RT Journal Article SR Electronic(1) A1 Modesto, Monica A1 Michelini, Samanta A1 Stefanini, Ilaria A1 Sandri, Camillo A1 Spiezio, Caterina A1 Pisi, Annamaria A1 Filippini, Gianfranco A1 Biavati, Bruno A1 Mattarelli, PaolaYR 2015 T1 Bifidobacterium lemurum sp. nov., from faeces of the ring-tailed lemur (Lemur catta) JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_6 SP 1726 OP 1734 DO https://doi.org/10.1099/ijs.0.000162 PB Microbiology Society, SN 1466-5034, AB Four Gram-positive-staining, microaerophilic, non-spore-forming, fructose-6-phosphate phosphoketolase-positive bacterial strains were isolated from a faecal sample of a 5-year-old ring-tailed lemur (Lemur catta). The strains showed a peculiar morphology, resembling a small coiled snake, a ring shape, or forming a little ‘Y’ shape. The isolated strains appeared identical, and LMC 13T was chosen as a representative strain and characterized further. Strain LMC 13T showed an A3β peptidoglycan type, similar to that found in Bifidobacterium longum . The DNA base composition was 57.2 mol% G+C. Almost-complete 16S rRNA, hsp60, rpoB, dnaJ, dnaG, purF, clpC and rpoC gene sequences were obtained, and phylogenetic relationships were determined. Comparative analysis of 16S rRNA gene sequences showed that strain LMC 13T showed the highest similarity to B. longum subsp. suis ATCC 27533T (96.65 %) and Bifidobacterium saguini DSM 23967T (96.64 %). Strain LMC 13T was located in an actinobacterial cluster and was more closely related to the genus Bifidobacterium than to other genera in the Bifidobacteriaceae . On the basis of these results, strain LMC 13T represents a novel species within the genus Bifidobacterium , for which the name Bifidobacterium lemurum sp. nov. is proposed; the type strain is LMC 13T ( = DSM 28807T = JCM 30168T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000162