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Volume 65, Issue Pt_6

sp. nov., an anaerobic, thermophilic, iron (III)-reducing bacterium isolated from deep-sea hydrothermal sulfide deposits Free

Abstract

A thermophilic, anaerobic, iron-reducing bacterium (strain DY22619) was isolated from a sulfide sample collected from an East Pacific Ocean hydrothermal field at a depth of 2901 m. Cells were Gram-stain-negative, motile rods (2–10 µm in length, 0.5 µm in width) with multiple peritrichous flagella. The strain grew at 40–70 °C inclusive (optimum 60 °C), at pH 4.5–8.5 inclusive (optimum pH 7.0) and with sea salts concentrations of 1–10 % (w/v) (optimum 3 % sea salts) and NaCl concentrations of 1.5–5.0 % (w/v) (optimum 2.5 % NaCl). Under optimal growth conditions, the generation time was around 55 min. The isolate was an obligate chemoorganoheterotroph, utilizing complex organic compounds, amino acids, carbohydrates and organic acids including peptone, tryptone, beef extract, yeast extract, alanine, glutamate, methionine, threonine, fructose, mannose, galactose, glucose, palatinose, rhamnose, turanose, gentiobiose, xylose, sorbose, pyruvate, tartaric acid, α-ketobutyric acid, α-ketovaleric acid, galacturonic acid and glucosaminic acid. Strain DY22619 was strictly anaerobic and facultatively dependent on various forms of Fe(III) as an electron acceptor: insoluble forms and soluble forms. It did not reduce sulfite, sulfate, thiosulfate or nitrate. The genomic DNA G+C content was 29.0 mol%. Phylogenetic 16S rRNA gene sequence analyses revealed that the closest relative of strain DY22619 was MV1087, sharing 97.41 % 16S rRNA gene sequence similarity. On the basis of physiological distinctness and phylogenetic distance, the isolate is considered to represent a novel species of the genus , for which the name http://dx.doi.org/10.1601/nm.4081 sp. nov. is proposed. The type strain is DY22619 ( = JCM 19467 = DSM 27799 = MCCC1A06455).

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Funding
This study was supported by the:
  • National Program on Key Basic Research Project (Award 2012CB417304)
  • National High Technology Research and Development Program of China (Award 2012AA092102)
  • National Infrastructure of Microbial Resources (Award NIRM2014-9)
  • EU FP7 program MaCuMBA (Award 311975)
  • PICS-CNRS-INEE Phypress
  • PHC Cai YuanPei 30412WG
© 2015 IUMS
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2015-06-01
2024-04-19
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Caloranaerobacter ferrireducens sp. nov., an anaerobic, thermophilic, iron (III)-reducing bacterium isolated from deep-sea hydrothermal sulfide deposits
Int J Syst Evol Microbiol 65, 1714 (2015); https://doi.org/10.1099/ijs.0.000165
/content/journal/ijsem/10.1099/ijs.0.000165
/content/journal/ijsem/10.1099/ijs.0.000165
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