1887

Abstract

’ is an uncultured, tightly spiral micro-organism that has been associated with ulcer disease in the stomachs of pigs. It was the purpose of this study to determine the phylogenetic position of ‘’. Stomachs of five slaughterhouse pigs, originating from different Belgian and Dutch farms, were selected on the basis of the presence of ‘’-like bacteria, as demonstrated by biochemical, immunohistochemical and electron microscopical data. Bacterial 16S rDNA was amplified by PCR using broadrange primers and five helicobacter-like sequences were determined either by direct or indirect sequence analysis. An inter-sequence homology of 99·7% was observed, suggesting that the sequences originated from strains belonging to a single species. Phylogenetic analysis of the consensus sequence placed the organism within the genus , where it formed a distinct sub-group together with other gastrospirillum-like bacteria ( and ‘’ types 1 and 2). Diagnostic PCR primers and a probe were developed that differentiated the porcine sequences from all known helicobacters. These results indicate that the porcine sequences represent a single taxon within the genus . The low similarity level towards (96·6%), its closest validly named neighbour, strongly suggests that this taxon is a novel hybridization experiments linked the reference sequence to the ‘’-like bacteria. On the basis of these results, we propose the name ‘ Helicobacter suis’ for this gastric helicobacter from pigs.

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1999-10-01
2024-03-28
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References

  1. Altschul S. F., Madden T. L, Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
    [Google Scholar]
  2. Barbosa A. J., Silva J. G, Nogueira A. M., Paulino Junior E., Miranda C. R. 1995; Higher incidence of Gastrospirillum sp. in swine with gastric ulcer of the pars oesophagea. Vet Pathol 32:134–139
    [Google Scholar]
  3. Bizzozero G. 1893; Ueber die schlauchförmigen Drüsen des magendarmkanals und die Beziehungen ihres Epithels zu dem Oberflächenepithel der Schleimhaut. Arch Mikrosk Anat 42:82
    [Google Scholar]
  4. Blaser M. J., Perez-Perez G. L, Lindenbaum J., Schneidman D., Van Deventer G., Marin-Sorensen M., Weinstein W. M. 1991; Association of infection due to Helicobacter pylori with specific upper gastrointestinal pathology. Rev Infect Dis 13:suppl. 8S704–S708
    [Google Scholar]
  5. Boom R., Sol C. J. A., Salimans M. M. M., Jansen C. L., Wertheim-van Dillen P. M. E., van der Noordaa J. 1990; Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28:495–503
    [Google Scholar]
  6. Cover T. L., Blaser M. J. 1995; Helicobacter pylori’, a bacterial cause of gastritis, peptic ulcer disease, and gastric cancer. ASM News 61:21–26
    [Google Scholar]
  7. Dent J. G, McNulty C. A. M., Uff J. G, Wilkinson S. P., Gear M. W. L. 1987; Spiral organisms in the gastric antrum [letter]. Lancet ii:96
    [Google Scholar]
  8. Dewhirst F. E., Seymour G, Fraser G. J., Paster B. J., Fox J. G. 1994; Phylogeny of Helicobacter isolates from bird and swine feces and description of Helicobacter pametensis sp. nov. Int J Syst Bacteriol 44:553–560
    [Google Scholar]
  9. Eaton K. A., Radin M. J., Kramer L., Wack R., Sherding R., Krakowka S., Fox J. G., Morgan D. R. 1993; Epizootic gastritis associated with gastric spiral bacilli in cheetahs (Acinonyx jubatus). Vet Pathol 30:55–63
    [Google Scholar]
  10. Franklin C. L., Beckwith C. S., Livingston R. S., Riley L. K., Gibson S. V., Besch-Williford C. L., Hook R. R. Jr 1996; Isolation of a novel Helicobacter species, Helicobacter cholecystus sp. nov., from the gallbladders of Syrian hamsters with cholangiofibrosis and centrilobular pancreatitis. J Clin Microbiol 34:2952–2958
    [Google Scholar]
  11. Hänninen M.-L., Happonen I., Saari S., Jalava K. 1996; Culture and characteristics of Helicobacter bizzozeronii. a new canine gastric Helicobacter sp. Int J Syst Bacteriol 46:160–166
    [Google Scholar]
  12. Jakob W., Stolte M., Valentin A., Schroder H. D. 1997; Demonstration of Helicobacter pylori-Wke organisms in the gastric mucosa of captive exotic carnivores. J Comp Pathol 116:21–33
    [Google Scholar]
  13. Jalava K., Kaartinen M., Utriainen M., Happonen I., Hänninen M.-L. 1997; Helicobacter salomonis sp. nov., a canine gastric Helicobacter sp. related to Helicobacter felis and Helicobacter bizzozeronii. Int J Syst Bacteriol 47:975–982
    [Google Scholar]
  14. Lapage S. P., Sneath P. H. A., Lessel E. F., Skerman V. B. D., Seeliger H. P. R., Clark W. A.editors 1992 International Code of Nomenclature of Bacteria, 1990 Revision. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Lee A., Hazell S. L., O’Rourke J., Kouprach S. 1988; Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun 56:2843–2850
    [Google Scholar]
  16. McNulty C. A., Dent J. G, Curry A., Uff J. S., Ford G. A., Gear M. W., Wilkinson S. P. 1989; New spiral bacterium in gastric mucosa. J Clin Pathol 42:585–591
    [Google Scholar]
  17. Marshall B. J., Warren J. R. 1984; Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet i:1311–1315
    [Google Scholar]
  18. Mendes E. N., Queiroz D. M. M., Rocha G. A., Moura S. B., Leite V. H. R., Fonseca M. E. F. 1990; Ultrastructure of a spiral micro-organism from pig gastric mucosa (“ Gastrospirillum siiis’). J Med Microbiol 33:61–66
    [Google Scholar]
  19. Mendes E. N., Queiroz D. M. M., Rocha G. A., Nogueira A. M., Carvalho A. C., Lage A. P., Barbosa A. J. 1991; Histopathological study of porcine gastric mucosa with and without a spiral bacterium (‘ Gastrospirillum suis’). J Med Microbiol 35:345–348
    [Google Scholar]
  20. Mendes E. N., Queiroz D. M. M., Dewhirst F. E., Paster B. J., Rocha G. A., Fox J. G. 1994; Are pigs a reservoir host for human Helicobacter infection? (abstract). Am J Gastroenterol 89:1296
    [Google Scholar]
  21. Mendes E. N., Queiroz D. M. M., Coimbra R. S., Moura S. B., Barbosa A. J., Rocha G. A. 1996a; Experimental infection of Wistar rats with ‘Gastrospirillum suis’. J Med Microbiol 44:105–109
    [Google Scholar]
  22. Mendes E. N., Queiroz D. M. M., Dewhirst F. E., Paster B. J., Moura S. B., Fox J. G. 1996b; Helicobacter trogontum sp. nov., isolated from the rat intestine. Int J Syst Bacteriol 46:916–921
    [Google Scholar]
  23. Moura S. B., Queiroz D. M., Mendes E. N., Nogueira A. M., Rocha G. A. 1993; The inflammatory response of the gastric mucosa of mice experimentally infected with ‘Gastrospirillum suis’. J Med Microbiol 39:64–68
    [Google Scholar]
  24. Murray R. G. E., Schleifer K. H. 1994; Taxonomic notes: a proposal for recording the properties of putative taxa of procaryotes. Int J Syst Bacteriol 44:174–176
    [Google Scholar]
  25. Murray R. G. E., Stackebrandt E. 1995; Taxonomic note: implementation of the provisional status Candidatus for incompletely described procaryotes. Int J Syst Bacteriol 45:186–187
    [Google Scholar]
  26. On S. L. 1996; Identification methods for Campylobacters, helicobacters, and related organisms. Clin Microbiol Rev 9:405–422
    [Google Scholar]
  27. On S. L. W., Bloch B., Holmes B., Hoste B., Vandamme P. 1995; Campylobacter hyointestinalis subsp. lawsonii subsp. nov., isolated from the porcine stomach, and an emended description of Campylobacter hyointestinalis. Int J Syst Bacteriol 45:767–774
    [Google Scholar]
  28. O’Rourke J., Solnick J., Lee A., Tompkins L. 1992; Helicobacter heilmannii (previously Gastrospirillum), a new species of Helicobacter in humans and animals [abstract]. Ir J Med Sci 161:suppl. 1031
    [Google Scholar]
  29. Parsonnet J., Friedman G. D., Vandersteen D. P., Chang Y., Vogelman J. H., Orentreich N., Sibley R. K. 1991; Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med 325:1127–1131
    [Google Scholar]
  30. Paster B. J., Lee A., Fox J. G., Dewhirst F. E., Tordoff L. A., Fraser G. J., O’Rourke J. L., Taylor N. S., Ferrero R. 1991; Phylogeny of Helicobacter felis sp. nov., Helicobacter mustelae, and related bacteria. Int J Syst Bacteriol 41:31–38
    [Google Scholar]
  31. Queiroz D. M. M., Rocha G. A., Mendes E. N., Lage A. P., Carvalho A. C. T., Barbosa A. J. A. 1990; A spiral microorganism in the stomach of pigs. Vet Microbiol 24:199–204
    [Google Scholar]
  32. Queiroz D. M. M., Rocha G. A., Mendes E. N., De Moura S. B., De Oliveira A. M., Miranda D. 1996; Association between Helicobacter and gastric ulcer disease of the pars esophagea in swine. Gastroenterology 111:19–27
    [Google Scholar]
  33. Rappin J. 1881 Contribution à l’étude de bactéries de la bouche à l’état normal PhD thesis Collège de France; Nantes, France:
    [Google Scholar]
  34. Seymour C., Lewis R. G., Kim M., Gagnon D. F., Fox J. G., Dewhirst F. E., Paster B. J. 1994; Isolation of Helicobacter strains from wild bird and swine feces. Appl Environ Microbiol 60:1025–1028
    [Google Scholar]
  35. Shen Z., Fox J. G., Dewhirst F. E., Paster B. J., Foltz C. J., Yan L., Shames B., Perry L. 1997; Helicobacter rodentium sp. nov., a urease-negative Helicobacter species isolated from laboratory mice. Int J Syst Bacteriol 47:627–634
    [Google Scholar]
  36. Solnick J. V., O’Rourke J., Lee A., Paster B. J., Dewhirst F. E., Tompkins L. S. 1993; An uncultured gastric spiral organism is a newly identified Helicobacter in humans. J Infect Dis 168:379–385
    [Google Scholar]
  37. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849
    [Google Scholar]
  38. Triiper H. G., De’Clari L. 1997; Taxonomic note: necessary correction of specific epithets formed as substantives (nouns) ‘in apposition’. Int J Syst Bacteriol 47:908–909
    [Google Scholar]
  39. Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. 1991; Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol 41:88–103
    [Google Scholar]
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