9%), stearic acid (2·9%) and the 10-methyl-branched tuberculostearic acid (23·2%). A gas-chromatographic analysis of the mycolic acid revealed a carbon-chain length of C50–C56. The G+C was 64·8 mol%. The results of 16S rDNA sequence comparisons revealed that strain MA140/96T represents a new lineage in the suborder Corynebacterineae of the order Actinomycetales. Therefore, it was concluded that strain MA140/96T should be assigned to a new genus and species, for which the name Williamsia muralis gen. nov., sp. nov. is proposed. The type strain of the species is MA140/96T (= DSM 44343T).", }