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Volume 65, Issue Pt_3

sp. nov., a methanogen isolated from deep marine sediment at the deformation front area near Taiwan Free

Abstract

A mesophilic, hydrogenotrophic methanogen, strain CYW4, was isolated from deep-sea sediment obtained by the Ocean Researcher I cruiser, ORI-961, in 2011. The sediment was from the deformation front area offshore of south-western Taiwan. Here, seismic reflections indicated that methane hydrates were abundant. The methanogenic substrates utilized by strain CYW4 were formate and H/CO, but not acetate, secondary alcohols, methylamines, methanol and ethanol. Cells of strain CYW4 were non-motile, irregular cocci and 0.6–1.5 µm in diameter. The S-layer protein had an of 112 000. The optimum growth conditions were at 37 °C, pH 8.1 and 0.08 M NaCl. Growth of the strain was stimulated by acetate. The G+C content of the chromosomal DNA of strain CYW4 was 61 mol%. Phylogenetic analysis revealed that strain CYW4 was most closely related to JR1 (96.82 % 16S rRNA gene sequence similarity). Based on the morphological, phenotypic and phylogenetic characteristics presented here, it is evident that strain CYW4 represents a novel species of the genus , and the name sp. nov. is proposed. The type strain is CYW4 ( = BCRC AR10043 = NBRC 110782). The optical density of cultures of strain CYW4 dropped abruptly upon entering the stationary growth phase. During this time numerous particles of approximately 50 nm in diameter were observed on and around the cells. This suggests that strain CYW4 harbours a lytic virus that is induced in the stationary phase, which is of interest because only a few lytic viruses have been reported in methanogens.

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Funding
This study was supported by the:
  • Ministry of Education, Taiwan, R.O.C. under the ATU plan
  • Central Geological Survey, MOEA (Award 100-5226904000- 02-03)
  • National Science Council (Award NSC102-3113-P-005-002)
  • Ministry of Science and Technology Taiwan, R.O.C (Award MOST103-3113 M-005-001)
© 2015 IUMS
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2015-03-01
2024-04-19
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Methanoculleus taiwanensis sp. nov., a methanogen isolated from deep marine sediment at the deformation front area near Taiwan
Int J Syst Evol Microbiol 65, 1044 (2015); https://doi.org/10.1099/ijs.0.000062
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