RT Journal Article SR Electronic(1) A1 Scholten, Edzard A1 Lukow, Thomas A1 Auling, Georg A1 Kroppenstedt, Reiner M. A1 Rainey, Fred A. A1 Diekmann, HansYR 1999 T1 Thauera mechernichensis sp. nov., an aerobic denitrifier from a leachate treatment plant JF International Journal of Systematic and Evolutionary Microbiology, VO 49 IS 3 SP 1045 OP 1051 DO https://doi.org/10.1099/00207713-49-3-1045 PB Microbiology Society, SN 1466-5034, AB A heterotrophic bacterial strain TL1T capable of aerobic denitrification was previously enriched in continuous culture from a landfill leachate treatment plant and isolated as a pure culture. The taxonomic position of this isolate within the β-subclass of the Proteobacteria was determined by 16S rDNA sequence analysis and by conventional taxonomy including substrate spectrum, quinone type (ubiquinone Q-8) and cellular fatty acid composition. Detection of the specific polyamine 2-hydroxyputrescine supports the membership of strain TL1T in the β-subclass of the Proteobacteria. The results of 16S rDNA sequencing showed that the strain clustered with, but was separate from, Thauera aromatica and Thauera selenatis. DNA-DNA hybridization experiments indicated that the new isolate represents a new species of the genus, for which the name Thauera mechernichensis is proposed; the type strain is DSM 12266T., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-49-3-1045