%0 Journal Article %A Suriyachadkun, Chanwit %A Ngaemthao, Wipaporn %A Chunhametha, Suwanee %A Thawai, Chitti %A Sanglier, Jean-Jacques %T Sinosporangium fuscum sp. nov., isolated from soil %D 2015 %J International Journal of Systematic and Evolutionary Microbiology, %V 65 %N Pt_6 %P 1764-1769 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.000172 %I Microbiology Society, %X A novel actinomycete, A-T 8343T was isolated from a moist evergreen forest soil sample collected in the Trat Province, Thailand. Based on 16S rRNA gene sequence analysis, strain A-T 8343T belonged to the genus Sinosporangium and was closely related to Sinosporangium siamense A-T 1946T (98.81 %) and Sinosporangium album 6014T (98.54 %). The DNA–DNA relatedness values were 21.8–27 % with S. siamense A-T 1946T and 31.1–31.9 % with S. album 6014T, which were significantly below 70 %. The result differentiated A-T 8343T from the closest species. The organism developed spherical sporangia containing non-motile spores on aerial mycelia. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, madurose and glucose. The predominant menaquinones were MK-9(H2) and MK-9(H4). The diagnostic phospholipids were phosphatidylmethylethanolamine, phosphatidylethanolamine, hydroxyl-phosphatidylethanolamine, diphosphatidylglycerol, lyso-phosphatidylethanolamine, N-acetylglucosamine-containing phospholipids, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol-mannosides, aminophosphoglycolipid and one unknown phospholipid. The major cellular fatty acids were saturated C16 : 0, iso C16 : 0, unsaturated C16 : 1 and C18 : 1. Following an evaluation of phenotypic, chemotaxonomic and genotypic characteristics, the new isolate is proposed as a representative novel species of the genus Sinosporangium to be named Sinosporangium fuscum sp. nov. The type strain is A-T 8343T ( = BCC 52770T = NBRC 109516T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000172