- International Journal of Systematic and Evolutionary Microbiology
- Volume 50, Issue 3
f Clostridium peptidivorans sp. nov., a peptide-fermenting bacterium from an olive mill wastewater treatment digester.
- Authors: T Mechichi, M L Fardeau, M Labat, J L Garcia, F Verhé, B K Patel
- First Published Online: 01 May 2000, International Journal of Systematic and Evolutionary Microbiology 50: 1259-1264, doi: 10.1099/00207713-50-3-1259
- Subject: Articles
- Issue Published:
A new peptide-degrading, strictly anaerobic bacterium, designated strain TMC4T, was isolated from an olive mill wastewater treatment digester. Cells of strain TMC4T were motile, rod-shaped (5-10 x 0.6-1.2 microm), stained Gram-positive and formed terminal to subterminal spores that distended the cells. Optimal growth occurred at 37 degrees C and pH 7 in an anaerobic basal medium containing 0.5% Casamino acids. Arginine, lysine, cysteine, methionine, histidine, serine, isoleucine, yeast extract, peptone, Biotrypcase, gelatin and crotonate also supported growth, but not carbohydrates, organic acids or alcohols. The end-products of degradation were: acetate and butyrate from lysine and crotonate; acetate, butyrate, H2 and CO2 from Biotrypcase, gelatin and peptone; acetate, alanine, H2 and CO2 from cysteine; acetate, H2 and CO2 from serine, cysteine and yeast extract; acetate and formate from histidine; propionate from methionine; methyl 2-butyrate, H2 and CO2 from isoleucine; acetate and ethanol from arginine; and acetate, propionate, butyrate, methyl 2-butyrate, H2 and CO2 from Casamino acids. The DNA G+C content of strain TMC4T was 31 mol%. Phylogeny based on 16S rRNA sequence analysis showed that strain TMC4T was a member of the low-G+C-content Gram-positive genus Clostridium, with the closest relative being Clostridium pascui (sequence similarity of 96 %). Due to considerable differences in genomic and phenotypic properties between strain TMC4T and those of its nearest relative, strain TMC4T is proposed as a new species of the genus Clostridium, Clostridium peptidivorans sp. nov. Strain TMC4T has been deposited in the DSMZ as strain DSM 12505T.
Article metrics loading...
Full text loading...
Author and Article Information
/content/journal/ijsem/10.1099/00207713-50-3-1259dcterms_title,dcterms_subject,pub_serialTitlepub_serialIdent:journal/ijsem AND -contentType:BlogPost104
/content/journal/ijsem/10.1099/00207713-50-3-1259dcterms_title,dcterms_subject-pub_serialIdent:journal/ijsem AND -contentType:BlogPost104
Figure data loading....