1887

Abstract

The phylogenetic relationships among 36 validly described species or subspecies within the genus were investigated by cloning an sequencing their 60 kDa heat-shock protein (HSP60) genes using a set of universal degenerate HSP60 PCR primers. The cloned partial HSP60 DNA sequences from nine strains were highly conserved (97-100% DNA sequence similarity; mean 98%), indicating that the HSP60 gene of multiple isolates within the same species have little microheterogeneity. At the subspecies level, DNA sequence similarity among members of and ranged from 91 to 98%. At the interspecies level, sequence similarity among 23 distinct species of staphylococci ranged from 7 to 93% (mean 82%). By comparison, the highest sequence similarity of and with members within the genus was only 70 and 59%, respectively. Importantly, phylogenetic analysis based on the neighbour-joining distance method revealed remarkable concordance between the tree derived from partial HSP60 gene sequences and that based on genomic DNA-DNA hybridization, while 16S rRNA gene sequences correlated less well. The results demonstrate that DNA sequences from the highly conserved and ubiquitous HSP60 gene offer a convenient and accure tool for species-specific identification and phylogenetic analysis of staphylococci.

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1999-07-01
2024-03-28
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References

  1. Altschul S. F., Madden T., L, Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
    [Google Scholar]
  2. Birnbaum D., Kelly M., Chow A. W. 1991; Epidemiologic typing systems for coagulase-negative staphylococci. Infect Control Hosp Epidemiol 12:319–326
    [Google Scholar]
  3. Birnbaum D., Herwaldt L., Low D. E., Noble M., Pfaller M., Sherertz R., Chow A. W. 1994; Efficacy of microbial identification system for epidemiologic typing of coagulase- negative staphylococci. J Clin Microbiol 32:2113–2119
    [Google Scholar]
  4. Endl J., Seidl H. P., Fiedler F., Schleifer K. H. 1983; Chemical composition and structure of cell wall teichoic acids of staphylococci. Arch Microbiol 135:215–223
    [Google Scholar]
  5. Euzeby J. P. 1997; List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592
    [Google Scholar]
  6. Felsenstein J. 1995; phylip - Phylogeny Inference Package (version 3.57c). Seattle, WA: University of Washington;
    [Google Scholar]
  7. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specified tee topology. Syst Zool 20:406–416
    [Google Scholar]
  8. Fox G. E., Wisotzkey J. D., Jurtshuk P. Jr 1992; How close is close: 16S rRNA sequence identity may not be sufficient to guarantee soecies identity. Int J Syst Bacteriol 42:166–170
    [Google Scholar]
  9. Goh S. H., Potter S., Wood J. O., Hemmingsen S. M., Reynolds R. P., Chow A. W. 1996; HSP60 gene sequences as universal targets for microbial species identification: studies with coagulase-negative staphylococci. J Clin Microbiol 34:818–823
    [Google Scholar]
  10. Goh S. H., Santucci Z., Kloos W. E., Faltyn M., George C. G., Driedger D., Hemmingsen S. M. 1997; Identification of Staphylococcus species and subspecies by the chaperonin 60 gene identification method and reverse checkerboard hybridization. J Clin Microbiol 35:3116–3121
    [Google Scholar]
  11. Gribaldo S., Cookson B., Saunders N., Marples R., Stanley J. 1997; Rapid identification by specific PCR of coagulase- negative staphylococcal species important in hospital infection. J Med Microbiol 46:45–53
    [Google Scholar]
  12. Gupta R. S. 1995; Evolution of the chaperonin families (Hsp60, HsplO and Tcp-1) of proteins and the origin of eukaryotic cells. Mol Microbiol 15:1–11
    [Google Scholar]
  13. Ieven M., Verhoeven J., Pattyn S. R., Goosens H. 1995; Rapid and economical method for species identification of clinically significant coagulase-negative staphylococci. J Clin Microbiol 33:1060–1063
    [Google Scholar]
  14. Jukes T. H., Cantor C. R. 1969; Evolution of protein molecules. In Mammalian Protein Metabolism vol. 3 pp. 21–132 Edited by Munro H. N. New York: Academic Press;
    [Google Scholar]
  15. Kloos W. E. 1997; Taxonomy and systematics of staphylococci indigenous to humans. In The Staphylococci in Human Disease pp. 113–137 Edited by Crossley K. B., Archer G. L. New York: Churchill Livingstone;
    [Google Scholar]
  16. Kloos W. E., Bannerman T. L. 1995; Staphylococcus and Micrococcus. Manual of Clinical Microbiology, 6th. edn, pp. 282–298 Edited by Murray P. R., Baron E. J., Pfaller M. A., Tenover F. C., Yolken R. H. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Kloos W. E., Ballard D. N., George C. G., Webster J. A., Hubner R. J., Ludwig W., Schleifer K.-H., Fiedler F., Schubert K. 1998; Delimiting the genus Staphylococcus through description of Macrococcus caseolyticus gen. nov., comb. nov. and Macrococcus equipercicus sp. nov., Macrococcus bovicus sp. nov. and Macrococcus carouselicus sp. nov. Int J Syst Bacteriol 48:859–877
    [Google Scholar]
  18. Martin de Nicolas M. M., Vindel A., Saez-Nieto J. A. 1995; Epidemiological typing of clinically significant strains of coagulase-negative staphylococci. J Hosp Infect 29:35–43
    [Google Scholar]
  19. O’Donnell A. G., Nahaie M. R., Goodfellow M., Minnikin D. E., Hajek V. 1985; Numerical analysis of fatty acid profiles in the identification of staphylococci. J Gen Microbiol 131:2023–2033
    [Google Scholar]
  20. Olsen G. J., Woese C. R., Overbreek R. 1994; The winds of (evolutionary) change: breathing new life into microbiology. J Bacteriol 176:1–6
    [Google Scholar]
  21. Pai S., Esen N., Pan X., Musser J. M. 1997; Routine rapid mycobacterium species assignment based on species-specific allelic variation in the 65-kilodalton heat shock protein gene (hsp65). Arch Pathol Lab Med 121:859–864
    [Google Scholar]
  22. Pillet J., Orta B. 1981; Species and serotypes in coagulase-negative Staphylococci. In. Staphylococci and Staphylocoocal Infections. pp. 147–152 Zentralblatt fur Bakteriologie, Mikro- biologie und Hygiene. I. Abteilung Supplemente vol. 10 Edited by Jeljaszewicz J., Hryniewicz W., Switalski L. M., Szmigielski S. Stuttgart New York: Gustav Fischer Verlag;
    [Google Scholar]
  23. Rosypal S., Rosypalova A., Horejs J. 1966; The classification of micrococci and staphylococci based on their DNA base composition and Adansonian analysis. J Gen Microbiol 44:281–292
    [Google Scholar]
  24. Schumacher-Perdreau F., Rotering H., Pulverer G. 1983; Cell wall analysis and taxonomy of staphylococci. Zentbl Bakteriol Mikrobiol Hyg 1 Abt Orig A 256:25–36
    [Google Scholar]
  25. Smith S. 1997 Genetic Data Environment. Harvard Genome Laboratory Chicago: University of Illinois;
    [Google Scholar]
  26. Stackebrandt E., Goebel B. M. 1994; A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
  27. Stackebrandt E., Liesack W., Witt D. 1992; Ribosomal RNA and rDNA sequence analysis. Gene 115:255–260
    [Google Scholar]
  28. Steingrube V. A., Gibson J. L., Brown B. A., Zhang Y., Wilson R. W., Rajagopalan M., Wallace R. J. Jr 1995; PCR amplification and restriction endonuclease analysis of a 65-kilodalton heat shock protein gene sequence for taxonomic separation of rapidly growing mycobacteria. J Clin Microbiol 33:149–153
    [Google Scholar]
  29. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 224673–4680
    [Google Scholar]
  30. Viale A. M., Arakaki A. K., Soncini F. C., Ferreyra R. G. 1994; Evolutionary relationships among eubacterial groups as inferred from GroEL (chaperonin) sequence comparisons. Int J Syst Bacteriol 44:527–533
    [Google Scholar]
  31. Wayne L. G., Brenner D. J., Colwell R. R. 9 other authors 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464
    [Google Scholar]
  32. Yamamoto S., Harayama S. 1995; PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Appl Environ Microbiol 61:1104–1109
    [Google Scholar]
  33. Zakrzewska-Czerwinska J., Gaszewska-Mastalarz A., Lis B., Gamian A., Mordarski M. 1995; Staphylococcus pulvereri sp. nov., isolated from human and animal specimens. Int J Syst Bacteriol 45:169–172
    [Google Scholar]
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