1887

Abstract

A Gram-negative, non-motile, rod-shaped bacterial strain, designated SMK-117, belonging to the , was isolated from a tidal flat of the Yellow Sea, Korea, and was subjected to a polyphasic taxonomic study. Strain SMK-117 grew optimally at pH 7.0–8.0 and 30 °C in the presence of 2 % (w/v) NaCl. Neighbour-joining and maximum-likelihood phylogenetic trees based on 16S rRNA gene sequences showed that strain SMK-117 clustered with HTCC2654, with which it exhibited a sequence similarity of 95.3 %. Strain SMK-117 contained Q-10 as the predominant ubiquinone and C 7 and 11-methyl C 7 as the major fatty acids. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, an unidentified aminolipid and two unidentified phospholipids. The DNA G+C content was 63.5 mol%. Strain SMK-117 was differentiated from members of the genera and on the basis of differences in the fatty acid and polar lipid profiles. The phenotypic, chemotaxonomic and phylogenetic data indicated that strain SMK-117 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain of is SMK-117 (=KCTC 22244 =CCUG 55760).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000109-0
2009-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/1/48.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000109-0&mimeType=html&fmt=ahah

References

  1. Baik, K. S., Seong, C. N., Kim, E. M., Yi, H., Bae, K. S. & Chun, J.(2005).Hahella ganghwensis sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 55, 681–684.[CrossRef] [Google Scholar]
  2. Baumann, P. & Baumann, L.(1981). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes. In The Prokaryotes, pp. 1302–1331. Edited by M. P. Starr, H. Stolp, H. G. Trüper, A. Balows & H. G. Schlegel. Berlin: Springer.
  3. Bruns, A., Rohde, M. & Berthe-Corti, L.(2001).Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef] [Google Scholar]
  4. Cho, J.-C. & Giovannoni, S. J.(2004).Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. Int J Syst Evol Microbiol 54, 1129–1136.[CrossRef] [Google Scholar]
  5. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  6. Gu, J., Guo, B., Wang, Y.-N., Yu, S.-L., Inamori, R., Qu, R., Ye, Y.-G. & Wu, X.-L.(2007).Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. Int J Syst Evol Microbiol 57, 157–160.[CrossRef] [Google Scholar]
  7. Jung, S.-Y., Oh, T.-K. & Yoon, J.-H.(2006).Colwellia aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56, 33–37.[CrossRef] [Google Scholar]
  8. Jung, S.-Y., Jung, Y.-T., Oh, T.-K. & Yoon, J.-H.(2007).Photobacterium lutimaris sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 57, 332–336.[CrossRef] [Google Scholar]
  9. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  10. Labrenz, M., Lawson, P. A., Tindall, B. J., Collins, M. D. & Hirsch, P.(2005).Roseisalinus antarcticus gen. nov., sp. nov., a novel aerobic bacteriochlorophyll a-producing α-proteobacterium isolated from hypersaline Ekho Lake, Antarctica. Int J Syst Evol Microbiol 55, 41–47.[CrossRef] [Google Scholar]
  11. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  12. Lee, K., Choo, Y.-J., Giovannoni, S. J. & Cho, J.-C.(2007).Maritimibacter alkaliphilus gen. nov., sp. nov., a genome-sequenced marine bacterium of the Roseobacter clade in the order Rhodobacterales. Int J Syst Evol Microbiol 57, 1653–1658.[CrossRef] [Google Scholar]
  13. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  14. Lin, K.-Y., Sheu, S.-Y., Chang, P.-S., Cho, J.-C. & Chen, W.-M.(2007).Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan. Int J Syst Evol Microbiol 57, 1625–1629.[CrossRef] [Google Scholar]
  15. Martínez-Cánovas, M. J., Quesada, E., Martínez-Checa, F., del Moral, A. & Béjar, V.(2004).Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the α-Proteobacteria. Int J Syst Evol Microbiol 54, 1735–1740.[CrossRef] [Google Scholar]
  16. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  17. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  18. Suzuki, T., Muroga, Y., Takahama, M. & Nishimura, Y.(1999).Roseivivax halodurans gen. nov., sp. nov. and Roseivivax halotolerans sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from a saline lake. Int J Syst Bacteriol 49, 629–634.[CrossRef] [Google Scholar]
  19. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  20. Yi, H., Chang, Y.-H., Oh, H. W., Bae, K. S. & Chun, J.(2003).Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments. Int J Syst Evol Microbiol 53, 1013–1018.[CrossRef] [Google Scholar]
  21. Yi, H., Bae, K. S. & Chun, J.(2004).Aestuariibacter salexigens gen. nov., sp. nov. and Aestuariibacter halophilus sp. nov., isolated from tidal flat sediment, and emended description of Alteromonas macleodii. Int J Syst Evol Microbiol 54, 571–576.[CrossRef] [Google Scholar]
  22. Yoon, J.-H., Kim, H., Kim, S.-B., Kim, H.-J., Kim, W. Y., Lee, S. T., Goodfellow, M. & Park, Y.-H.(1996). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46, 502–505.[CrossRef] [Google Scholar]
  23. Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rRNA gene sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
  24. Yoon, J.-H., Kang, K. H. & Park, Y.-H.(2003).Psychrobacter jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 53, 449–454.[CrossRef] [Google Scholar]
  25. Yoon, J.-H., Oh, T.-K. & Park, Y.-H.(2004).Kangiella koreensis gen. nov., sp. nov. and Kangiella aquimarina sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 54, 1829–1835.[CrossRef] [Google Scholar]
  26. Yoon, J.-H., Oh, T.-K. & Park, Y.-H.(2005).Erythrobacter seohaensis sp. nov. and Erythrobacter gaetbuli sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55, 71–75.[CrossRef] [Google Scholar]
  27. Yoon, J.-H., Kang, S.-J. & Oh, T.-K.(2007a).Donghicola eburneus gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57, 73–76.[CrossRef] [Google Scholar]
  28. Yoon, J.-H., Kang, S.-J., Lee, S.-Y. & Oh, T.-K.(2007b).Phaeobacter daeponensis sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 57, 856–861.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000109-0
Loading
/content/journal/ijsem/10.1099/ijs.0.000109-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 48 - 52

Transmission electron micrograph of cells of strain SMK-117 from exponentially growing cultures. Bar, 1 µm.



IMAGE
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error