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Volume 65, Issue Pt_5

gen. nov., sp. nov., a strictly anaerobic iron-reducing bacterium isolated from coal-contaminated soil Free

Abstract

A strictly anaerobic, mesophilic, iron-reducing bacterial strain, IRF19, was isolated from coal-contaminated soil in the Republic of Korea. IRF19 cells were straight, rod-shaped, Gram-staining-negative and motile by means of flagella. The optimum pH and temperature for their growth were determined to be pH 7.5–8.0 and 40 °C, while the optimum range was pH 6.5–10.0 and 20–45 °C, respectively. Strain IRF19 did not require NaCl for growth but it tolerated up to 2 % (w/v). Growth was observed with yeast extract, -glucose, -fructose, -ribose, -mannitol, -mannose, -serine, -alanine and -isoleucine. Fe(III), elemental sulfur, thiosulfate and sulfate were used as electron acceptors. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain IRF19 is affiliated to the family and is most closely related to Zn2 (93.5 % similarity), VNs68 (93.2 %) and B2-1 (92.3 %). The major cellular fatty acids of strain IRF19 were C, iso-C and C, and the profile was distinct from those of the closely related species. The major respiratory quinone of strain IRF19 was menaquinone MK-5 (V-H). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown phospholipid and two unknown polar lipids. The G+C content of the genomic DNA of strain IRF19 was determined to be 37.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic results, strain IRF19 is considered to represent a novel species of a novel genus of the family , for which we propose the name gen. nov., sp. nov., with the type strain IRF19 ( = KCTC 15396 = JCM 19988).

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Funding
This study was supported by the:
  • National Research Foundation (NRF)
  • Ministry of Education, Science and Technology (MEST) (Award NRF-2013R1A2A2A05006754)
  • Korea Institute of Energy Technology Evaluation and Planning (KETEP) (Award 20132020000170)
  • National Institute of Biological Resources (NIBR)
  • Ministry of Environment (MOE) of the Republic of Korea (Award NIBR No. 2012-02-057)
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2015-05-01
2024-04-18
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Anaerosolibacter carboniphilus gen. nov., sp. nov., a strictly anaerobic iron-reducing bacterium isolated from coal-contaminated soil
Int J Syst Evol Microbiol 65, 1480 (2015); https://doi.org/10.1099/ijs.0.000124
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