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Abstract
An aerobic halotolerant restricted facultatively methylotrophic bacterium was isolated from a saline hot spring in Pamukkale, Turkey, and designated strain PK2T. The cells of this strain were Gram-stain-negative, asporogenous, motile short rods multiplying by binary fission. They utilized methanol, methylamine and mannitol as carbon and energy sources. The organism grew optimally at 30 °C in media containing 85 mM NaCl and at pH 7.5–8.0. C1 compounds were assimilated via the isocitrate-lyase-positive variant of the serine pathway. Poly-β-hydroxybutyrate and the compatible solute ectoine were found in the cells. The dominant phospholipids were phosphatidylethanolamine and phosphatidylmonomethylethanolamine. The major cellular fatty acids of methanol-grown cells were C18 : 1ω7 and C16 : 1ω7c. The main ubiquinone was Q-10. The DNA G+C content was 67.9 mol% (T m). The 16S rRNA gene sequence suggests that strain PK2T is affiliated with the order Rhizobiales within the class Alphaproteobacteria , being most closely related to Mesorhizobium gobiense CCBAU 83330T (94 % similarity). A novel genus and species, Methylobrevis pamukkalensis gen. nov., sp. nov., is proposed on the basis of phenotypic and genotypic data, with PK2T (VKM B-2849T = JCM 30229T) as the type strain.
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Funding
- Russian Science Foundation (Award 14-14-01045)
- Russian Foundation for Basic Research (Award 14-04-31352)