1887

Abstract

A Gram-stain-negative, non-motile, rod-shaped bacterial strain, L-1, which was capable of degrading methyl red was isolated from a dye-manufacturing factory in China. Phenotypic, chemotaxonomic and phylogenetic analyses established affiliation of the isolate to the genus . Cells occurred in pairs in young cultures but became chains of coccoid cells in old cultures, and produced a flexirubin-like yellow pigment. Strain L-1 could not hydrolyse cellulose, and had a DNA G+C content of 51.3 mol%. The major cellular fatty acids were iso-C, Cω5, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6). C, iso-C 3-OH and C 3-OH were the other major fatty acid components. Comparative 16S rRNA gene sequence analysis showed that strainL-1 was most closely related to DSM 18053 (99.2 %), JCM 16232 (98.9 %) and CGMCC 1.6375 (98.7 %). However, the new isolate exhibited relatively low levels of DNA–DNA relatedness with respect to JCM 16232 (41.2±1.8 %), DSM 18053 (38.6±2.6 %) and CGMCC 1.6375 (35.0±2.1 %). Strain L-1 could also be differentiated from its closest phylogenetic relatives based on differences in several phenotypic characteristics. These data suggest that strain L-1 represents a novel species of the genus , for which the name sp. is proposed. The type strain is L-1 (DSM 29057 = CGMCC 1.12969).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 21107002)
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2015-04-01
2024-03-28
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References

  1. Baik K.-S., Kim M.-S., Kim E.-M., Kim H.-R., Seong C. N. ( 2007 ). Dyadobacter koreensis sp. nov., isolated from fresh water. . Int J Syst Evol Microbiol 57, 12271231. [View Article] [PubMed]
    [Google Scholar]
  2. Chelius M. K., Triplett E. W. ( 2000 ). Dyadobacter fermentans gen. nov., sp. nov., a novel gram-negative bacterium isolated from surface-sterilized Zea mays stems. . Int J Syst Evol Microbiol 50, 751758. [View Article] [PubMed]
    [Google Scholar]
  3. Chen L., Jiang F., Xiao M.-C., Dai J., Kan W.-J., Fang C.-X., Peng F. ( 2013 ). Dyadobacter arcticus sp. nov., isolated from Arctic soil. . Int J Syst Evol Microbiol 63, 16161620. [View Article] [PubMed]
    [Google Scholar]
  4. Chun J., Kang J.-Y., Joung Y., Kim H., Joh K., Jahng K. Y. ( 2013 ). Dyadobacter jejuensis sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 63, 17881792. [View Article] [PubMed]
    [Google Scholar]
  5. Dong Z., Guo X., Zhang X., Qiu F., Sun L., Gong H., Zhang F. ( 2007 ). Dyadobacter beijingensis sp. nov., isolated from the rhizosphere of turf grasses in China. . Int J Syst Evol Microbiol 57, 862865. [View Article] [PubMed]
    [Google Scholar]
  6. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  8. Jadhav S. B., Surwase S. N., Kalyani D. C., Gurav R. G., Jadhav J. P. ( 2012 ). Biodecolorization of azo dye Remazol orange by Pseudomonas aeruginosa BCH and toxicity (oxidative stress) reduction in Allium cepa root cells. . Appl Biochem Biotechnol 168, 13191334. [View Article] [PubMed]
    [Google Scholar]
  9. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721.[PubMed] [CrossRef]
    [Google Scholar]
  10. Lee M., Woo S.-G., Park J., Yoo S.-A. ( 2010 ). Dyadobacter soli sp. nov., a starch-degrading bacterium isolated from farm soil. . Int J Syst Evol Microbiol 60, 25772582. [View Article] [PubMed]
    [Google Scholar]
  11. Liu Q.-M., Im W.-T., Lee M., Yang D.-C., Lee S.-T. ( 2006 ). Dyadobacter ginsengisoli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 56, 19391944. [View Article] [PubMed]
    [Google Scholar]
  12. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by highperformance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  13. Moore D. D., Dowhan D. ( 1995 ). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 211. Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. . New York:: Wiley;.
    [Google Scholar]
  14. Reddy G. S. N., Garcia-Pichel F. ( 2005 ). Dyadobacter crusticola sp. nov., from biological soil crusts in the Colorado Plateau, USA, and an emended description of the genus Dyadobacter Chelius and Triplett 2000. . Int J Syst Evol Microbiol 55, 12951299. [View Article] [PubMed]
    [Google Scholar]
  15. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  16. Sambrook J., Russell D. W. ( 2001 ). Molecular Cloning: A Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
    [Google Scholar]
  17. Sasser M. ( 1990 ). Identification of bacteria through fatty acid analysis. . In Methods in Phytobacteriology, pp. 199204. Edited by Klement Z., Rudolph K., Sands D. C. . Budapest:: Akademiai Kaido;.
    [Google Scholar]
  18. Shen L., Liu Y.-Q., Yao T.-D., Wang N.-L., Xu B.-Q., Jiao N.-Z., Liu H.-C., Zhou Y.-G., Liu X.-B., Wang Y.-N. ( 2013 ). Dyadobacter tibetensis sp. nov., isolated from glacial ice core. . Int J Syst Evol Microbiol 63, 36363639. [View Article] [PubMed]
    [Google Scholar]
  19. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  20. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  21. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013 ). mega 6: Molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30, 27252729. [View Article] [PubMed]
    [Google Scholar]
  22. Tang Y.-L., Dai J., Zhang L., Mo Z.-Y., Wang Y., Li Y.-W., Ji S.-M., Fang C., Zheng C. ( 2009 ). Dyadobacter alkalitolerans sp. nov., isolated from desert sand. . Int J Syst Evol Microbiol 59, 6064. [View Article] [PubMed]
    [Google Scholar]
  23. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  24. Weeks O. B. ( 1981 ). Preliminary studies of the pigments of Flavobacterium breve NCTC 11099 and Flavobacterium odoratum NCTC 11036. . In The Flavobacterium-Cytophaga Group, pp. 108114. Edited by Reichenbach H., Weeks O. B. . Weinheim:: Gesellschaft für Biotechnologische Forschung;.
    [Google Scholar]
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