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Volume 49, Issue 4

sp. nov., a soft rot pathogen of Free

Abstract

A novel bacterium has been found that causes a soft rot disease of , the cultivated mushroom. It has been characterized using nutritional, physiological, chemical and molecular techniques. Based on these data, it was shown to have many characteristics in common with members of the genus . Despite similarities to the only described species within this genus, , there were a number of differences between the mushroom pathogen isolated and this species. Despite the high degree of genotypic similarity between members of the genus and , as evidenced by DNA-RNA hybridization, and the high degree of 16S rDNA sequence similarity between members of the genera and , as well as the generically misnamed , it was possible to show that members of the genus could be easily distinguished from these taxa. The data also indicated that the mushroom pathogenic strains represent a novel species within the genus for which the name sp. nov. is proposed. The type strain of this species has been deposited in the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany, as DSM 9628 and at the National Collection of Plantpathogenic bacteria, UK, as NCPPB 3945. To aid practical control of the disease, the effect of the relative humidity on symptom expression on was determined.

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Keyword(s): 16S rDNA sequence , Agaricus bisporus pathogen , chemotaxonomy , Janthinobacterium agaricidamnosum and taxonomy
© 1999 International Union of of Microbiological Societies
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1999-10-01
2024-04-26
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References

  1. Allison M. J., Dawson K. A., Mayberry W. R., Foss J. G. 1985; Oxalobacter formigenes gen. nov., sp. nov.: oxalate degrading anaerobes that inhabit the gastrointestinal tract. Arch Microbiol 141:1–7
     [Google Scholar]
  2. ASTM 1963; Recommended practice for maintaining constant relative humidity by means of aqueous solutions. ASTM (Am Soc Test Mater)E104–E151
     [Google Scholar]
  3. Baldani J. I., Pot B., Kirchhof G.8 other authors 1996; Emended description of Herbaspirillum·. inclusion of (Pseudomonas] rubrisubalbicans, a mild plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF Group 1) as Herbaspirillum species 3. Int J Syst Bacteriol 46:802–810
     [Google Scholar]
  4. Bowman J. P., Sly L. I., Hayward A. G, Spiegel Y., Stackebrandt E. 1993; Telluria mixta (Pseudomonas mixta Bowman, Sly & Hayward 1988) gen. nov., comb, nov., and Telluria chitinolytica sp. nov., soil-dwelling organisms which actively degrade polysaccharides. Int J Syst Bacteriol 43:120–124
     [Google Scholar]
  5. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466
     [Google Scholar]
  6. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implications. Microbiol Rev 45:316–354
     [Google Scholar]
  7. Cox A. D., Wilkinson S. G. 1989; Polar lipids and fatty acids of Pseudomonas cepacia. Biochim Biophys Acta 1001:60–67
     [Google Scholar]
  8. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142
     [Google Scholar]
  9. De Ley J., Segers P., Gillis M. 1978; Intra and intergeneric similarities of Chromobacterium and Janthinobacterium ribosomal ribonucleic acid cistrons. Int J Svst Bacteriol 28:154–168
     [Google Scholar]
  10. Devereux R., He S.-H., Doyle C. L., Orkland S., Stahl D. A., LeGall J., Whitman W. B. 1990; Diversity and origin of Desulfovibrio species: phylogenetic definition of a family. J Bacteriol 172:3609–3619
     [Google Scholar]
  11. Escara J. F., Hutton J. R. 1980; Thermal stability and renaturation of DNA in dimcthylsulphoxide solutions: acceleration of renaturation rate. Biopolymers 19:1315–1327
     [Google Scholar]
  12. Fletcher J. T., White P. F., Gaze R. H. 1989 Mushrooms: Pests and Disease Control, 2nd.174 Andover: Intercept;
     [Google Scholar]
  13. Fry N. K., Warwick S., Saunders N. A., Embley T. M. 1991; The use of 16S rRNA analyses to investigate the phylogeny of the family Legionellaceae. J Gen Microbiol 137:1215–1222
     [Google Scholar]
  14. Galbraith L., Wilkinson S. G. 1991; Polar lipids and fatty acids of Pseudomonas caryophylli, Pseudomonas gladioli and Pseudomonas pickettii. J Gen Microbiol 137:197–202
     [Google Scholar]
  15. Gandy D. E. 1968; A technique for screening bacteria causing Brown blotch of cultivated mushrooms. Rep Glasshouse Crops Res Inst1967150–154
     [Google Scholar]
  16. Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. 1981 Manual of Methods for General Microbiology Washington, DC: American Society for Microbiology;
     [Google Scholar]
  17. Hermans C. 1988 Climate and cultivation techniques. The Cultivation of Mushrooms213–248 Edited by van Griensven L. J. L. D. Sussex, UK: Darlington Mushroom Laboratories;
     [Google Scholar]
  18. Hiraishi A., Shin Y. K., Sugiyama J., Komagata K. 1992; Isoprenoid quinones and fatty acids of Zoogloea. Antonie Leeuwenhoek 61:231–236
     [Google Scholar]
  19. Hiraishi A., Shin Y. K., Sugiyama J. 1997; Proposal to reclassify Zoogloea ramigera IAM 12670 (P. R. Dugan 115) as Duganella zoogloeoides gen. nov., sp. nov. Int J Syst Bacteriol 47:1249–1252
     [Google Scholar]
  20. Inglis P. W., Burden J. L., Peberdy J. F. 1996; Evidence for the association of the enteric bacterium Ewingella americana with internal stipe necrosis of Agaricus bisporus. Microbiology 142:3253–3260
     [Google Scholar]
  21. Jahnke K. D. 1992; basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford system 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15:61–73
     [Google Scholar]
  22. Johnson J. L. 1984 Nucleic acids in bacterial classification. Bergey’s Manual of Systematic Bacteriology 18–11 Edited by Kreig N. R., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  23. King E. O., Ward M. K., Raney D. E. 1954; Two simple media for the demonstration of pyocyanin and fluorescein. J Lab Clin Med 44:301–307
     [Google Scholar]
  24. Lelliott R. A., Stead D. E. 1987 Methods for the Diagnosis of Bacterial Diseases of Plants Oxford: Blackwell Scientific Publications;
     [Google Scholar]
  25. Lincoln S. P., Fermor T. R., Stead D. E., Sellwood J. E. 1991; Bacterial soft rot of Agaricus bitorquis. Plant Pathol 40:136–144
     [Google Scholar]
  26. Logan N. A., Moss M. O. 1992 Identification of Chromobacterium, Janthinobacterium, and lodobacter species. Identification Methods in Applied and Environmental Microbiology, Technical Series vol. 29183–192 Edited by Board R. G., Jones D., Skinner F. A. Bedford, UK: Society for Applied Bacteriology;
     [Google Scholar]
  27. Ludwig W., Strunk O. 1996; arb: a software environment for sequence data. http://www.mikro.biologie.tu-muenchen.de/pub/ARB/documentation/arb.ps Technische Universität München; Munich, Germany:
  28. Maidak B. L., Olsen G. J., Larsen N., Overbeek R., McCaughey M. J., Woese C. R. 1997; The RDP (Ribosomal Database Project). Nucleic Acids Res 25:109–111
     [Google Scholar]
  29. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 4:109–118
     [Google Scholar]
  30. Mergaert J., Schirmer A., Hauben L., Mau M., Hoste B., Kersters K., Jendrossek D., Swings J. 1996; Isolation and identification of poly(3-hydroxyvalerate)-degrading strains of Pseudomonas lemoignei. Int J Syst Bacteriol 46:769–773
     [Google Scholar]
  31. Mesbah M., Premachandran U., Whitman W. 1989; Precise measurement of the G + C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39:159–167
     [Google Scholar]
  32. Moss M. O., Ryall C. 1981 The genus Chromobacterium. The Prokaryotes1355–1364 Edited by Starr M. P., Stolp H., Triiper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  33. Moss M. O., Ryall C., Logan N. A. 1978; The classification and characterization of chromobacteria from a lowland river. J Gen Microbiol 105:11–21
     [Google Scholar]
  34. Moss C. W., Dees S. B., Guerrans G. O. 1980; Gas chromatography of bacterial fatty acids with a fused silica capillary column. J Clin Microbiol 12:127–130
     [Google Scholar]
  35. Neumann B., Pospiech A., Schairer H. U. 1992; Rapid isolation of genomic DNA from Gram-negative bacteria. Trends Genet 8:332–333
     [Google Scholar]
  36. Oyaizu H., Komagata K. 1983; Grouping of Pseudomonas species on the basis of cellular fatty acid composition and the quinone system with special reference to the existence of 3- hydroxy fatty acids. J Gen Appl Microbiol 29:17–40
     [Google Scholar]
  37. Paine S. G. 1919; Studies in bacteriosis. II. A brown blotch disease of cultivated mushrooms. Ann Appl Biol 5:206–219
     [Google Scholar]
  38. Rainey F. A., Stackebrandt E. 1993; 16S rDNA analysis reveals phylogenetic diversity among the polysaccharolytic Clostridia. FEMS Microbiol Lett 113:125–128
     [Google Scholar]
  39. Rainey F. A., Dorsch M., Morgan H. W., Stackebrandt E. 1992a; 16S rDNA analysis of Spirochaeta thermophila’, position and implications for the systematics of the order Spirochaetales. Syst Appl Microbiol 16:224–226
     [Google Scholar]
  40. Rainey P. B., Brodey C. L., Johnstone K. 1992b; Biology of Pseudomonas tolaasii, cause of Brown Blotch disease of the cultivated mushroom. Adv Plant Pathol 8:95–117
     [Google Scholar]
  41. Rainey P. B., Moxon E. R., Thompson I. P. 1993; Intraclonal polymorphism in bacteria. Adv Microb Ecol 13:263–300
     [Google Scholar]
  42. Shivaji S., Ray M. K., Seshu Ku marg G., Reddy G. S. N., Saisree L., Wynn-Williams D. D. 1991; Identification of Janthinobacterium lividum from the soils of the island of Scotia Ridge and from Antarctic peninsula. Polar Biol 11:267–271
     [Google Scholar]
  43. Sivendra R., Lo H. S., Lim K. T. 1975; Identification of Chromobacterium violaceum·. pigmented and non-pigmented strains. J Gen Microbiol 90:21–31
     [Google Scholar]
  44. Sneath P. H. A. 1984 Genus Janthinobacterium De Ley, Segers & Gillis 1978, 164AL. Bergey’s Manual of Systematic Bacteriology 1376–377 Edited by Kreig N. R., Holt J. G. Baltimore: Williams & Wilkins;
     [Google Scholar]
  45. Stead D. E. 1992; Grouping of plant-pathogenic and some other Pseudomonas spp. by using cellular fatty acid profiles. Int J Syst Bacteriol 42:281–295
     [Google Scholar]
  46. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128
     [Google Scholar]
  47. Tindall B. J. 1990a; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130
     [Google Scholar]
  48. Tindall B. J. 1990b; Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66:199–202
     [Google Scholar]
  49. Tindall B. J. 1994 Chemical analysis of Archaea and Bacteria ‘. a critical evaluation of its use in taxonomy and identification. Bacterial Diversity and Systematics, FEMS Symposium No. 75 Edited by Priest F. G., Ramos-Cormenzana A., Tindall B. J. New York: Plenum;
     [Google Scholar]
  50. Woese C. R. 1987; Bacterial evolution. Microbiol Rev 51:221–227
     [Google Scholar]
  51. Wong W. C., Preece T. F. 1979; Identification of Pseudomonas tolaasii: the white line in agar and mushroom tissue block rapid pitting test. J Appl Bacteriol 47:401–407
     [Google Scholar]
  52. Wong W. C., Fletcher J. T., Unsworth B. A., Preece T. F. 1982; A note on ginger blotch, a new disease of the cultivated mushroom Agaricus bisporus. J Appl Bacteriol 52:43–48
     [Google Scholar]
  53. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T., Arakawa M. 1992; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36:1251–1275
     [Google Scholar]
  54. Yabuuchi E., Kosako Y., Yano I., Hotta H., Nishiuchi Y. 1995; Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. nov.: proposal of Ralstonia pickettii (Ralston, Palleroni and Doudoroff 1973) comb, nov., Ralstonia solanacearum (Smith 1896) comb. nov. and Ralstonia eutropha (Davis 1969) comb. nov. Microbiol Immunol 39:897–904
     [Google Scholar]
  55. Yokota A., Akagawa-Matsushita M., Hiraishi A., Katayama Y., Urakami T., Yamasato K. 1992; Distribution of quinone systems in micro-organisms: Gram-negative eubacteria. Bull Jpn Fed Cult Coll 8:136–171
     [Google Scholar]
  56. Young J. F. 1967; Humidity control in the laboratory using salt solutions - a review. J Appl Chern 17:241–245
     [Google Scholar]
  57. Young J. M. 1970; Drippy gill: a bacterial disease of mushrooms caused by Pseudomonas agarici n. sp. NZ J Agric Res 13:977–990
     [Google Scholar]
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Janthinobacterium agaricidamnosum sp. nov., a soft rot pathogen of Agaricus bisporus
Int J Syst Evol Microbiol 49, 1577 (1999); https://doi.org/10.1099/00207713-49-4-1577
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