RT Journal Article SR Electronic(1) A1 Ivanova, Elena P A1 Gorshkova, Nataliya M A1 Sawabe, Tomoo A1 Hayashi, Karin A1 Kalinovskaya, Nataliya I A1 Lysenko, Anatolii M A1 Zhukova, Natalie V A1 Nicolau, Dan V A1 Kuznetsova, Tatyana A A1 Mikhailov, Valery V A1 Christen, RichardYR 2002 T1 Pseudomonas extremorientalis sp. nov., isolated from a drinking water reservoir. JF International Journal of Systematic and Evolutionary Microbiology, VO 52 IS 6 SP 2113 OP 2120 DO https://doi.org/10.1099/00207713-52-6-2113 PB Microbiology Society, SN 1466-5034, AB On the basis of phenotypic and genotypic characteristics and 16S rDNA sequence analysis, a novel species belonging to the genus Pseudomonas sensu stricto was identified. The saprophytic, fluorescent bacterium, designated KMM 3447(T), was isolated from a drinking water reservoir near Vladivostok City, Russia. The novel organism was a Gram-negative, aerobic, rod-shaped bacterium that produced a cyclic depsipeptide with surface-active properties. It degraded casein, but did not degrade gelatin, starch, agar or Tween 80. The bacterium was also haemolytic. Growth of the novel bacterium occurred between 4 and 35 degrees C. The predominant cellular fatty acids of the novel pseudomonad were C16:0, C16:1(n-7), C18:1(n-7) and C17.0 cyclo; branched fatty acids were only found in trace amounts. The G+C content of the novel bacterium was 61.0 mol%. 16S rDNA sequence analysis indicated that the novel bacterium had a clear affiliation with Pseudomonas fluorescens and species closely related to this recognized pseudomonad. DNA-DNA hybridization experiments showed that the novel bacterium bound at low levels (27-53%) with the DNA of the type strains of its nearest phylogenetic relatives, namely Pseudomonas tolaasii, Pseudomonas veronii, Pseudomonas orientalis and Pseudomonas rhodesiae, indicating that the novel bacterium represented a novel species within the genus Pseudomonas, for which the name Pseudomonas extremorientalis is proposed; the type strain is KMM 3447(T) (= LMG 19695(T))., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-52-6-2113