- International Journal of Systematic and Evolutionary Microbiology
- Volume 49, Issue 2
f Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system
- Authors: Sibylle Kalmbach, Werner Manz, Jörg Wecke, Ulrich Szewzyk
- Author for correspondence: Werner Manz. Tel: +49 30 314 25589. Fax: +49 30 314 73461. e-mail: email@example.com
- Int J Syst Evol Microbiol, April 1999 49: 769-777, doi: 10.1099/00207713-49-2-769
- Subject: New Taxa - Proteobacteria
- Published Online:
Aquabacterium gen. nov., with description of Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov., three in situ dominant bacterial species from the Berlin drinking water system, Page 1 of 1< Previous page | Next page > /docserver/preview/fulltext/ijsem/49/2/ijs-49-2-769-1.gif
Three bacterial strains isolated from biofilms of the Berlin drinking water system were characterized with respect to their morphological and physiological properties and their taxonomic position. Phenotypically, the bacteria investigated were motile, Gram-negative rods, oxidase-positive and catalase-negative, and contained polyalkanoates and polyphosphate as storage polymers. They displayed a microaerophilic growth behaviour and used oxygen and nitrate as electron acceptors, but not nitrite, chlorate, sulfale or ferric iron. The substrates metabolized included a broad range of organic acids but no carbohydrates at all. The three species can be distinguished from each other by their substrate utilization, ability to hydrolyse urea and casein, cellular protein patterns and growth on nutrient-rich media as well as their temperature, pH and NaCI tolerances. Phylogenetic analysis, based on 16S rRNA gene sequence comparison, revealed that the isolates are affiliated to the β1-subclass of Proteobacteria. The isolates constitute three new species with internal levels of DNA relatedness ranging from 44--9 to 51--3%. It is proposed that a new genus, Aquabacterium gen. nov., should be created, including Aquabacterium citratiphilum sp. nov., Aquabacterium parvum sp. nov. and Aquabacterium commune sp. nov. The type species of the new genus is Aquabacterium commune. The type strain of A. citratiphilum is strain B4T(= DSM 11900T), the type strain of A, parvum is strain B6T(= DSM 11968Tand the type strain of A. commune is strain B8T(= DSM 11901T).
The GenBank accession numbers for the 16S rRNA sequences reported in this paper are AF035047--AF035054 (strains B1--B8, respectively).
© 1999, International Union of Microbiological Societies | Published by the Microbiology Society
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