1887

Abstract

The almost complete sequence of the 16S rRNA gene of the Gram-positive polysporogenic bacterium was determined. This allowed phylogenetic analysis of by comparing sequences of the 16S rRNA gene of this bacterium to similar genes of other Gram-positive bacteria. It was shown that this polysporogenic bacterium belongs to the cluster I, subcluster A. Phylogenetically, is distantly related to another polysporogenic, but non-cultivatable, bacterium, ‘’ and can be satisfactorily clustered within the saccharolytic clostridia with a low DNA G+C content grouped in subcluster A. was most closely related to (94.8% identity of 16S rRNA genes) and (93.1 %). Like other members of the cluster I, subcluster A, possesses such common phenotypic features as a Gram-positive cell wall structure, anaerobiosis, derivation of energy from carbohydrate fermentation yielding butyric acid among other organic acids and the capacity for endogenous spore-formation. However, the scale of evolutionary change in the 16S rRNA gene between and phylogenetically related species does not correspond to the profound changes in the phenotype of Distinctive phenotypic features of the latter are large cell size, polysporogenesis (up to seven spores per cell), alternative modes of development and an unusual membrane ultrastructure.

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1999-07-01
2024-03-28
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References

  1. Angert E. R., Brooks A. E., Pace N. R. 1996; Phylogenetic analysis of Metabacterium polyspora: clues to the evolutionary origin of daughter cell production in Epulopiscium species, the largest bacteria. J Bacteriol 178:1451–1456
    [Google Scholar]
  2. Chumakov K. M. 1987; Evolution of nucleotide sequences. Soviet Sci Rev 8:215–264
    [Google Scholar]
  3. Collins M. D., Lawson P. A., Willems A., Cordoba J. J., Fernandez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826
    [Google Scholar]
  4. Duda V. I. 1982; Peculiarities of cytology of spore-forming bacteria. Uspekhi Mikrobiologii 17:87–117 (in Russian)
    [Google Scholar]
  5. Duda V. I., Makaryeva E. D. 1977; Morphogenesis and function of gas caps on spores of anaerobic bacteria belonging to the genus Clostridium. Mikrobiologiya 46:689–694 (in Russian)
    [Google Scholar]
  6. Duda V. I., Mushegjan M. S., Lebedinsky A. V., Mitjushina L. L. 1985; Formation of four-five endospores per cell by a new anaerobic bacterium. Dokl Akad Nauk SSSR 285:241–245 (in Russian)
    [Google Scholar]
  7. Duda V. I., Lebedinsky A. V., Mushegjan M. S., Mitjushina L. L. 1987; A new anaerobic bacterium, forming up to five endospores per cell - Anaerobacter polyendosporus gen. et spec, nov. Arch Microbiol 148:121–127
    [Google Scholar]
  8. Edwards U., Rogall T., Blocker H., Emde M., Bottger E. C. 1989; Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res 17:7843–7853
    [Google Scholar]
  9. Fikhte B. A., Zaichkin E. I., Ratner E. N. 1973 New Methods for Physical Treatment of Biological Objects for Electron- microscopic Imaging Moscow: Nauka; (in Russian)
    [Google Scholar]
  10. Kimura M., Ohta T. 1972; On the stochastic model for estimation of mutation distance between homologous proteins. J Mol Evol 2:87–90
    [Google Scholar]
  11. Krassilnikov N. A. 1949 Manual for Determination of Bacteria andActinomycetes Moscow/Leningrad: Izdatel’stvo Akademii Nauk SSSR; (in Russian)
    [Google Scholar]
  12. Krassilnikov N. A., Duda V. I., Pivovarov G.E. 1971a; Characteristics of the cell structure of soil anaerobic bacteria producing vesicular caps on their spores. Mikrobiologiya 40:681–685 (in Russian)
    [Google Scholar]
  13. Krassilnikov N. A., Pivovarov G.E., Duda V. I. 1971b; Physiological properties of the soil anaerobic bacteria forming vesicular caps on their spores. Mikrobiologiya 40:896–903 (in Russian)
    [Google Scholar]
  14. Kunstyr I., Schiel R., Kaup F. J., Uhr G., Kirchhoff H. 1988; Giant gram-negative noncultivable endospore-forming bacteria in rodent intestines. Naturwissenschaften 75:525–527
    [Google Scholar]
  15. Marmur J. A. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218
    [Google Scholar]
  16. Pace N. R. 1996; New perspective on the natural microbial word: molecular microbial ecology. ASM News 62:463–470
    [Google Scholar]
  17. Pfennig N. 1965; Anreicherungskulturen fur rote and grime Schwefelbakterien. Zbl Bakt Abt I Orig Suppl. 1:S179–S189
    [Google Scholar]
  18. Pfennig N., Lippert K. D. 1966; Uber das Vitamin B12- Bediirfnis phototropher Schwefelbakterien. Arch Mikrobiol 55:245–256
    [Google Scholar]
  19. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208
    [Google Scholar]
  20. Ryter A., Kellenberger E., Birch-Andersen A., Maaloe Z. 1958; Etude au microscope electronique de plasmas contenant de l’acide desoxyribonuclique. I. Les nucleoides des bacteries au croisance active. Z Naturforsch 136:597–603
    [Google Scholar]
  21. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
    [Google Scholar]
  23. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  24. Vaisman I. S. 1981; Considerations on the value of freeze- etching technique in studying the ultrastructure of some anaerobic bacteria. Acta Histochem Suppl 23:241–247
    [Google Scholar]
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