1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_8

sp. nov., isolated from soil Free

Abstract

Two bacterial strains (JC247 and JC248) were isolated from soil samples collected from Rann of Kutch, Gujarat, India. Colonies of both strains were creamy white. Cells were Gram-stain-positive, rods-to-curved rods (crescent-shaped), and produced centrally located oval-shaped endospores. Major (>5 %) fatty acids of both strains were iso-C    , iso-C    , iso-C    , C    ω11 and C    , with minor ( < 5 but >1 %) amounts of anteiso-C    , anteiso-C    , iso-C    H, iso-C    , iso-C    , C    , C    , C    , C    ω9, iso-C    ω10 and anteiso-C    B/isoI. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids of both strains. Cell-wall amino acids were -alanine, -alanine, -glutamic acid and -diaminopimelic acid. The genomic DNA G+C content of strains JC247 and JC248 was 48.2 and 48.1 mol%, respectively. Both strains were closely related with mean DNA–DNA hybridization >90 %. 16S rRNA gene sequence analysis of both strains indicated that they are members of the genus within the family of the phylum . Both strains had a 16S rRNA gene sequence similarity of 96.93 % with NCIMB 9366 and < 96.92 % with other members of the genus . Sequence similarity between strain JC247 and JC248 was 100 %. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strains JC247 and JC248 as representatives of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC247 ( = KCTC 33627 = LMG 28608).

  • Published Online:
© 2015 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000296
2015-08-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2531.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000296&mimeType=html&fmt=ahah

References

  1. Amoozegar M.A., Didari M., Bagheri M., Fazeli S.A.S.F., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A. ( 2013;). Bacillus salsus sp. nov., a halophilic bacterium from a hypersaline lake. Int J Syst Evol Microbiol 63 3324–3329 [View Article] [PubMed].
     [Google Scholar]
  2. Cappuccino J.G., Sherman N. ( 1998). Microbiology: a laboratory manual , 5th edition. Menlo Park, Ca: Benjamin/Cummings;.
     [Google Scholar]
  3. Carrasco I.J., Márquez M.C., Xue Y., Ma Y., Cowan D.A., Jones B.E., Grant W.D., Ventosa A. ( 2007;). Bacillus chagannorensis sp. nov., a moderate halophile from a soda lake in Inner Mongolia, China. Int J Syst Evol Microbiol 57 2084–2088 [View Article] [PubMed].
     [Google Scholar]
  4. Chakravarthy S.K., Ramaprasad E.V.V., Shobha E., Sasikala Ch., Ramana ChV. ( 2012;). Rhodoplanes piscinae sp. nov. isolated from pond water. Int J Syst Evol Microbiol 62 2828–2834 [View Article] [PubMed].
     [Google Scholar]
  5. Dastager S.G., Mawlankar R., Tang S.K., Srinivasan K., Ramana V.V., Shouche Y.S. ( 2014;). Bacillus enclensis sp. nov., isolated from sediment sample. Antonie van Leeuwenhoek 105 199–206 [View Article] [PubMed].
     [Google Scholar]
  6. Edgar R.C. ( 2004;). muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32 1792–1797 [View Article] [PubMed].
     [Google Scholar]
  7. Hugh R., Leifson E. ( 1953;). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram negative bacteria. J Bacteriol 66 24–26 [PubMed].
     [Google Scholar]
  8. Kämpfer P., Rosselló-Mora R., Falsen E., Busse H.-J., Tindall B.J. ( 2006;). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov.. Int J Syst Evol Microbiol 56 781–786 [View Article] [PubMed].
     [Google Scholar]
  9. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  10. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111–120 [View Article] [PubMed].
     [Google Scholar]
  11. Kosowski K., Schmidt M., Pukall R., Hause G., Kämpfer P., Lechner U. ( 2014;). Bacillus pervagus sp. nov. and Bacillus andreesenii sp. nov., isolated from a composting reactor. Int J Syst Evol Microbiol 64 88–94 [View Article] [PubMed].
     [Google Scholar]
  12. Lane D.J., Pace B., Olsen G.J., Stahl D.A., Sogin M.L., Pace N.R. ( 1985;). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82 6955–6959 [View Article] [PubMed].
     [Google Scholar]
  13. Logan N.A., Berge O., Bishop A.H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59 2114–2121 [View Article] [PubMed].
     [Google Scholar]
  14. Madhaiyan M., Poonguzhali S., Lee J.-S., Lee K.-C., Hari K. ( 2011;). Bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil. Antonie van Leeuwenhoek 100 437–444 [View Article] [PubMed].
     [Google Scholar]
  15. Manickam N., Singh N.K., Bajaj A., Kumar R.M., Kaur G., Kaur N., Bala M., Kumar A., Mayilraj S. ( 2014;). Bacillus mesophilum sp. nov., strain IITR-54T, a novel 4-chlorobiphenyl dechlorinating bacterium. Arch Microbiol 196 517–523 [View Article] [PubMed].
     [Google Scholar]
  16. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208–218 [View Article].
     [Google Scholar]
  17. McKerrow J., Vagg S., McKinney T., Seviour E.M., Maszenan A.M., Brooks P., Seviour R.J. ( 2000;). A simple HPLC method for analysing diaminopimelic acid diastereomers in cell walls of Gram-positive bacteria. Lett Appl Microbiol 30 178–182 [View Article] [PubMed].
     [Google Scholar]
  18. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159–167 [View Article].
     [Google Scholar]
  19. Priest F.G., Goodfellow M., Todd C. ( 1988;). A numerical classification of the genus Bacillus . J Gen Microbiol 134 1847–1882 [PubMed].
     [Google Scholar]
  20. Reddy S.V., Thirumala M., Farooq M., Sasikala C., Ramana C.V. ( 2015;). Bacillus lonarensis sp. nov., an alkalitolerant bacterium isolated from a soda lake. Arch Microbiol 197 27–34 [View Article] [PubMed].
     [Google Scholar]
  21. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  22. Schleifer K.H. ( 1985;). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18 123–156 [View Article].
     [Google Scholar]
  23. Schleifer K.H., Kandler O. ( 1972;). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36 407–477 [PubMed].
     [Google Scholar]
  24. Seldin L., Dubnau D. ( 1985;). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35 151–154 [View Article].
     [Google Scholar]
  25. Sonalkar V.V., Mawlankar R., Venkata Ramana V., Joseph N., Shouche Y.S., Dastager S.G. ( 2015;). Bacillus filamentosus sp. nov., isolated from sediment sample. Antonie van Leeuwenhoek 107 433–441 [View Article] [PubMed].
     [Google Scholar]
  26. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch.V. ( 2013;). Falsirhodobacter halotolerans gen. nov., sp. nov., isolated from dry soils of a solar saltern. Int J Syst Evol Microbiol 63 2132–2137 [View Article] [PubMed].
     [Google Scholar]
  27. Subhash Y., Sasikala Ch., Ramana Ch.V. ( 2014;). Bacillus luteus sp. nov., isolated from soil. Int J Syst Evol Microbiol 64 1580–1586 [View Article] [PubMed].
     [Google Scholar]
  28. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 2725–2729 [View Article] [PubMed].
     [Google Scholar]
  29. Täubel M., Kämpfer P., Buczolits S., Lubitz W., Busse H.-J. ( 2003;). Bacillus barbaricus sp. nov., isolated from an experimental wall painting. Int J Syst Evol Microbiol 53 725–730 [View Article] [PubMed].
     [Google Scholar]
  30. Zhai L., Liao T., Xue Y., Ma Y. ( 2012;). Bacillus daliensis sp. nov., an alkaliphilic, Gram-positive bacterium isolated from a soda lake. Int J Syst Evol Microbiol 62 949–953 [View Article] [PubMed].
     [Google Scholar]
  31. Zhang J., Wang J., Fang C., Song F., Xin Y., Qu L., Ding K. ( 2010;). Bacillus oceanisediminis sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 60 2924–2929 [View Article] [PubMed].
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000296
Loading
Bacillus crescens sp. nov., isolated from soil
Int J Syst Evol Microbiol 65, 2531 (2015); https://doi.org/10.1099/ijs.0.000296
/content/journal/ijsem/10.1099/ijs.0.000296
/content/journal/ijsem/10.1099/ijs.0.000296
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error