1887

Abstract

An improved pulsed-field electrophoresis program was developed to study differently sized chromosomes within the genus The number of chromosomes in the type strains was shown to be nine in and , 12 in and , 16 in and seven in The sizes of individual chromosomes were resolved and the approximate genome sizes were determined by the addition of individual chromosomes of the karyotypes. Apparently, the genome of , which is the only yeast to contain small chromosomes, is larger than that of On the other hand, other species exhibited genome sizes that were 10–25% smaller than that of Well-defined karyotypes represent the basis for future genome mapping and sequencing projects, as well as studies of the origin of the modern genomes.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/00207713-49-4-1925
1999-10-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/49/4/ijs-49-4-1925.html?itemId=/content/journal/ijsem/10.1099/00207713-49-4-1925&mimeType=html&fmt=ahah

References

  1. Cardinali G., Martini A. 1994; Electrophoretic karyotypes of authentic strains of the sensu stricto group of the genus Saccharomyces. Int J Sy st Bacteriol 44:791–797
    [Google Scholar]
  2. Carle G. F., Olson M. V. 1985; An electrophoretic karyotype for yeast. Proc Natl Acad Sci USA 82:3756–3760
    [Google Scholar]
  3. Gerring S. L, Connelly C., Hieter P. 1991; Positional mapping of genes by chromosome blotting and chromosome fragmentation. Methods Enzymol 194:57–77
    [Google Scholar]
  4. Goffeau A., Barrell B. G., Bussey H.13 other authors 1996; Life with 6000 geneS. Science 274:546–567
    [Google Scholar]
  5. Groth C. 1998 Saccharomyces sensu stricto yeasts: characterization of mitochondrial DNA MSc thesis University of Copenhagen; Copenhagen, Denmark:
    [Google Scholar]
  6. Jäger D., Philippsen P. 1989; Many yeast chromosomes lack the telomere-specific Y’ sequence. Mol Cell Biol 9:5754–5757
    [Google Scholar]
  7. James S. A., Cai J., Roberts I. N., Collins M. D. 1997; A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences: description of Saccharomyces kuna-shirensis sp. nov. and Saccharomyces martiniae sp. nov. Int J Syst Bacteriol 47:453–460
    [Google Scholar]
  8. Johnston J. R., Mortimer R. K. 1986; Electrophoretic karyotyping of laboratory and commercial strains of Saccharomyces and other yeastS. Int J Syst Bacteriol 36:569–572
    [Google Scholar]
  9. de Jonge P., de Jongh F. C. M., Meijers R., Steensma H. Y., Scheffers W. A. 1986; Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeastS. Yeast 2:193–204
    [Google Scholar]
  10. Keogh R. S., Seoighe C., Wolfe K. H. 1998; Evolution of gene order and chromosome number in Saccharomyces, Kluyveromyces and related fungi. Yeast 14:443–457
    [Google Scholar]
  11. Kurtzman C. P. 1993; Systematics of the ascomycetous yeasts assessed from ribosomal RNA sequence divergence. Antonie Leeuwenhoek 63:165–174
    [Google Scholar]
  12. Kurtzman C. P., Robnett C. J. 1991; Phylogenetic relationships among species of Saccharomyces, Schizosaccharomyces, Debaryomyces and Schwanniomyces determined from partial ribosomal RNA sequenceS. Yeast 7:61–72
    [Google Scholar]
  13. Naumov G. I., Naumova E. S., Lantto R. A., Louis E. J., Korhola M. 1992; Genetic homology between Saccharomyces cerevisiae and its sibling species S. paradoxus and S. bayanus: electrophoretic karyotypeS. Yeast 8:599–612
    [Google Scholar]
  14. Naumov G. I., Naumova E. S., Korhola M. 1995; Karyotypic relationships among species of Saccharomyces sensu lato: S’. castellii, S. dairensis, S. unisporus and Sservazzii. Syst Appl Microbiol 18:103–108
    [Google Scholar]
  15. Naumova E. S., Naumov G. I., Korhola M. 1996; Use of molecular karyotyping for differentiation of species in the heterogeneous taxon Saccharomyces exiguus. J Gen Appl Microbiol 42:307–314
    [Google Scholar]
  16. Nilsson-Tillgren T., Kielland-Brandt M. C., Holmberg S., Petersen J. G. L., Gjermansen C. 1983 Is lager yeast a species hybrid? Utilization of intrinsic genetic variation in breeding. Genetics of Industrial Microorganisms143–147 Edited by Ikeda Y., Beppu T. Tokyo: Kodanska;
    [Google Scholar]
  17. Oda Y., Tonomura K. 1995; Electrophoretic karyotyping of the yeast genus Torulaspora. Lett Appl Microbiol 21:190–193
    [Google Scholar]
  18. Oda Y., Yabuki M., Tonomura K., Fukunaga M. 1997; A phylogenetic analysis of Saccharomyces species by the sequence of 18S-28S rRNA spacer regionS. Yeast 13:1243–1250
    [Google Scholar]
  19. Pedersen M. B. 1986a; DNA sequence polymorphisms in the genus Saccharomyces. III. Restriction endonuclease fragment patterns of chromosomal regions in brewing and other yeast strainS. Carlsberg Res Commun 51:163–183
    [Google Scholar]
  20. Pedersen M. B. 1986b; DNA sequence polymorphisms in the genus Saccharomyces. IV. Homoeologous chromosomes III of Saccharomyces bay anus, S. carlsbergensis, and S. uvarum. Carlsberg Res Commun 51:185–202
    [Google Scholar]
  21. Petersen R. F. 1998 Genome dynamics and evolution of the mitochondrial and nuclear genomes in Saccharomyces sensu lato species PhD thesis University of Copenhagen; Copenhagen, Denmark:
    [Google Scholar]
  22. Peterson S. W., Kurtzman G P. 1991; Ribosomal RNA sequence divergence among sibling species of yeastS. Syst Appl Microbiol 14:124–129
    [Google Scholar]
  23. PiSkur J., Smole S., Groth G, Petersen R. F., Pedersen M. B. 1998; Structure and genetic stability of mitochondrial genomes vary among yeasts of the genus Saccharomyces. Int J Syst Bacteriol 48:1015–1024
    [Google Scholar]
  24. Sor F., Fukuhara H. 1989; Analysis of chromosomal DNA patterns of the genus Kluyveromyces. Yeast 5:1–10
    [Google Scholar]
  25. Vaughan Martini A., Kurtzman G P. 1985; Deoxyribonucleic acid relatedness among species of the genus Saccharomyces sensu stricto. Int J Syst Bacteriol 35:508–511
    [Google Scholar]
  26. Vaughan-Martini A., Martini A. 1987; Three newly delimited species of Saccharomyces sensu stricto. Antonie Leeuwenhoek 53:77–84
    [Google Scholar]
  27. Vaughan-Martini A., Martini A., Cardinal) G. 1993; Electrophoretic karyotyping as a taxonomic tool in the genus Saccharomyces. Antonie Leeuwenhoek 63:145–156
    [Google Scholar]
  28. Wolfe K. H., Shields D. C. 1997; Molecular evidence for an ancient duplication of the entire yeast genome. Nature 387:708–713
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/00207713-49-4-1925
Loading
/content/journal/ijsem/10.1099/00207713-49-4-1925
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error