RT Journal Article SR Electronic(1) A1 Zhong, Zhi-Ping A1 Liu, Ying A1 Liu, Hong-Can A1 Wang, Fang A1 Zhou, Yu-Guang A1 Liu, Zhi-PeiYR 2015 T1 Vibrio salilacus sp. nov., a new member of the Anguillarum clade with six alleles of the 16S rRNA gene from a saline lake JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_8 SP 2653 OP 2660 DO https://doi.org/10.1099/ijs.0.000316 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, catalase- and oxidase-positive, facultatively aerobic bacterium, strain DSG-S6T, was isolated from Dasugan Lake (salinity 3.1 %, w/w), China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain DSG-S6T were non-spore-forming, slightly bent rods, and motile by means of a single polar flagellum. Growth occurred in the presence of 0–7.0  % (w/v) NaCl (optimum, 2.0  %), at 4–35 °C (optimum, 30 °C) and at pH 6.0–10.5 (optimum, pH 8.0–8.5). C16 : 0, C18 : 1ω7c and C16 : 1ω7c and/or C16 : 1ω6c were the major fatty acids. Six alleles of the 16S rRNA gene sharing 98.9–99.9  % similarity were detected in strain DSG-S6T, which showed highest 16S rRNA gene sequence similarity to Vibrio aestuarianus ATCC 35048T (97.7  %), then to Vibrio pacinii LMG 19999T (97.6  %) and Vibrio metschnikovii CIP 69.14T (96.8  %). Multilocus sequence analysis of four housekeeping genes and 16S rRNA genes clearly clustered it as a member of the Anguillarum clade. Mean DNA–DNA relatedness between strain DSG-S6T and V. aestuarianus NBRC 15629T, V. pacinii CGMCC 1.12557T and V. metschnikovii JCM 21189T was 20.6 ± 2.3, 38.1 ± 3.5 and 24.2 ± 2.8  %, respectively. The DNA G+C content was 46.8 mol% (T m). Based on the data, it is concluded that strain DSG-S6T represents a novel species of the genus Vibrio, for which the name Vibrio salilacus sp. nov. is proposed. The type strain is DSG-S6T ( = CGMCC 1.12427T = JCM 19265T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000316