1887

Abstract

A novel hyperthermophilic, anaerobic, archaeon was isolated from a terrestrial hot spring at Uzon Caldera, Kronotsky Nature Reserve, Kamchatka, Russia. The isolate, strain 1860, grew optimally at 90–95 °C and pH 6.0–7.0. The cells were non-motile straight rods, 1.5–5.0 µm in length, covered with surface-layer lattice. Strain 1860 utilized complex proteinaceous compounds as electron donors and ferrihydrite, Fe(III) citrate, nitrate, thiosulfate, selenite, selenate and arsenate as electron acceptors for growth. The sequence of the 16S rRNA gene of strain 1860 had 97.9–98.7 % similarity with those of members of the genus . On the basis of its physiological properties and phylogenetic analyses including genome to genome hybridization, the isolate is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is 1860 ( = DSM 28942 = VKM B-2856).

Funding
This study was supported by the:
  • Russian Foundation for Basic Research (Award 13-04-40205 N)
  • Russian Science Foundation (Award 14-24-00165)
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/content/journal/ijsem/10.1099/ijs.0.000027
2015-03-01
2024-04-23
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403410. [View Article] [PubMed]
    [Google Scholar]
  2. Amo T., Paje M. L. F., Inagaki A., Ezaki S., Atomi H., Imanaka T. ( 2002 ). Pyrobaculum calidifontis sp. nov., a novel hyperthermophilic archaeon that grows in atmospheric air. . Archaea 1, 113121. [View Article] [PubMed]
    [Google Scholar]
  3. Benson D. A., Boguski M. S., Lipman D. J., Ostell J., Ouellette B. F., Rapp B. A., Wheeler D. L. ( 1999 ). GenBank. . Nucleic Acids Res 27, 1217. [View Article] [PubMed]
    [Google Scholar]
  4. Chan P. P., Cozen A. E., Lowe T. M. ( 2013 ). Reclassification of Thermoproteus neutrophilus Stetter and Zillig 1989 as Pyrobaculum neutrophilum comb. nov. based on phylogenetic analysis. . Int J Syst Evol Microbiol 63, 751754. [View Article] [PubMed]
    [Google Scholar]
  5. Feinberg L. F., Srikanth R., Vachet R. W., Holden J. F. ( 2008 ). Constraints on anaerobic respiration in the hyperthermophilic Archaea Pyrobaculum islandicum and Pyrobaculum aerophilum . . Appl Environ Microbiol 74, 396402. [View Article] [PubMed]
    [Google Scholar]
  6. Fischer F., Zillig W., Stetter K. O., Schreiber G. ( 1983 ). Chemolithoautotrophic metabolism of anaerobic extremely thermophilic archaebacteria. . Nature 301, 511513. [View Article] [PubMed]
    [Google Scholar]
  7. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007 ). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. . Int J Syst Evol Microbiol 57, 8191. [View Article] [PubMed]
    [Google Scholar]
  8. Huber R., Kristjansson J. K., Stetter K. O. ( 1987 ). Pyrobaculum gen. nov., a new genus of neutrophilic, rod-shaped archaebacteria from continental solfataras growing optimally at 100°C. . Arch Microbiol 149, 95101. [View Article]
    [Google Scholar]
  9. Huber R., Huber H., Stetter K. O. ( 2000 ). Towards the ecology of hyperthermophiles: biotopes, new isolation strategies and novel metabolic properties. . FEMS Microbiol Rev 24, 615623. [View Article] [PubMed]
    [Google Scholar]
  10. Itoh T., Nomura N., Sako Y. ( 2003 ). Distribution of 16S rRNA introns among the family Thermoproteaceae and their evolutionary implications. . Extremophiles 7, 229233.[PubMed]
    [Google Scholar]
  11. Mardanov A. V., Gumerov V. M., Slobodkina G. B., Beletsky A. V., Bonch-Osmolovskaya E. A., Ravin N. V., Skryabin K. G. ( 2012 ). Complete genome sequence of strain 1860, a crenarchaeon of the genus Pyrobaculum able to grow with various electron acceptors. . J Bacteriol 194, 727728. [View Article] [PubMed]
    [Google Scholar]
  12. Meier-Kolthoff J. P., Auch A. F., Klenk H.-P., Göker M. ( 2013 ). Genome sequence-based species delimitation with confidence intervals and improved distance functions. . BMC Bioinformatics 14, 60. [View Article] [PubMed]
    [Google Scholar]
  13. Richter M., Rosselló-Móra R. ( 2009 ). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106, 1912619131. [View Article] [PubMed]
    [Google Scholar]
  14. Sako Y., Nunoura T., Uchida A. ( 2001 ). Pyrobaculum oguniense sp. nov., a novel facultatively aerobic and hyperthermophilic archaeon growing at up to 97 degrees C. . Int J Syst Evol Microbiol 51, 303309.[PubMed]
    [Google Scholar]
  15. Salman V., Amann R., Shub D. A., Schulz-Vogt H. N. ( 2012 ). Multiple self-splicing introns in the 16S rRNA genes of giant sulfur bacteria. . Proc Natl Acad Sci U S A 109, 42034208. [View Article] [PubMed]
    [Google Scholar]
  16. Slobodkin A., Reysenbach A.-L., Strutz N., Dreier M., Wiegel J. ( 1997 ). Thermoterrabacterium ferrireducens gen. nov., sp. nov., a thermophilic anaerobic dissimilatory Fe(III)-reducing bacterium from a continental hot spring. . Int J Syst Bacteriol 47, 541547. [View Article] [PubMed]
    [Google Scholar]
  17. Slobodkin A. I., Tourova T. P., Kuznetsov B. B., Kostrikina N. A., Chernyh N. A., Bonch-Osmolovskaya E. A. ( 1999 ). Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium. . Int J Syst Bacteriol 49, 14711478. [View Article] [PubMed]
    [Google Scholar]
  18. Tamura K., Nei M. ( 1993 ). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. . Mol Biol Evol 10, 512526.[PubMed]
    [Google Scholar]
  19. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013 ). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30, 27252729. [View Article] [PubMed]
    [Google Scholar]
  20. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P. ( 2010 ). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60, 249266. [View Article] [PubMed]
    [Google Scholar]
  21. Trueper H. G., Schlegel H. G. ( 1964 ). Sulfur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells of Chromatium okenii . . Antonie van Leeuwenhoek 30, 225238. [View Article] [PubMed]
    [Google Scholar]
  22. Vargas M., Kashefi K., Blunt-Harris E. L., Lovley D. R. ( 1998 ). Microbiological evidence for Fe(III) reduction on early Earth. . Nature 395, 6567. [View Article] [PubMed]
    [Google Scholar]
  23. Völkl P., Huber R., Drobner E., Rachel R., Burggraf S., Trincone A., Stetter K. O. ( 1993 ). Pyrobaculum aerophilum sp. nov., a novel nitrate-reducing hyperthermophilic archaeum. . Appl Environ Microbiol 59, 29182926.[PubMed]
    [Google Scholar]
  24. Wolin E. A., Wolin M. J., Wolfe R. S. ( 1963 ). Formation of methane by bacterial extracts. . J Biol Chem 238, 28822886.[PubMed]
    [Google Scholar]
  25. Yokobori S., Itoh T., Yoshinari S., Nomura N., Sako Y., Yamagishi A., Oshima T., Kita K., Watanabe Y. ( 2009 ). Gain and loss of an intron in a protein-coding gene in Archaea: the case of an archaeal RNA pseudouridine synthase gene. . BMC Evol Biol 9, 198. [View Article] [PubMed]
    [Google Scholar]
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