@article{mbs:/content/journal/ijsem/10.1099/ijs.0.000231, author = "Surussawadee, Janjira and Jindamorakot, Sasitorn and Nakase, Takashi and Lee, Ching-Fu and Limtong, Savitree", title = "Hannaella phyllophila sp. nov., a basidiomycetous yeast species associated with plants in Thailand and Taiwan", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_7", pages = "2135-2140", doi = "https://doi.org/10.1099/ijs.0.000231", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000231", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Five strains representing one novel anamorphic yeast species were isolated from plant leaves collected in Thailand (strains DMKU-SP186T, ST-111 and ST-201) and Taiwan (strains FN20L02 and SM13L16). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were assigned to a single novel species of the genus Hannaella. The sequences of the D1/D2 regions of the LSU rRNA genes of four of the strains (DMKU-SP186T, ST-111, FN20L02 and SM13L16) were identical, while differing from strain ST-201 by 2 substitutions and 2 gaps. The nucleotide sequence of the ITS regions of the five strains differed from each other by between 0 and 3 nucleotide substitutions. The novel species was most closely related to Hannaella luteola, but showed 1.0–1.3 % nucleotide substitutions (between 6 substitutions out of 568–606 nt and 8 substitutions, and 2 gaps out of 597 nt) in the D1/D2 region of the LSU rRNA gene and 1.4–2.0 % nucleotide substitutions (6–9 substitutions out of 435 nt) in the ITS region. Ballistospores were produced by three of the strains on cornmeal agar at 15 and 20 °C after 4 weeks, while H. luteola did not produce ballistospores. The name Hannaella phyllophila sp. nov. is proposed. The type strain is DMKU-SP186T ( = BCC 69500T = NBRC 110428T = CBS 13921T).", }