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Volume 65, Issue Pt_4

sp. nov., isolated from human faeces Free

Abstract

A bacterial strain, designated 157, isolated from human faeces was characterized by using a polyphasic taxonomic approach, which included analysis of physiological and biochemical features, cellular fatty acid profiles, menaquinone profiles and its phylogenetic position, based on 16S rRNA gene sequence analysis. The strain was obligately anaerobic, non-pigmented, non-spore-forming, non-motile, Gram-stain-negative rods. The isolate was able to grown on medium containing 20 % (w/v) bile. 16S rRNA gene sequence analysis showed that the strain was a member of the genus . Strain 157 was closely related to JCM 15724 (96 % sequence similarity). The results of gene sequence analysis indicated that strain 157 was different from JCM 15724, with a gene sequence similarity of 96.1 %. The major cellular fatty acids of strain 157 were anteiso-C, iso-C 3-OH, Cω9 and anteiso-C 3-OH. The major menaquinone of the isolate was MK-9. The DNA G+C content of strain 157 was 41.8 mol%. On the basis of these data, strain 157 represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is 157 ( = JCM 18682 = CCUG 66681).

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2015-04-01
2024-04-20
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References

  1. Eckburg P. B., Bik E. M., Bernstein C. N., Purdom E., Dethlefsen L., Sargent M., Gill S. R., Nelson K. E., Relman D. A. ( 2005 ). Diversity of the human intestinal microbial flora. . Science 308, 1635–1638. [View Article] [PubMed]
    [Google Scholar]
  2. Felsenstein J. ( 1985 ). Confidence limits of phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  3. Holdeman L. V., Cato E. P., Moore W. E. C. ( 1977 ). Anaerobe Laboratory Manual, , 4th edn.. Blacksburg, VA:: Virginia Polytechnic Institute and State University;.
     [Google Scholar]
  4. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  5. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  6. Kuykendall L. D., Roy M. A., O’neill J. J., Devine T. E. ( 1988 ). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradyrhizobium japonicum . . Int J Syst Bacteriol 38, 358–361. [View Article]
    [Google Scholar]
  7. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. ( 2007 ). Clustal W and Clustal X version 2.0. . Bioinformatics 23, 2947–2948. [View Article] [PubMed]
    [Google Scholar]
  8. Li E., Hamm C. M., Gulati A. S., Sartor R. B., Chen H., Wu X., Zhang T., Rohlf F. J., Zhu W. et al. ( 2012 ). Inflammatory bowel diseases phenotype, C. difficile and NOD2 genotype are associated with shifts in human ileum associated microbial composition. . PLoS ONE 7, e26284. [View Article] [PubMed]
    [Google Scholar]
  9. McClung L. S., Lindberg R. B. ( 1957 ). The study of obligately anaerobic bacteria. . In Mannual of Microbiological Methods, pp. 120–139. Edited by Pelczar M. J. et al. . New York:: McGraw-Hill;.
     [Google Scholar]
  10. Miller L. T. ( 1982 ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. . J Clin Microbiol 16, 584–586.[PubMed]
    [Google Scholar]
  11. Qin J., Li R., Raes J., Arumugam M., Burgdorf K. S., Manichanh C., Nielsen T., Pons N., Levenez F. et al. ( 2010 ). A human gut microbial gene catalogue established by metagenomic sequencing. . Nature 464, 59–65. [View Article] [PubMed]
    [Google Scholar]
  12. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  13. Sakamoto M., Benno Y. ( 2006 ). Reclassification of Bacteroides distasonis, Bacteroides goldsteinii and Bacteroides merdae as Parabacteroides distasonis gen. nov., comb. nov., Parabacteroides goldsteinii comb. nov. and Parabacteroides merdae comb. nov.. Int J Syst Evol Microbiol 56, 1599–1605. [View Article] [PubMed]
    [Google Scholar]
  14. Sakamoto M., Ohkuma M. ( 2010 ). Usefulness of the hsp60 gene for the identification and classification of Gram-negative anaerobic rods. . J Med Microbiol 59, 1293–1302. [View Article] [PubMed]
    [Google Scholar]
  15. Sakamoto M., Ohkuma M. ( 2011 ). Identification and classification of the genus Bacteroides by multilocus sequence analysis. . Microbiology 157, 3388–3397.[PubMed] [CrossRef]
    [Google Scholar]
  16. Sakamoto M., Suzuki M., Umeda M., Ishikawa I., Benno Y. ( 2002 ). Reclassification of Bacteroides forsythus (Tanner et al. 1986) as Tannerella forsythensis corrig., gen. nov., comb. nov.. Int J Syst Evol Microbiol 52, 841–849. [View Article] [PubMed]
    [Google Scholar]
  17. Sakamoto M., Huang Y., Umeda M., Ishikawa I., Benno Y. ( 2005 ). Prevotella multiformis sp. nov., isolated from human subgingival plaque. . Int J Syst Evol Microbiol 55, 815–819. [View Article] [PubMed]
    [Google Scholar]
  18. Sakamoto M., Suzuki N., Matsunaga N., Koshihara K., Seki M., Komiya H., Benno Y. ( 2009 ). Parabacteroides gordonii sp. nov., isolated from human blood cultures. . Int J Syst Evol Microbiol 59, 2843–2847. [View Article] [PubMed]
    [Google Scholar]
  19. Sakamoto M., Suzuki N., Benno Y. ( 2010 ). hsp60 and 16S rRNA gene sequence relationships among species of the genus Bacteroides with the finding that Bacteroides suis and Bacteroides tectus are heterotypic synonyms of Bacteroides pyogenes . . Int J Syst Evol Microbiol 60, 2984–2990. [View Article] [PubMed]
    [Google Scholar]
  20. Sakamoto M., Tanaka Y., Benno Y., Ohkuma M. ( 2014 ). Butyricimonas faecihominis sp. nov. and Butyricimonas paravirosa sp. nov., isolated from human faeces, and emended description of the genus Butyricimonas . . Int J Syst Evol Microbiol 64, 2992–2997. [View Article] [PubMed]
    [Google Scholar]
  21. Shah H. N. ( 1992 ). The genus Bacteroides and related taxa. . In The Prokaryotes, , 2nd edn., pp. 3593–3607. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;. [View Article]
    [Google Scholar]
  22. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
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Parabacteroides faecis sp. nov., isolated from human faeces
Int J Syst Evol Microbiol 65, 1342 (2015); https://doi.org/10.1099/ijs.0.000109
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