@article{mbs:/content/journal/ijsem/10.1099/ijs.0.000348, author = "Wang, Bing and Ji, Shi-Qi and Tian, Xin-Xin and Qu, Ling-Yun and Li, Fu-Li", title = "Brassicibacter thermophilus sp. nov., a thermophilic bacterium isolated from coastal sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_9", pages = "2870-2874", doi = "https://doi.org/10.1099/ijs.0.000348", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.000348", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel thermophilic, obligately anaerobic bacterium, strain Cel2fT, was isolated from a cellulolytic community enriched from coastal marine sediment. Cells were Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Optimal growth temperature and pH of strain Cel2fT were 55 °C and pH 7.0, respectively. NaCl was essential for the growth of strain Cel2fT and the strain showed enhanced growth in the presence of sea salt; the optimum sea salt concentration for growth was 7 % (w/v). Thiosulfate, sulfate and sulfite were potential electron acceptors. The major fatty acids of strain Cel2fT were iso-C15 : 0, C16 : 0, and C18 : 0. Polar lipid analysis indicated the presence of phosphatidylethanolamine and phosphatidylglycerol. Strain Cel2fT contained menaquinone MK-7 as the isoprenoid quinone, and the DNA G+C content was 31.3 mol%. Phylogenetic analysis revealed that the nearest relative of strain Cel2fT was Brassicibacter mesophilus BMT with 93.8 % 16S rRNA gene sequence similarity. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain Cel2fT represents a novel species of genus Brassicibacter, for which the name Brassicibacter thermophilus sp. nov. is proposed. The type strain is Cel2fT ( = JCM 30480T = CGMCC 1.5200T).", }