@article{mbs:/content/journal/ijsem/10.1099/ijs.0.000070, author = "Grosche, Ashley and Sekaran, Hema and Pérez-Rodríguez, Ileana and Starovoytov, Valentin and Vetriani, Costantino", title = "Cetia pacifica gen. nov., sp. nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_4", pages = "1144-1150", doi = "https://doi.org/10.1099/ijs.0.000070", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000070", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain TB-6T, was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at 9° N. The cells were Gram-staining-negative and rod-shaped with one or more polar flagella. Cell size was approximately 1–1.5 µm in length and 0.5 µm in width. Strain TB-6T grew between 45 and 70 °C (optimum 55–60 °C), 0 and 35 g NaCl l−1 (optimum 20–30 g l−1) and pH 4.5 and 7.5 (optimum pH 5.5–6.0). Generation time under optimal conditions was 2 h. Growth of strain TB-6T occurred with H2 as the energy source, CO2 as the carbon source and nitrate or sulfur as electron acceptors, with formation of ammonium or hydrogen sulfide, respectively. Acetate, (+)-d-glucose, Casamino acids, sucrose and yeast extract were not used as carbon and energy sources. Inhibition of growth occurred in the presence of lactate, peptone and tryptone under a H2/CO2 (80 : 20; 200 kPa) gas phase. Thiosulfate, sulfite, arsenate, selenate and oxygen were not used as electron acceptors. The G+C content of the genomic DNA was 36.8 mol%. Phylogenetic analysis of the 16S rRNA gene of strain TB-6T showed that this organism branched separately from the three most closely related genera, Caminibacter , Nautilia and Lebetimonas , within the family Nautiliaceae . Strain TB-6T contained several unique fatty acids in comparison with other members of the family Nautiliaceae . Based on experimental evidence, it is proposed that the organism represents a novel species and genus within the family Nautiliaceae , Cetia pacifica, gen. nov., sp. nov. The type strain is TB-6T ( = DSM 27783T = JCM 19563T).", }