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Volume 65, Issue Pt_7

gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from coastal sediment Free

Abstract

A Gram-stain-negative, facultatively anaerobic, non-motile and pink-pigmented bacterium, designated strain HF08, was isolated from marine sediment of the coast of Weihai, China. Cells were rod-shaped, and oxidase- and catalase-positive. The isolate grew optimally at 33 °C, at pH 7.5–8.0 and with 2–3 % (w/v) NaCl. The dominant cellular fatty acids were iso-C, anteiso-C and iso-C. Menaquinone 7 (MK-7) was the major respiratory quinone and the DNA G+C content was 44.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of the class , and shared 88–90 % sequence similarity with the closest genera , , , and Based on the phylogenetic and phenotypic evidence presented, a novel species in a new genus of the family is proposed, with the name gen. nov., sp. nov. The type strain of is HF08 ( = KCTC 42261 = CICC 10901).

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2015-07-01
2024-04-18
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References

  1. Chang D.-H., Lee J.-B., Lee G.-H., Rhee M.-S., Lee H., Bae K.-S., Park D.-S., Kim B.-C. ( 2013;). Sunxiuqinia dokdonensis sp. nov., isolated from deep sub-seafloor sediment. J Microbiol 51 741–746 [View Article] [PubMed].
     [Google Scholar]
  2. da Costa M.S., Nobre M.F., Wait R. ( 2006;). Analysis of lipids from extremophilic bacteria. Methods Microbiol 35 127–159 [View Article].
     [Google Scholar]
  3. Dong X.-Z., Cai M.-Y. ( 2001;). Determination of biochemical characteristics. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Edited by Dong X. -Z., Cai M. -Y. Beijing: Science Press (in Chinese);.
     [Google Scholar]
  4. Du Z.-J., Wang Y., Dunlap C., Rooney A.P., Chen G.-J. ( 2014;). Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64 1690–1696 [View Article] [PubMed].
     [Google Scholar]
  5. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed].
     [Google Scholar]
  6. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  7. Hall T.A. ( 1999;). BioEdit: a user-friendly sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41 95–98.
     [Google Scholar]
  8. Holmes D.E., Nevin K.P., Woodard T.L., Peacock A.D., Lovley D.R. ( 2007;). Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell. Int J Syst Evol Microbiol 57 701–707 [View Article] [PubMed].
     [Google Scholar]
  9. Huang X.-F., Liu Y.-J., Dong J.-D., Qu L.-Y., Zhang Y.-Y., Wang F.-Z., Tian X.-P., Zhang S. ( 2014;). Mangrovibacterium diazotrophicum gen. nov., sp. nov., a nitrogen-fixing bacteriu isolated from a mangrove sediment, and proposal of Prolixibacteraceae fam. nov. Int J Syst Evol Microbiol 64 875–881 [View Article] [PubMed].
     [Google Scholar]
  10. Iino T., Mori K., Itoh T., Kudo T., Suzuki K., Ohkuma M. ( 2014;). Description of Mariniphaga anaerophila gen. nov., sp. nov., a facultatively aerobic marine bacterium isolated from tidal flat sediment, reclassification of the Draconibacteriaceae as a later heterotypic synonym of the Prolixibacteraceae and description of the family Marinifilaceae fam. nov. Int J Syst Evol Microbiol 64 3660–3667 [View Article] [PubMed].
     [Google Scholar]
  11. Irgens R.L. ( 1977;). Meniscus, a new genus of aerotolerant, gas-vacuolated bacteria. Int J Syst Bacteriol 27 38–43 [View Article].
     [Google Scholar]
  12. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y.S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  13. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120 [View Article] [PubMed].
     [Google Scholar]
  14. Kroppenstedt R.M. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5 2359–2367 [View Article].
     [Google Scholar]
  15. Liu Q.-Q., Wang Y., Li J., Du Z.-J., Chen G.-J. ( 2014a;). Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis Saccharicrinis fermentans . Int J Syst Evol Microbiol 64 2204–2209 [View Article] [PubMed].
     [Google Scholar]
  16. Liu Q.-Q., Li X.L., Rooney A.P., Du Z.-J., Chen G.-J. ( 2014b;). Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae . Int J Syst Evol Microbiol 64 3473–3477 [View Article] [PubMed].
     [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159–167 [View Article].
     [Google Scholar]
  18. Minnikin D., O'Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  19. Na H., Kim S., Moon E.Y., Chun J. ( 2009;). Marinifilum fragile gen. nov., sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 59 2241–2246 [View Article] [PubMed].
     [Google Scholar]
  20. Qu L., Zhu F., Hong X., Gao W., Chen J., Sun X. ( 2011;). Sunxiuqinia elliptica gen. nov., sp. nov., a member of the phylum Bacteroidetes isolated from sediment in a sea cucumber farm. Int J Syst Evol Microbiol 61 2885–2889 [View Article] [PubMed].
     [Google Scholar]
  21. Ruvira M.A., Lucena T., Pujalte M.J., Arahal D.R., Macián M.C. ( 2013;). Marinifilum flexuosum sp. nov., a new Bacteroidetes isolated from coastal Mediterranean Sea water and emended description of the genus Marinifilum Na et al., 2009. Syst Appl Microbiol 36 155–159 [View Article] [PubMed].
     [Google Scholar]
  22. Smibert R.M., Krieg N.R. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
     [Google Scholar]
  23. Takai K., Abe M., Miyazaki M., Koide O., Nunoura T., Imachi H., Inagaki F., Kobayashi T. ( 2013;). Sunxiuqinia faeciviva sp. nov., a facultatively anaerobic organoheterotroph of the Bacteroidetes isolated from deep subseafloor sediment. Int J Syst Evol Microbiol 63 1602–1609 [View Article] [PubMed].
     [Google Scholar]
  24. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 2725–2729 [View Article] [PubMed].
     [Google Scholar]
  25. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed].
     [Google Scholar]
  26. Wang Z.-J., Xie Z.-H., Wang C., Du Z.-J., Chen G.-J. ( 2014;). Motiliproteus sediminis gen. nov., sp. nov., isolated from coastal sediment. Antonie van Leeuwenhoek 106 615–621 [View Article] [PubMed].
     [Google Scholar]
  27. Yoon J., Kasai H. ( 2014;). Sunxiuqinia rutila sp. nov., a new member of the phylum Bacteroidetes isolated from marine sediment. J Gen Appl Microbiol 60 28–32 [View Article] [PubMed].
     [Google Scholar]
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Roseimarinus sediminis gen. nov., sp. nov., a facultatively anaerobic bacterium isolated from coastal sediment
Int J Syst Evol Microbiol 65, 2260 (2015); https://doi.org/10.1099/ijs.0.000250
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