Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov., two spore-forming lactic acid bacteria isolated from tree barks in Thailand
Thamacharoensuk, Tanatip and Kitahara, Maki and Ohkuma, Moriya and Thongchul, Nuttha and Tanasupawat, Somboon,, 65, 1220-1226 (2015),
doi = https://doi.org/10.1099/ijs.0.000084,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= Two Gram-stain-positive, endospore-forming lactic acid bacteria, designated BK92T and BK117-1T, were isolated from tree barks in Thailand. Cells were catalase-negative and facultatively anaerobic rods. 16S rRNA gene sequence analysis indicated that these strains belonged to the genus Sporolactobacillus . Strains BK92T and BK117-1T showed the highest 16S rRNA gene sequence similarity to Sporolactobacillus putidus QC81-06T with 97.7 % and 97.1 % similarity, respectively. Analysis of phylogenetic relationships based on 16S rRNA and gyrB gene sequencing revealed that the positions of strains BK92T and BK117-1T were clearly separated from all related species of the genus Sporolactobacillus . Strains BK92T and BK117-1T had low DNA–DNA relatedness between each other and also with S. putidus QC81-06T and Sporolactobacillus vineae SL153T. The DNA G+C content of strains BK92T and BK117-1T was 46.6 mol% and 47.4 mol%, respectively. The major fatty acids of strains BK92T and BK117-1T were anteiso-C17 : 0 and anteiso-C15 : 0. They contained meso-diaminopimelic acid in cell-wall peptidoglycan and had menaquinone with seven isoprene units (MK-7) as the predominant menaquinone. Based on evidence including phenotypic, genotypic and chemotaxonomic studies, strains BK92T and BK117-1T should be classified as representatives of novel species of the genus Sporolactobacillus , for which the names Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov. are proposed, respectively. The type strains are BK92T ( = JCM 19541T = LMG 28365T = PCU 336T = TISTR 2234T) and BK117-1T ( = JCM 19542T = LMG 28366T = PCU 337T = TISTR 2235T).,
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