1887

Abstract

Two novel hyperthermophilic, rod-shaped crenarchaeotes were isolated from an acidic hot spring in the Philippines. Cells were mostly straight or slightly curved rods 0.4-0.7 μm in width. Bent cells, branched cells, and cells bearing globular bodies were commonly observed. The isolates were heterotrophs and grew anaerobically and microaerobically. The addition of archaeal cell extract or a vitamin mixture to the medium significantly stimulated growth. The isolates grew over a temperature range of 60-92 °C, and optimally around 85 °C and grew over a pH range of 2.3-6.4, and optimally at pH 3.7-4.2. The isolates utilized glycogen, gelatin, beef extract, peptone, tryptone and yeast extract as carbon sources. They required sulfur, thiosulfate or sulfate as electron acceptors. The lipids mainly consisted of various cyclized glycerolbisdiphytanyl-glycerol tetraethers. The G+C content of the genomic DNAs was 43 mol%. The 16S rDNA contained two small introns. The comparison of the 16S rDNA exon sequences revealed that they represented an independent lineage in the family . The two strains were included in a single species because of high levels of DNA-DNA relatedness. From these results, gen. nov., sp. nov. is proposed in the family to accommodate these isolates. The type strain of is strain IC-167 (= JCM 10307 =MCC-UPLB 1200 = ANMR 0178).

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1999-07-01
2024-03-28
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References

  1. Balch W. E., Fox G. E., Magrum L. J., Woese C. R., Wolfe R. S. 1979; Methanogens: re-evaluation of a unique biological group. Microbiol Rev 43:260–296
    [Google Scholar]
  2. Bonch-Osmolovskaya E. A., Miroshnichenko M., L, Kostrikina N. A., Chernych N. A., Zavarzin G. A. 1990; Thermoproteus uzoniensis sp. nov., a new extremely thermophilic archae- bacterium from Kamchatka continental hot springs. Arch Microbiol 154:556–559
    [Google Scholar]
  3. Brock T. D., Brock K. M., Belly R. T., Weiss R. L. 1972; Sulfolobus: a new genus of sulfur-oxidizing bacteria living at low pH and high temperature. Arch Microbiol 84:54–68
    [Google Scholar]
  4. Burggraf S., Larsen N., Woese C. R., Stetter K. O. 1993; An intron within the 16S ribosomal RNA gene of the archaeon Pyrobaculum aerophilum. Proc Natl Acad Sci USA 90:2547–2550
    [Google Scholar]
  5. Burggraf S., Huber H., Stetter K. O. 1997; Reclassification of the crenarchaeal orders and families in accordance with 16S rRNA sequence data. Int J Syst Bacteriol 47:657–660
    [Google Scholar]
  6. Dalgaard J. Z., Garrett R. A. 1992; Protein-coding introns from the 23 S rRNA-encoding gene form stable circles in the hyperthermophilic archaeon Pyrobaculum organotrophum. Gene 121:103–110
    [Google Scholar]
  7. Huber R., Kristjansson J. K., Stetter K. O. 1987; Pyrobaculum gen. nov., a new genus of neutrophilic, rod-shaped archae- bacteria from continental solfataras growing optimally at 100 °C. Arch Microbiol 149:95–101
    [Google Scholar]
  8. Itoh T., Suzuki K., Nakase T. 1998a; Thermocladium modestius gen. nov., sp. nov., a new genus of rod-shaped, extremely thermophilic crenarchaeote. Int J Syst Bacteriol 48:879–887
    [Google Scholar]
  9. Itoh T., Suzuki K., Nakase T. 1998b; Occurrence of introns in the 16S rRNA genes of members of the genus Thermoproteus. Arch Microbiol 170:155–161
    [Google Scholar]
  10. Kjems J., Garrett R. A. 1985; An intron in the 23S ribosomal RNA gene of the archaebacterium Desulfurococcus mobilis. Nature 318:675–677
    [Google Scholar]
  11. Kjems J., Garrett R. A. 1988; Novel splicing mechanism for the ribosomal RNA intron in the archaebacterium Desulfurococcus mobilis. Cell 54:693–703
    [Google Scholar]
  12. Kjems J., Garrett R. A. 1991; Ribosomal RNA introns in archaea and evidence for RNA conformational changes associated with splicing. Proc Natl Acad Sci USA 88:439–443
    [Google Scholar]
  13. Kjems J., Larsen N., Dalgaard J. Z., Garrett R. A., Stetter K. O. 1992; Phylogenetic relationships amongst the hyper- thermophilic archaea determined from partial 23 S rRNA gene sequences. Syst Appl Microbiol 15:203–208
    [Google Scholar]
  14. Lykke-Andersen J., Garrett R. A. 1994; Structural characteristics of the stable RNA introns of archaeal hyper- thermophiles and their splicing junctions. J Mol Biol 243:846–855
    [Google Scholar]
  15. Nomura N., Sako Y., Uchida A. 1998; Molecular characterization and postsplicing fate of three introns within the single rRNA operon of the hyperthermophilic archaeon Aeropyrum pernix Kl. J Bacteriol 180:3635–3643
    [Google Scholar]
  16. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  17. Stetter K. O. 1986; Diversity of extremely thermophilic archae- bacteria. In Thermophiles: General Molecular, and Applied Microbiology pp. 39–74 Edited by Brock T. D. New York: Wiley;
    [Google Scholar]
  18. Stetter K. O. 1996; Hyperthermophilic procaryotes. FEMS Microbiol Rev 18:149–158
    [Google Scholar]
  19. Vdlkl P., Huber R., Drobner E., Rachel R., Burggraf S., Trincone A., Stetter K. O. 1993; Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum. Appl Environ Microbiol 59:2918–2926
    [Google Scholar]
  20. Woese C. R. 1993; The archaea: their history and significance. In The Biochemistry of Archaea (Archaebacteria) pp. vii–xxix Edited by Kate M., Kushner D. J., Matheson A. T. Amsterdam: Elsevier;
    [Google Scholar]
  21. Woese C. R., Kandler O., Wheelis M. L. 1990; Towards a natural system of organisms: proposal for the domains Archaea, Bacteria, and Eucarya. Proc Natl Acad Sci USA 87:4576–4579
    [Google Scholar]
  22. Zillig W. 1989; Genus I. Thermoproteus Zillig and Stetter 1982, 267vp. In Bergey’s Manual of Systematic Bacteriology vol. 3 p. 2241 Edited by Staley J. T., Bryant M. P., Pfennig N., Holt J. G. Baltimore: Williams Wilkins;
    [Google Scholar]
  23. Zillig W., Stetter K. O., Schafer W., Janekovic D., Wunderl S., Holz I., Palm P. 1981; Thermoproteales: a novel type of extremely thermoacidophilic anaerobic archaebacteria isolated from Icelandic solfataras. Zentbl Bakteriol Mikrobiol Hygl Abt Orig C 2:205–227
    [Google Scholar]
  24. Zillig W., Gierl A., Schreiber G., Wunderl S., Janekovic D., Stetter K. O., Klenk H. P. 1983; The archaebacterium Thermofilum pendens represents a novel genus of the thermophilic, anaerobic sulfur respiring Thermoproteales. Syst Appl Microbiol 4:79–87
    [Google Scholar]
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