1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_5

gen. nov., sp. nov., an acid-tolerant haloarchaeon isolated from commercial salt Free

Abstract

Three halophilic archaeal strains, MH2-243-1, MH2-93-1 and MH2-91-1 were isolated from commercial salt samples from Japan, Australia, and Bolivia. Strain MH2-243-1 was able to grow in the presence of 12–30 % (w/v) NaCl (optimum, 18 % NaCl), at pH 4.5–7.0 (optimum, pH 6.0) and at 20–60 °C (optimum, 40 °C). Strains MH2-91-1 and MH2-93-1 grew in slightly different ranges. The orthologous 16S rRNA gene sequences of the three strains were almost identical (99.8–99.9 % similarities), and the closest relative was JCM 16327 with 94.2–94.3 % 16S rRNA gene sequence similarities, followed by strains of members of the closely related genera and . The RNA polymerase subunit B gene () sequence also showed the highest similarity (86.6 %) to that of JCM 16327. The DNA G+C contents of strains MH2-243-1, MH2-93-1 and MH2-91-1 were 68.5, 68.8 and 68.3 mol%, respectively. DNA–DNA relatedness values amongst the three strains were 97–99 %. The polar lipids of the three strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, and at least seven unidentified glycolipids. The polar lipid composition differed from those of and species of the genera and . Based on the phenotypic and phylogenetic analyses, it is proposed that the isolates represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is MH2-243-1 ( = JCM 19596 = KCTC 4149) isolated from solar salt produced in Japan. MH2-93-1 ( = JCM 19595) and MH2-91-1 ( = JCM 19594) are additional strains of the type species.

  • Published Online:
Funding
This study was supported by the:
  • Grant-in-Aid for Young Scientists (Award B 25840142)
  • Japan Society for the Promotion of Science (JSPS)
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000152
2015-05-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/5/1640.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000152&mimeType=html&fmt=ahah

References

  1. Cline S. W., Schalkwyk L. C., Doolittle W. F. ( 1989 ). Transformation of the archaebacterium Halobacterium volcanii with genomic DNA. . J Bacteriol 171, 4987–4991.[PubMed]
    [Google Scholar]
  2. Dussault H. P. ( 1955 ). An improved technique for staining red halophilic bacteria. . J Bacteriol 70, 484–485.[PubMed]
    [Google Scholar]
  3. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  5. Gonzalez C., Gutierrez C., Ramirez C. ( 1978 ). Halobacterium vallismortis sp. nov. An amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. . Can J Microbiol 24, 710–715. [View Article] [PubMed]
    [Google Scholar]
  6. Grant W. D. ( 2001 ). Genus I. Halobacterium Elazari-Volcani 1957, 207AL emend. Larsen and Grant 1989, 2222. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 1, pp. 301–305. Edited by Boone D. R., Castenholz R. W., Garrity G. M. . New York:: Springer;.
     [Google Scholar]
  7. Gruber C., Legat A., Pfaffenhuemer M., Radax C., Weidler G. W., Busse H.-J., Stan-Lotter H. ( 2004 ). Halobacterium noricense sp. nov., an archaeal isolate from a bore core of an alpine Permian salt deposit, classification of Halobacterium sp. NRC-1 as a strain of H. salinarum and emended description of H. salinarum . . Extremophiles 8, 431–439. [View Article] [PubMed]
    [Google Scholar]
  8. Gutiérrez M. C., Ventosa A., Ruiz-Berraquero F. ( 1989 ). DNA-DNA homology studies among strains of Haloferax and other halobacteria. . Curr Microbiol 18, 253–256. [View Article]
    [Google Scholar]
  9. Kamekura M. ( 1993 ). Lipids of extreme halophiles. . In The Biology of Halophilic Bacteria, pp. 135–161. Edited by Vreeland R. H., Hochstein L. I. . Boca Raton, FL:: CRC Press;.
     [Google Scholar]
  10. Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A. et al. ( 2007 ). clustal w and clustal x version 2.0. . Bioinformatics 23, 2947–2948. [View Article] [PubMed]
    [Google Scholar]
  11. Minegishi H., Mizuki T., Echigo A., Fukushima T., Kamekura M., Usami R. ( 2008 ). Acidophilic haloarchaeal strains are isolated from various solar salts. . Saline Syst 4, 16. [View Article] [PubMed]
    [Google Scholar]
  12. Minegishi H., Echigo A., Nagaoka S., Kamekura M., Usami R. ( 2010 a). Halarchaeum acidiphilum gen. nov., sp. nov., a moderately acidophilic haloarchaeon isolated from commercial solar salt. . Int J Syst Evol Microbiol 60, 2513–2516. [View Article] [PubMed]
    [Google Scholar]
  13. Minegishi H., Kamekura M., Itoh T., Echigo A., Usami R., Hashimoto T. ( 2010 b). Further refinement of the phylogeny of the Halobacteriaceae based on the full-length RNA polymerase subunit B’ (rpoB’) gene. . Int J Syst Evol Microbiol 60, 2398–2408. [View Article] [PubMed]
    [Google Scholar]
  14. Minegishi H., Kamekura M., Kitajima-Ihara T., Nakasone K., Echigo A., Shimane Y., Usami R., Itoh T., Ihara K. ( 2012 ). Gene orders in the upstream of 16S rRNA genes divide genera of the family Halobacteriaceae into two groups. . Int J Syst Evol Microbiol 62, 188–195. [View Article] [PubMed]
    [Google Scholar]
  15. Minegishi H., Yamauchi Y., Echigo A., Shimane Y., Kamekura M., Itoh T., Ohkuma M., Usami R. ( 2013 ). Halarchaeum nitratireducens sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt. . Int J Syst Evol Microbiol 63, 4202–4206. [View Article] [PubMed]
    [Google Scholar]
  16. Miyazaki S., Sugawara H., Gojobori T., Tateno Y. ( 2003 ). DNA Data Bank of Japan (DDBJ) in XML. . Nucleic Acids Res 31, 13–16. [View Article] [PubMed]
    [Google Scholar]
  17. Oren A. ( 2012 ). Taxonomy of the family Halobacteriaceae: a paradigm for changing concepts in prokaryote systematics. . Int J Syst Evol Microbiol 62, 263–271. [View Article] [PubMed]
    [Google Scholar]
  18. Oren A., Ventosa A., Grant W. D. ( 1997 ). Proposed minimal standards for description of new taxa in the order Halobacteriales . . Int J Syst Bacteriol 47, 233–238. [View Article]
    [Google Scholar]
  19. Oren A., Arahal D. R., Ventosa A. ( 2009 ). Emended descriptions of genera of the family Halobacteriaceae. . Int J Syst Evol Microbiol 59, 637–642. [View Article] [PubMed]
    [Google Scholar]
  20. Pearson W. R., Lipman D. J. ( 1988 ). Improved tools for biological sequence comparison. . Proc Natl Acad Sci U S A 85, 2444–2448. [View Article] [PubMed]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  22. Saralov A. I., Baslerov R. V., Reutskikh E. M., Kuznetsov B. B. ( 2012 ). [ Halarchaeum solikamskense sp. nov., a thermotolerant neutrophilic haloarchaeon from the foamy products of flotation enrichment of potassium minerals. ]. Mikrobiologiia 81, 638–644 (in Russian).[PubMed]
    [Google Scholar]
  23. Shimane Y., Hatada Y., Minegishi H., Echigo A., Nagaoka S., Miyazaki M., Ohta Y., Maruyama T., Usami R. et al. ( 2011 ). Salarchaeum japonicum gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea isolated from commercial salt. . Int J Syst Evol Microbiol 61, 2266–2270. [View Article] [PubMed]
    [Google Scholar]
  24. Silvestro D., Michalak I. ( 2012 ). raxmlGUI: a graphical front-end for RAxML. . Org Divers Evol 12, 335–337. [View Article]
    [Google Scholar]
  25. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  26. Stamatakis A., Ludwig T., Meier H. ( 2005 ). RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees. . Bioinformatics 21, 456–463. [View Article] [PubMed]
    [Google Scholar]
  27. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125–128. [View Article]
    [Google Scholar]
  28. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  29. Yachai M., Tanasupawat S., Itoh T., Benjakul S., Visessanguan W., Valyasevi R. ( 2008 ). Halobacterium piscisalsi sp. nov., from fermented fish (pla-ra) in Thailand. . Int J Syst Evol Microbiol 58, 2136–2140. [View Article] [PubMed]
    [Google Scholar]
  30. Yamauchi Y., Minegishi H., Echigo A., Shimane Y., Shimoshige H., Kamekura M., Itoh T., Doukyu N., Inoue A., Usami R. ( 2013 a). Halarchaeum salinum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt. . Int J Syst Evol Microbiol 63, 1138–1142. [View Article] [PubMed]
    [Google Scholar]
  31. Yamauchi Y., Minegishi H., Echigo A., Shimane Y., Kamekura M., Itoh T., Ohkuma M., Doukyu N., Inoue A., Usami R. ( 2013 b). Halarchaeum rubridurum sp. nov., a moderately acidophilic haloarchaeon isolated from commercial sea salt samples. . Int J Syst Evol Microbiol 63, 3143–3147. [View Article] [PubMed]
    [Google Scholar]
  32. Yang Y., Cui H. L., Zhou P. J., Liu S. J. ( 2006 ). Halobacterium jilantaiense sp. nov., a halophilic archaeon isolated from a saline lake in Inner Mongolia, China. . Int J Syst Evol Microbiol 56, 2353–2355. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000152
Loading
Halocalculus aciditolerans gen. nov., sp. nov., an acid-tolerant haloarchaeon isolated from commercial salt
Int J Syst Evol Microbiol 65, 1640 (2015); https://doi.org/10.1099/ijs.0.000152
/content/journal/ijsem/10.1099/ijs.0.000152
/content/journal/ijsem/10.1099/ijs.0.000152
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error