1887

Abstract

A strictly anaerobic, halotolerant, spindle-shaped rod, designated strain SEBR 4211, was isolated from an African saline oil-producing well. Cells stain Grampositive, which was confirmed by electron microscopy observations. Strain SEBR 4211 was motile by means of one to four peritrichous flagella, had a G+C content of 43 mol% and grew optimally at 37 °C, pH 7.3, with 0 to 3% (w/v) NaCI. It utilized a limited number of carbohydrates (cellobiose, glucose, fructose, mannitol and ribose) and produced acetate, butyrate, CO and H as end products from glucose fermentation. It reduced thiosulfate to sulfide. In the presence of thiosulfate, a decrease in butyrate and an increase in acetate production was observed. Phylogenetically, strain SEBR 4211 was related to members of the low G+C order with as the closest relative (16S rDNA sequence similarity of 90%). On the basis of phenotypic, genotypic and phylogenetic characteristics of the isolate, it is proposed to designate it as a new species of a new genus, gen. nov., as sp. nov. The type strain is SEBR 4211 (= DSM 12116

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1999-07-01
2024-03-28
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References

  1. Andrews K. T., Patel B. K. C. 1996; Fervidobacterium gond- wanense sp. nov., a new thermophilic anaerobic bacterium isolated from nonvolcanically heated geothermal waters of the Great Artesian Basin of Australia. Int J Syst Bacteriol 46:265–269
    [Google Scholar]
  2. Balch W. E., Fox G. E., Magrum J., Woese C. R., Wolfe R. S. 1979; Methanogens: re-evaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  3. Bernard F. P., Connan J., Magot M. 1992; Indigenous microorganisms in connate water of many oil fields: a new tool in exploration and production techniques. In Proceedings of the 67th Annual Technical Conference and Exhibition Society of Petroleum Engineers pp. 1–10 Richardson, TX: Society of Petroleum Engineers;
    [Google Scholar]
  4. Cato E. P., George W. L., Finegold S. M. 1986; Genus Clostridium Prazmowski 1880, 23AL. In Bergefs Manual of Systematic Bacteriology vol. 2 pp. 1141–1200 Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams Wilkins;
    [Google Scholar]
  5. Cayol J.-L., Ollivier B., Lawson Anani Soh A. 7 other authors 1994; Haloincola saccharolytica subsp. senegalensis subsp. nov., isolated from the sediments of a hypersaline lake, and emended description of Haloincola saccharolytica. Int J Syst Bacteriol 44:805–811
    [Google Scholar]
  6. Collins M. D., Lawson P., A, Willems A., Cordoba J. J., Fern- andez-Garayzabal J., Garcia P., Cai J., Hippe H., Farrow J. A. E. 1994; The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826
    [Google Scholar]
  7. Cord-Ruwisch R. 1985; A quick method for the determination of dissolved and precipitated sulfides in cultures of sulfate- reducing bacteria. J Microbiol Methods 4:33–36
    [Google Scholar]
  8. Crolet J.-L., Magot M. 1996; Non SRB sulfidogenic bacteria from oil field production facilities. Mater PerformanceMarch60–64
    [Google Scholar]
  9. Davydova-Charakhch’yan I. A., Mileeva A. N., Mityushina L. L., Belyaev S. S. 1993; Acetogenic bacteria from oil fields of Tartaria and western Siberia. Microbiology (English translation of Mikrobiologiya) 61:306–315
    [Google Scholar]
  10. Fardeau M.-L., Cayol J.-L., Magot M., Ollivier B. 1993; H2 oxidation in the presence of thiosulfate, by a Thermoanaero- bacter strain isolated from an oil-producing well. FEMS Microbiol Lett 113:327–332
    [Google Scholar]
  11. Fardeau M.-L., Faudon C., Cayol J.-L., Magot M., Patel B. K. C., Ollivier B. 1996; Effect of thiosulfate as electron acceptor on glucose and xylose oxidation by Thermoanaerobacter finnii and a Thermoanaerobacter sp. isolated from oil field water. Res Microbiol 147:159–165
    [Google Scholar]
  12. Fardeau M.-L., Ollivier B., Patel B. K. C., Magot M., Thomas P., Rimbault A., Rocchiccioli F., Garcia J.-L. 1997; Thermotoga hypogea sp. nov., a xylanolytic, thermophilic bacterium from an oil-producing well. Int J Syst Bacteriol 47:1013–1019
    [Google Scholar]
  13. Felsenstein J. 1993; phylip (Phylogenetic Inference Package) version 3.51c. Seattle: Department of Genetics, University of Washington;
    [Google Scholar]
  14. Fendrich C., Hippe H., Gottschalk G. 1990; Clostridium halophilium sp. nov. and C. litorale sp. nov., an obligate halophilic and a marine species degrading betaine in the Stickland reaction. Arch Microbiol 154:127–132
    [Google Scholar]
  15. Holloway S. L., Faw G. M., Sizemore R. K. 1980; The bacterial community composition of an active oil field in the Northwestern Gulf of Mexico. Marine Pollut Bull 11:153–156
    [Google Scholar]
  16. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. In Methods in Microbiology pp. 117–132 Vol. 3b Edited by Norris J. R., Ribbons D. W. New York: Academic Press;
    [Google Scholar]
  17. Jeanthon C., Reysenbach A.-L., L’Haridon S., Gambacorta A., Pace N. R., Gibnat P., Prieur D. 1995; Thermotoga subterranea sp. nov., a new thermophilic bacterium isolated from a continental oil reservoir. Arch Microbiol 164:91–97
    [Google Scholar]
  18. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism pp. 211–232 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  19. L’Haridon S., Reysenbach A.-L., Gibnat P., Prieur D., Jeanthon C. 1995; Hot subterranean biosphere in a continental oil reservoir. Nature 377:223–224
    [Google Scholar]
  20. Love C. A., Patel B. K. C., Nichols P. D., Stackebrandt E. 1993; Desulfotomaculum australicum sp. nov., a thermophilic sulfate- reducing bacterium isolated from the Great Artesian Basin of Australia. Syst Appl Microbiol 16:244–251
    [Google Scholar]
  21. Magot M., Carreau L., Cayol J.-L., Ollivier B., Crolet J.-L. 1994; Sulphide-producing, not sulphate-reducing anaerobic bacteria presumptively involved in bacterial corrosion. In Proceedings of the 3rd European Federation of Corrosion Workshop on Microbiol Corrosion. Edited by Sequeira C. A. C. London: The Institute of Materials;
    [Google Scholar]
  22. Magot M., Fardeau M.-L., Arnauld O., Lanau C., Ollivier B., Thomas P., Patel B. K. C. 1997a; Spirochaeta smaragdinae sp. nov., a new mesophilic strictly anaerobic spirochete from an oil field. FEMS Microbiol Lett 155:185–191
    [Google Scholar]
  23. Magot M., Ravot G., Campaignolle X., Ollivier B., Patel B. K. C., Fardeau M.-L., Thomas P., Crolet J.-L., Garcia J.-L. 1997b; Dethiosulfovibrio peptidovorans gen. nov., sp. nov., a new anaerobic, slightly halophilic, thiosulfate-reducing bacterium from corroding offshore oil wells. Int J Syst Bacteriol 47:818–824
    [Google Scholar]
  24. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1996; The ribosomal database project (RDP). Nucleic Acids Res 24:82–85
    [Google Scholar]
  25. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
    [Google Scholar]
  26. Ravot G., Magot M., Fardeau M.-L., Patel B. K. C., Prensier G., Egan A., Garcia J.-L., Ollivier B. 1995a; Thermotoga elfii sp. nov., a novel thermophilic bacterium from an African oil- producing well. Int J Syst Bacteriol 45:308–314
    [Google Scholar]
  27. Ravot G., Ollivier B., Magot M., Patel B. K. C., Crolet J.-L., Fardeau M.-L., Garcia J.-L. 1995b; Thiosulfate reduction, an important physiological feature shared by members of the order Thermotogales. Appl Environ Microbiol 61:2053–2055
    [Google Scholar]
  28. Ravot G., Magot M., Ollivier B., Patel B. K. C., Ageron E., Grimont P. A. D., Thomas P., Garcia J.-L. 1997; Haloanaero- bium congolense sp. nov., an anaerobic, moderately halophilic, thiosulfate- and sulfur-reducing bacterium from an African oil field. FEMS Microbiol Lett 147:81–88
    [Google Scholar]
  29. Redburn A. C., Patel B. K. C. 1993; Phylogenetic analysis of Desulfotomaculum thermobenzoicum using polymerase chain reaction-amplified 16S rRNA-specific DNA. FEMS Microbiol Lett 113:81–86
    [Google Scholar]
  30. Stetter K. O., Huber R., Blochl E., Kurr M., Eden R., D„ Fielder M., Cash H., Vance I. 1993; Hyperthermophilic Archaea are thriving in deep North Sea and Alaskan oil reservoirs. Nature 365:743–745
    [Google Scholar]
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