1887

Abstract

A Gram-stain-negative, aerobic, yellow-pigmented, rod-shaped bacterium, designated strain SC_3, was isolated from pesticide-contaminated soil sediment. The strain was able to mineralize 2-phenoxybenzoic acid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SC_3 formed a monophyletic lineage in the genus , and showed highest similarity to the type strains of (97.0 %), followed by (96.8 %) and (96.7 %). The DNA–DNA relatedness between strain SC_3 and its closest phylogenetic neighbours was lower than 70 %. The major fatty acids (>5 % of the total) were summed feature 8 (comprising Cω7/Cω6), summed feature 3 (comprising Cω7/Cω6), C 2-OH, C and Cω6. The predominant quinone was ubiquinone Q-10, and the major polyamine was spermidine. The polar lipid profile contained diphosphatidylglycerol (DPG), sphingoglycolipid (SGL), phosphatidylethanolamine (PDME), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PMME), an unknown aminolipid (AL), two unknown lipids (L1, L2) and several unknown phospholipids (PL1–6). The genomic DNA G+C content of strain SC_3 was 62.9 mol%. On the basis of phenotypic, chemotaxonomic, phylogenetic and genotypic data, strain SC_3 represents a novel species of the genus , for which the name Sphingobium phenoxybenzoativorans sp. nov. is proposed. The type strain is SC_3 ( = CCTCC AB 2014349 = KACC 42448).

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2015-06-01
2024-03-29
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