RT Journal Article SR Electronic(1) A1 Horino, Haruka A1 Ito, Miyuki A1 Tonouchi, AkioYR 2015 T1 Clostridium oryzae sp. nov., from soil of a Japanese rice field JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_3 SP 943 OP 951 DO https://doi.org/10.1099/ijs.0.000042 PB Microbiology Society, SN 1466-5034, AB An obligately anaerobic bacterial strain designated KC3T was isolated from a rice straw-degrading culture, for which soil of a Japanese rice field was used as the inoculum. Cells of strain KC3T were determined to be non-cellulolytic, Gram-stain-positive, non-motile, ellipsoidal, spore-forming rods, 0.8–1×4–25 µm. Endospores were formed at a terminal position in elongated cells (12–25 µm, mean 15 µm). The temperature range for growth was 20–50 °C, with an optimum at 37 °C. The pH range for growth was 5.0–7.5, with an optimum at pH 6.0 (slightly acidophilic). Strain KC3T fermented cellobiose to lactate, butyrate, acetate, formate, hydrogen and carbon dioxide. The major cellular fatty acids (>10 %) were C14 : 0, C16 : 0 and C19 : 0 cyclo 11,12 dimethylacetal. The DNA G+C content of strain KC3T was 37.5 mol%. 16S rRNA gene sequence analysis revealed that strain KC3T shared low sequence similarity (<93 %) with type strains of the genus Clostridium sensu stricto (Clostridium rRNA cluster I). Analyses of the DNA gyrase A and ATP synthase beta subunit sequences supported the affiliation of strain KC3T to the genus Clostridium sensu stricto. The evidence presented here indicates that strain KC3T represents a novel species of the genus Clostridium , for which the name Clostridium oryzae sp. nov. is proposed. The type strain of Clostridium oryzae is KC3T ( = DSM 28571T = NBRC 110163T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000042