@article{mbs:/content/journal/ijsem/10.1099/ijs.0.000338, author = "Zhong, Zhi-Ping and Liu, Ying and Liu, Hong-Can and Wang, Fang and Zhou, Yu-Guang and Liu, Zhi-Pei", title = "Marinobacter halophilus sp. nov., a halophilic bacterium isolated from a salt lake", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_9", pages = "2838-2845", doi = "https://doi.org/10.1099/ijs.0.000338", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000338", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-staining-negative bacterium, strain XCD-X12T, was isolated from Xiaochaidan Lake, a salt lake (salinity 9.9 %, w/w) in Qaidam basin, Qinghai Province, China. Its taxonomic position was determined by using a polyphasic approach. Cells of strain XCD-X12T were non-spore-forming rods, 0.4–0.7 μm wide, 2.1–3.2 μm long and motile with a single polar flagellum. Strain XCD-X12T was strictly aerobic and catalase- and oxidase-positive. Growth was observed in the presence of 0–20.0 % (w/v) NaCl (optimum, 4.0–8.0 %), at 4–35 °C (optimum, 30 °C) and at pH 6.5–10.5 (optimum, pH 8.5). It contained Q-9 as the predominant respiratory quinone. The major fatty acids (>10.0 %) were C16 : 0, C16 : 1ω9c and C18 : 1ω9c. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unknown phospholipids and an uncharacterized aminophospholipid. The DNA G+C content was 55.6 mol% (T m). Phylogenetic trees based on 16S rRNA gene sequences showed that strain XCD-X12T was associated with the genus Marinobacter, and showed the highest 16S rRNA gene sequence similarity to Marinobacter hydrocarbonoclasticus ATCC 49840T (97.4 %), M. vinifirmus FB1T (96.8 %), M. excellens KMM 3809T (96.8 %) and M. antarcticus ZS2-30T (96.7 %). DNA–DNA relatedness of strain XCD-X12T to M. hydrocarbonoclasticus CGMCC 1.7683T was 34 ± 5 %. Based on these data, it is concluded that strain XCD-X12T represents a novel species of the genus Marinobacter, for which the name Marinobacter halophilus sp. nov. is proposed. The type strain is XCD-X12T ( = CGMCC 1.12481T = JCM 30472T).", }