- International Journal of Systematic and Evolutionary Microbiology
- Volume 49, Issue 3
f Taxonomic characterization of denitrifying bacteria that degrade aromatic compounds and description of Azoarcus toluvorans sp. nov. and Azoarcus toluclasticus sp. nov.
- Authors: Bongkeun Song, Max M. Häggblom, Jizhong Zhou, James M. Tiedje, Norberto J. Palleroni
- Author for correspondence: Norberto J. Palleroni. Tel: +1 732 932 9763 ext. 128. Fax: +1 732 932 8965. e-mail: firstname.lastname@example.org
- Int J Syst Evol Microbiol, July 1999 49: 1129-1140, doi: 10.1099/00207713-49-3-1129
- Subject: New Taxa - Proteobacteria
- Published Online:
A taxonomic characterization of twenty-one strains capable of degrading aromatic compounds under denitrifying conditions, isolated from ten different geographical locations, was performed on the basis of general morphological and physiological characteristics, cellular fatty acids, DNA base composition, small ribosomal (16S) subunit DNA sequences, whole-cell protein patterns and genomic DNA fragmentation analysis, in addition to DNA similarity estimations using hybridization methods. The collection of strains was subdivided into a number of different groups. A first group, consisting of four strains, could be assigned to the previously described species Azoarcus tolulyticus. A second group (five strains) had DNA which reannealed highly to that of strains of the first group, and it is considered to represent a genomova of A. tolulyticus. The third and fourth groups, composed of a total of five strains, represent a new species of Azoarcus, Azoarcus toluclasticus (group 3) and a genomovar of this species (group 4), respectively. Finally, the fifth group, with two strains, corresponds to another new species of the genus Azoarcus, Azoarcus toluvorans. In addition to these five groups, the collection includes five individual strains perhaps representing as many different new species. The above classification is partially consistent with the results of approaches other than DNA-DNA hybridization (electrophoretic patterns of whole-cell proteins and of the fragments obtained after digestion of total DNA with infrequently cutting restriction enzymes). On the other hand, no correlation of these groupings was found in terms of the cellular fatty acid composition. It is also unfortunate that no simple sets of easily determinable phenotypic properties could be defined as being characteristic of each of the groups.
- Keyword(s): aromatic degradation, Azoarcus toluclasticus sp. nov., taxonomy, Azoarcus toluvorans sp. nov.
Copyright © 1999 International Union of Microbiological Societies | Published by the Microbiology Society
Article metrics loading...
Full text loading...
Author and Article Information
/content/journal/ijsem/10.1099/00207713-49-3-1129dcterms_title,dcterms_subject,pub_serialTitlepub_serialIdent:journal/ijsem AND -contentType:BlogPost64
/content/journal/ijsem/10.1099/00207713-49-3-1129dcterms_title,dcterms_subject-pub_serialIdent:journal/ijsem AND -contentType:BlogPost64
Figure data loading....