1887

Abstract

Two strictly aerobic, heterotrophic and mesophilic new organisms, strains Kitami A1 and Kitami C2, were isolated from the wastewater of a sugar-beet factory in Kitami City, Hokkaido, Japan. In batch cultures, these organisms produced both insoluble and soluble exopolysaccharides (EPSs) utilizing sucrose as the sole carbon source. The G+C contents of the strains Kitami C2 and Kitami A1 were 69·2 mol%. Both strains had anteiso-C15:0 acid, anteiso-C17:0 acid and iso-C16:0 as major components. The major isoprenoid quinones from these strains included menaquinone-11 and menaquinone-12. Physiological and biochemical characterization, phylogenetic analysis and DNA-DNA relatedness indicated that these two organisms are new species of the genus , for which the name is proposed. The type strain of is strain Kitami C2 (= JCM 10270).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-4-1353
1999-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/4/ijs-49-4-1353.html?itemId=/content/journal/ijsem/10.1099/00207713-49-4-1353&mimeType=html&fmt=ahah

References

  1. Albersheim P., Nevins D. J., English P. D., Karr A. 1967; A method for the analysis of sugars in plant cell-wall polysaccharides by gas-chromatography. Carbohydr Res 5:340–345
    [Google Scholar]
  2. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Calorimetric method for determination of sugars and related substances. Anal Chern 28:350–356
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229
    [Google Scholar]
  4. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16:111–120
    [Google Scholar]
  5. Komagata K. 1985 Bacteria (1): the aerobic bacteria. Classification and Identification of Microorganisms 299–161 Edited by Hasegawa T. Tokyo: Gakkai Schuppan; in Japanese
    [Google Scholar]
  6. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J Biol Chern 193:265–275
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218
    [Google Scholar]
  9. Patel G. B. 1984; Characterization and nutritional properties of Methanothrix concilii sp. nov., a mesophilic acetoclastic methanogen. Can J Microbiol 30:1383–1396
    [Google Scholar]
  10. Raguenes G., Pignet P., Gauthier G., Peres A., Christen R., Rougeaux H., Barbier G., Guezennec J. 1996; Description of a new polymer-secreting bacterium from a deep-sea hydrothermal vent, Altermonas macleodii subsp. fijiensis, and preliminary characterization of the polymer. Appl Environ Microbiol 62:67–73
    [Google Scholar]
  11. Raguenes G., Christen R., Guezennec J., Pignet P., Barbier G. 1997a; Vibrio diabolicus sp. nov., a new polysaccharide secreting organism isolated from a deep-sea hydrothermal vent polychaete annelid, Alvinellapompejana. Int J Syst Bacteriol 47:989–995
    [Google Scholar]
  12. Raguenes G. H. C., Peres A., Ruimy R., Pignet P., Christen R., Loaec M., Rougeaux H., Barbier G., Guezennec J. G. 1997b; Alteromonas infernus sp. nov., a new polysaccharide-producing bacterium isolated from a deep-sea hydrothermal vent. J Appl Microbiol 82:422–430
    [Google Scholar]
  13. Saito N., Nei M. 1987; A neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425
    [Google Scholar]
  14. Schleifer K. H., Kandler O. 1972; Peptidoglycan type of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477
    [Google Scholar]
  15. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K. 1997; Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47:289–298
    [Google Scholar]
  16. Suzuki K., Komagata K. 1983; Taxonomic significance of cellular fatty acid composition in some coryneform bacteria. Int J Syst Bacteriol 33:188–200
    [Google Scholar]
  17. Takeuchi M., Hatano K. 1998a; Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium. Int J Syst Bacteriol 48:739–747
    [Google Scholar]
  18. Takeuchi M., Hatano K. 1998b; Proposal of six new species in the genus Microbacterium and transfer of Flavobacterium marinotypicum ZoBell and Upham to the genus Microbacterium as Microbacterium maritypicum comb. nov. Int J Syst Bacteriol 48:973–982
    [Google Scholar]
  19. Tamaoka J., Komagata K. 1984; Determination of base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128
    [Google Scholar]
  20. Thomas M. J., Albersheim P. 1972; A gas chromatographic method for the determination of aldose and uronic acid constituents of plant cell wall polysaccharides. Plant Physiol 49:926–936
    [Google Scholar]
  21. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighing, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
    [Google Scholar]
  22. Uchida K., Aida K. 1977; Acyl type of bacterial cell wall: its simple identification by a colorimetric method. J Gen Appl Microbiol 23:249–260
    [Google Scholar]
  23. Yokota A., Takeuchi M., Weiss N. 1993a; Proposal of two new species in the genus Microbacterium: Microbacterium dextranolyticum sp. nov. and Microbacterium aurum sp. nov. Int J Syst Bacteriol 43:549–554
    [Google Scholar]
  24. Yokota A., Takeuchi M., Sakane T., Weiss N. 1993b; Proposal of six new species in the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov. Int J Syst Bacteriol 43:555–564
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-4-1353
Loading
/content/journal/ijsem/10.1099/00207713-49-4-1353
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error