RT Journal Article SR Electronic(1) A1 Roos, S A1 Karner, F A1 Axelsson, L A1 Jonsson, HYR 2000 T1 Lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine. JF International Journal of Systematic and Evolutionary Microbiology, VO 50 IS 1 SP 251 OP 258 DO https://doi.org/10.1099/00207713-50-1-251 PB Microbiology Society, SN 1466-5034, AB A new Lactobacillus species from pig small intestine has been identified. In an attempt to isolate Lactobacillus reuteri strains carrying the putative colonization-factor gene (mub, for mucus binding) a mub-derived gene probe was used to screen pig intestinal material. A number of isolates were obtained and primary characterization showed that they were Gram-positive, catalase-negative, non-spore-forming, non-motile rods. Growth occurred at 45 degrees C but not at 15 degrees C and the DNA G+C content was 46 mol%. Cell wall analysis together with DNA-DNA hybridization and analysis of the 16S rRNA sequence revealed that the new isolates represent a previously undescribed Lactobacillus species closely related to L. reuteri, Lactobacillus fermentum and Lactobacillus pontis. The name Lactobacillus mucosae is proposed for this species and the type strain is S32T., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-50-1-251