1887

Abstract

A novel marine actinomycete, designated B374, was isolated from a marine sponge, , which was collected from São Paulo, Brasil. The taxonomic position of B374 was established by using data derived from a polyphasic approach. The organism showed a combination of chemotaxonomic and morphological characteristics consistent with its classification in the genus and it formed a distinct phyletic line in the clade of the genus , based on 16S rRNA gene sequences. Strain B374 was most closely related to SST-45 (98.5 % 16S rRNA gene sequence similarity), but was distinguished from this strain and from the other type strains of species of the genus on the basis of a combination of phenotypic properties. The data obtained, therefore, indicates that isolate B374 ( = CBMAI 1089 = DSM 28169) should be classified as a novel species of the genus , for which the name sp. nov. is proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000254
2015-07-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/7/2286.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000254&mimeType=html&fmt=ahah

References

  1. Dastager S.G., Lee J.-C., Ju Y.-J., Park D.-J., Kim C.-J. ( 2008;). Marmoricola bigeumensis sp. nov., a member of the family Nocardioidaceae . Int J Syst Evol Microbiol 58 10601063. [CrossRef]
    [Google Scholar]
  2. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376. [CrossRef]
    [Google Scholar]
  3. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evol 39 783791. [CrossRef]
    [Google Scholar]
  4. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for specific tree topology. Syst Zool 20 406416. [CrossRef]
    [Google Scholar]
  5. Gonzalez J.M., Saiz-Jimenez C. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4 770773. [CrossRef]
    [Google Scholar]
  6. Gordon R.E., Mihm J.M. ( 1962;). The type species of the genus Nocardia . J Gen Microbiol 27 110. [CrossRef]
    [Google Scholar]
  7. Jukes T.H., Cantor C.R. ( 1969;). Evolution of protein molecules. . In Mammalian Protein Metabolism, pp. 21132. Edited by Munro H. N. vol. 3 New York: Academic Press;. [CrossRef]
    [Google Scholar]
  8. Kim B.Y., Stach J.E.M., Weon H.Y., Kwon S.W., Goodfellow M. ( 2010;). Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil. Int J Syst Evol Microbiol 60 18131823. [CrossRef]
    [Google Scholar]
  9. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721. [CrossRef]
    [Google Scholar]
  10. Lane D.J. ( 1991;). 16S/23S sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;.
    [Google Scholar]
  11. Lee S.D. ( 2007;). Marmoricola aequoreus sp. nov., a novel actinobacterium isolated from marine sediment. Int J Syst Evol Microbiol 57 13911395. [CrossRef]
    [Google Scholar]
  12. Lee D.W., Lee S.D. ( 2010;). Marmoricola scoriae sp. nov., isolated from volcanic ash. Int J Syst Evol Microbiol 60 21352139. [CrossRef]
    [Google Scholar]
  13. Lee S.D., Lee D.W., Ko Y.-H. ( 2011;). Marmoricola korecus sp. nov. Int J Syst Evol Microbiol 61 16281631. [CrossRef]
    [Google Scholar]
  14. Meier-Kolthoff J.P., Göker M., Spröer C., Klenk H.-P. ( 2013;). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195 413418. [CrossRef]
    [Google Scholar]
  15. Menezes C.B.A., Bonugli-Santos R.C., Miqueletto P.B., Passarini M.R.Z., Silva C.H., Justo M.R., Leal R.R., Fantinatti-Garboggini F., Oliveira V.M., other authors. ( 2010;). Microbial diversity associated with algae, ascidians and sponges from the north coast of São Paulo state, Brazil. Microbiol Res 165 466482. [CrossRef]
    [Google Scholar]
  16. Minnikin D.E., Hutchinson I.G., Caldicott A.B., Goodfellow M. ( 1980;). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr A 188 221233. [CrossRef]
    [Google Scholar]
  17. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241. [CrossRef]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425.
    [Google Scholar]
  19. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  20. Shirling E.B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313340. [CrossRef]
    [Google Scholar]
  21. Staneck J.L., Roberts G.D. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28 226231.
    [Google Scholar]
  22. Tamura K., Nei M. ( 1993;). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10 512526.
    [Google Scholar]
  23. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729. [CrossRef]
    [Google Scholar]
  24. Uchida K., Kudo T., Suzuki K.I., Nakase T. ( 1999;). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. J Gen Appl Microbiol 45 4956. [CrossRef]
    [Google Scholar]
  25. Urzì C., Salamone P., Schumann P., Stackebrandt E. ( 2000;). Marmoricola aurantiacus gen. nov., sp. nov., a coccoid member of the family Nocardioidaceae isolated from a marble statue. Int J Syst Evol Microbiol 50 529536. [CrossRef]
    [Google Scholar]
  26. van Soolingen D., de Haas P.E.W., Hermans P.W.M., Groenen P.M., van Embden J.D.A. ( 1993;). Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J Clin Microbiol 31 19871995.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000254
Loading
/content/journal/ijsem/10.1099/ijs.0.000254
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error