1887

Abstract

Strain M-SA3-94, an aerobic, Gram-stain-positive, ovoid- to rod-shaped, non-motile bacterium, was isolated from the marine sediment of Ardley cove, King George Island, Antarctica. Strain M-SA3-94 grew optimally at pH 5.0–6.0, 20 °C and in the presence of 1.0 % (w/v) NaCl. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that strain M-SA3-94 belonged to the genus in the family , clustering with NCIMB 12834, BX5-10, CL-GY44 and D94-1 (with 96.1 %, 95.9 %, 94.5 % and 94.7 % 16S rRNA gene sequence similarity, respectively). The chemotaxonomic properties of strain M-SA3-94 were similar to those of members of the genus with validly published names. The major fatty acid was iso-C. The polar lipid pattern contained diphosphatidylglycerol, phosphatidylglycerol and three unknown phospholipids. The diagnostic diamino acid in the cell-wall peptidoglycan was -2, 6-diaminopimelic acid. MK-8(H4) was the predominant menaquinone and the DNA G+C content of this strain was 66.7 mol%. On the basis of these phenotypic, phylogenetic and chemotaxonomic data, strain M-SA3-94 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M-SA3-94 ( = CCTCC AB2014053 = LMG 28254).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000309
2015-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/8/2615.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000309&mimeType=html&fmt=ahah

References

  1. Bauer A.W., Kirby W.M.M., Sherris J.C., Turck M. ( 1966;). Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45 493496 [PubMed].
    [Google Scholar]
  2. Bowman J.P. ( 2000;). ). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50 18611868 [PubMed]. [CrossRef]
    [Google Scholar]
  3. Busse H.-J., Schumann P. ( 1999;). Polyamine profiles within genera of the class Actinobacteria with -diaminopimelic acid in the peptidoglycan. Int J Syst Bacteriol 49 179184 [View Article] [PubMed].
    [Google Scholar]
  4. Cappuccino J.G., Sherman N. ( 2002). Microbiology: a Laboratory Manual , 6th edn. Menlo Park, CA: Benjamin/Cummings;.
    [Google Scholar]
  5. Cho Y., Jang G.I., Cho B.C. ( 2013;). Nocardioides marinquilinus sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 63 25942599 [View Article] [PubMed].
    [Google Scholar]
  6. Chou J.H., Cho N.T., Arun A.B., Young C.C., Chen W.M. ( 2008;). Nocardioides fonticola sp. nov., a novel actinomycete isolated from spring water. Int J Syst Evol Microbiol 58 18641868 [View Article] [PubMed].
    [Google Scholar]
  7. Collins M.D., Keddie R.M., Kroppenstedt R.M. ( 1983;). Lipid composition of Arthrobacter simplex, Arthrobacter tumescens and possibly related taxa. Syst Appl Microbiol 4 1826 [View Article] [PubMed].
    [Google Scholar]
  8. Collins M.D., Cockcroft S., Wallbanks S. ( 1994;). Phylogenetic analysis of a new LL-diaminopimelic acid-containing coryneform bacterium from herbage, Nocardioides plantarum sp. nov. Int J Syst Bacteriol 44 523526 [View Article] [PubMed].
    [Google Scholar]
  9. Dastager S.G., Lee J.C., Ju Y.J., Park D.J., Kim C.J. ( 2008;). Nocardioides koreensis sp. nov., Nocardioides bigeumensis sp. nov. and Nocardioides agariphilus sp. nov., isolated from soil from Bigeum Island, Korea. Int J Syst Evol Microbiol 58 22922296 [View Article] [PubMed].
    [Google Scholar]
  10. Dastager S.G., Lee J.C., Ju Y.J., Park D.J., Kim C.J. ( 2009;). Nocardioides sediminis sp. nov., isolated from a sediment sample. Int J Syst Evol Microbiol 59 280284 [View Article] [PubMed].
    [Google Scholar]
  11. Doetsch R.N. ( 1981;). Determinative methods of light microscopy. . In Manual of Methods for General Bacteriology, pp. 2133. Edited by Gerhardt P, Murray R. G. E, Costilow R. N, Nester E. W, Wood W. A, Krieg N. R, Phillips G. H. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  12. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed].
    [Google Scholar]
  13. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  14. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T., Williams S.T. ( 1994;). Nocardioform actinomycetes. . In Shorter Bergey's Manual of Determinative Bacteriology, pp. 625650. Edited by Hensyl W. R. Baltimore: Williams & Wilkins;.
    [Google Scholar]
  15. Hopwood D.A., Bibb M.J., Chater K.F., Kieser T., Bruton C.J., Kieser H.M., Lydiate D.J., Smith C.P., Ward J.M., Schrempf H., (editors). ( 1985). Genetic Manipulation of Streptomyces. A Laboratory Manual Norwich: John Innes Foundation;.
    [Google Scholar]
  16. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  17. Komagata K., Suzuki K. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161207 [View Article].
    [Google Scholar]
  18. Mesbah M., Whitman W.B. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. J Chromatogr A 479 297306 [View Article] [PubMed].
    [Google Scholar]
  19. Minnikin D., O'Donnell A., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  20. Prauser H. ( 1976;). Nocardioides, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26 5865 [View Article].
    [Google Scholar]
  21. Rzhetsky A., Nei M. ( 1992;). A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9 945967.
    [Google Scholar]
  22. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  23. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  24. Schippers A., Schumann P., Spröer C. ( 2005;). Nocardioides oleivorans sp. nov., a novel crude-oil-degrading bacterium. Int J Syst Evol Microbiol 55 15011504 [View Article] [PubMed].
    [Google Scholar]
  25. Song G.C., Yasir M., Bibi F., Chung E.J., Jeon C.O., Chung Y.R. ( 2011;). Nocardioides caricicola sp. nov., an endophytic bacterium isolated from a halophyte, Carex scabrifolia Steud. Int J Syst Evol Microbiol 61 105109 [View Article] [PubMed].
    [Google Scholar]
  26. Stackebrandt E., Rainey F.A., Ward-Rainey N.L. ( 1997;). Proposal for a new hierarchic classification system, Actinobacteria classic nov. Int J Syst Bacteriol 47 479491 [View Article].
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  28. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed].
    [Google Scholar]
  29. Tindall B.J. ( 1990;). Lipid composition of Halobacterium lacusprofundi . FEMS Microbiol Lett 66 199202 [View Article].
    [Google Scholar]
  30. Tóth E.M., Kéki Z., Homonnay Z.G., Borsodi A.K., Márialigeti K., Schumann P. ( 2008;). Nocardioides daphniae sp. nov., isolated from Daphnia cucullata (Crustacea: Cladocera). Int J Syst Evol Microbiol 58 7883 [View Article] [PubMed].
    [Google Scholar]
  31. Xie C.H., Yokota A. ( 2003;). Phylogenetic analyses of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49 345349 [View Article] [PubMed].
    [Google Scholar]
  32. Yoon J.H., Cho Y.G., Lee S.T., Suzuki K., Nakase T., Park Y.H. ( 1999;). Nocardioides nitrophenolicus sp. nov., a p-nitrophenol-degrading bacterium. Int J Syst Bacteriol 49 675680 [View Article] [PubMed].
    [Google Scholar]
  33. Yoon J.H., Kim I.G., Lee M.H., Lee C.H., Oh T.K. ( 2005;). Nocardioides alkalitolerans sp nov., isolated from an alkaline serpentinite soil in Korea. Int J Syst Evol Micr 55 809814. [CrossRef]
    [Google Scholar]
  34. Zhang D.C., Schumann P., Redzic M., Zhou Y.G., Liu H.C., Schinner F., Margesin R. ( 2012a;). Nocardioides alpinus sp. nov., a psychrophilic actinomycete isolated from alpine glacier cryoconite. Int J Syst Evol Microbiol 62 445450 [View Article] [PubMed].
    [Google Scholar]
  35. Zhang J., Ma Y., Yu H. ( 2012b;). Nocardioides lianchengensis sp. nov., an actinomycete isolated from soil. Int J Syst Evol Microbiol 62 26982702 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000309
Loading
/content/journal/ijsem/10.1099/ijs.0.000309
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error