RT Journal Article SR Electronic(1) A1 Kämpfer, Peter A1 Busse, Hans-Jürgen A1 McInroy, John A. A1 Xu, Jia A1 Glaeser, Stefanie P.YR 2015 T1 Flavobacterium nitrogenifigens sp. nov., isolated from switchgrass (Panicum virgatum) JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_9 SP 2803 OP 2809 DO https://doi.org/10.1099/ijs.0.000330 PB Microbiology Society, SN 1466-5034, AB A yellow, nitrogen-fixing bacterial strain, NXU-44T, isolated from the rhizosphere of switchgrass (Panicum virgatum) in Auburn, Alabama, USA, was studied to determine its taxonomic position. Cells of the isolate were rod-shaped and Gram-stain-negative. A comparison of the 16S rRNA gene sequence with the sequences of the type strains of the most closely related species showed that the strain belongs to the genus Flavobacterium with highest sequence similarities to the type strains of Flavobacterium ginsenosidimutans (97.9 %), Flavobacterium phragmitis (97.6 %) and Flavobacterium anhuiense (97.5 %). The 16S rRNA gene sequence similarities to all other species of the genus Flavobacterium were below 97.5 %. The fatty acid profile of strain NXU-44T consisted of the major fatty acids iso-C15 : 0, iso-C15 : 0 2-OH/C16 : 1ω7c and iso-C17 : 0 3-OH. The major compounds in the polar lipid profile were phosphatidylethanolamine, phosphatidylserine, one aminolipid and two polar lipids. The quinone system was composed exclusively of menaquinone MK-6. The polyamine pattern contained the major compound sym-homospermidine and only minor amounts of other polyamines. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. These data and the differential biochemical and chemotaxonomic properties show that strain NXU-44T represents a novel species of the genus Flavobacterium for which the name Flavobacterium nitrogenifigens sp. nov. is proposed. The type strain is NXU-44T ( = LMG 28694T = CIP 110894T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000330