1887

Abstract

A Gram-stain-negative, yellow, rod-shaped bacterium, designated strain KK22, was isolated from a microbial consortium that grew on diesel fuel originally recovered from cattle pasture soil. Strain KK22 has been studied for its ability to biotransform high molecular weight polycyclic aromatic hydrocarbons. On the basis of 16S rRNA gene sequence phylogeny, strain KK22 was affiliated with the genus in the phylum and was most closely related to TKP (99.8  %) and less closely related to P25 (97.5  %). Results of DNA–DNA hybridization (DDH) revealed relatedness values between strain KK22 and strain TKP and between strain KK22 and strain P25 of 21 ± 4  % (reciprocal hybridization, 27 ± 2  %) and 15 ± 2  % (reciprocal hybridization, 17 ± 1  %), respectively. Chemotaxonomic analyses of strain KK22 showed that the major respiratory quinone was ubiquinone Q-10, that the polar lipid profile consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidyl--methylethylethanolamine and sphingoglycolipid, and that Cω7 and C 2-OH were the main fatty acid and hydroxylated fatty acids, respectively. This strain was unable to reduce nitrate and the genomic DNA G+C content was 64.7 mol%. Based upon the results of the DDH analyses, the fact that strain KK22 was motile, and its biochemical and physiological characteristics, strain KK22 could be separated from recognized species of the genus . We conclude that strain KK22 represents a novel species of this genus for which the name sp. nov. is proposed; the type strain is KK22 ( = DSM 29313 = JCM 30309).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000356
2015-09-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/9/2919.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000356&mimeType=html&fmt=ahah

References

  1. Arden Jones M.P., McCarthy A.J., Cross T. ( 1979;). Taxonomic and serologic studies on Micropolyspora faeni and Micropolyspora strains from soil bearing the specific epithet rectivirgula . J Gen Microbiol 115 343354 [View Article] [PubMed] .
    [Google Scholar]
  2. Atlas R.M. ( 1993). Handbook of Microbiological Media., Boca Raton, FL: CRC Press;.
    [Google Scholar]
  3. Bala K., Sharma P., Lal R. ( 2010;). Sphingobium quisquiliarum sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH-contaminated soil. Int J Syst Evol Microbiol 60 429433 [View Article] [PubMed] .
    [Google Scholar]
  4. Busse H.-J., Kämpfer P., Denner E.B.M. ( 1999;). Chemotaxonomic characterisation of Sphingomonas . J Ind Microbiol Biotechnol 23 242251 [View Article] [PubMed] .
    [Google Scholar]
  5. Cerniglia C.E. ( 1992;). Biodegradation of polycyclic aromatic hydrocarbons. Biodegradation 3 351368 [View Article].
    [Google Scholar]
  6. Dadhwal M., Jit S., Kumari H., Lal R. ( 2009;). Sphingobium chinhatense sp. nov., a hexachlorocyclohexane (HCH)-degrading bacterium isolated from an HCH dumpsite. Int J Syst Evol Microbiol 59 31403144 [View Article] [PubMed] .
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224229 [View Article].
    [Google Scholar]
  8. Gordon R.E., Mihm J.M. ( 1962;). Identification of Norcadia caviae (erikson) nov. comb.. Annals NY Acad Sci 98 628636. [CrossRef]
    [Google Scholar]
  9. Kanaly R.A., Bartha R. ( 1999;). Cometabolic mineralization of benzo[a]pyrene caused by hydrocarbon additions to soil. Environ Toxicol Chem 18 21862190. [CrossRef]
    [Google Scholar]
  10. Kanaly R.A., Harayama S. ( 2000;). Biodegradation of high-molecular-weight polycyclic aromatic hydrocarbons by bacteria. J Bacteriol 182 20592067 [View Article] [PubMed] .
    [Google Scholar]
  11. Kanaly R.A., Harayama S. ( 2010;). Advances in the field of high-molecular-weight polycyclic aromatic hydrocarbon biodegradation by bacteria. Microb Biotechnol 3 136164 [View Article] [PubMed] .
    [Google Scholar]
  12. Kanaly R., Bartha R., Fogel S., Findlay M. ( 1997;). Biodegradation of [(sup14)C]benzo[a]pyrene added in crude oil to uncontaminated soil. Appl Environ Microbiol 63 45114515 [PubMed].
    [Google Scholar]
  13. Kanaly R.A., Bartha R., Watanabe K., Harayama S. ( 2000;). Rapid mineralization of benzo[a]pyrene by a microbial consortium growing on diesel fuel. Appl Environ Microbiol 66 42054211 [View Article] [PubMed] .
    [Google Scholar]
  14. Kunihiro M., Ozeki Y., Nogi Y., Hamamura N., Kanaly R.A. ( 2013;). Benz[a]anthracene biotransformation and production of ring fission products by Sphingobium sp. strain KK22. Appl Environ Microbiol 79 44104420 [View Article] [PubMed] .
    [Google Scholar]
  15. Maeda A.H., Nishi S., Ozeki Y., Ohta Y., Hatada Y., Kanaly R.A. ( 2013;). Draft genome sequence of Sphingobium sp. strain KK22, a high-molecular-weight polycyclic aromatic hydrocarbon-degrading bacterium isolated from cattle pasture soil. Genome Announc 1 e00911e00913 [View Article] [PubMed] .
    [Google Scholar]
  16. Maeda A.H., Nishi S., Hatada Y., Ozeki Y., Kanaly R.A. ( 2014;). Biotransformation of the high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by Sphingobium sp. strain KK22 and identification of new products of non-alternant PAH biodegradation by liquid chromatography electrospray ionization tandem mass spectrometry. Microb Biotechnol 7 114129 [View Article] [PubMed] .
    [Google Scholar]
  17. Minnikin D.E., Collins M.D., Goodfellow M. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47 8795 [View Article].
    [Google Scholar]
  18. Nishijima M., Araki-Sakai M., Sano H. ( 1997;). Identification of isoprenoid quinones by frit-FAB liquid chromatography-mass spectrometry for the chemotaxonomy of microorganisms. J Microbiol Methods 28 113122 [View Article].
    [Google Scholar]
  19. Prakash O., Lal R. ( 2006;). Description of Sphingobium fuliginis sp. nov., a phenanthrene-degrading bacterium from a fly ash dumping site, and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov. Int J Syst Evol Microbiol 56 21472152 [View Article] [PubMed] .
    [Google Scholar]
  20. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  21. Smibert R.M., Krieg N.R. ( 1994;). Pehnotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607655. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  22. Stackebrandt E. ( 2011;). Molecular taxonomic parameters. Microbiol Aust 32 5961.
    [Google Scholar]
  23. Stackebrandt E., Ebers J. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33 152155.
    [Google Scholar]
  24. Stackebrandt E., Goebel B.M. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 44 846849.
    [Google Scholar]
  25. Stolz A. ( 2009;). Molecular characteristics of xenobiotic-degrading sphingomonads. Appl Microbiol Biotechnol 81 793811 [View Article] [PubMed] .
    [Google Scholar]
  26. Takeuchi M., Hamana K., Hiraishi A. ( 2001;). Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51 14051417 [PubMed]. [CrossRef]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed] .
    [Google Scholar]
  28. Thompson J.D., Higgins D.G., Gibson T.J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 46734680 [View Article] [PubMed] .
    [Google Scholar]
  29. Tindall B.J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60 249266 [View Article] [PubMed] .
    [Google Scholar]
  30. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Evol Microbiol 37 463464.
    [Google Scholar]
  31. Young C.C., Ho M.-J., Arun A.B., Chen W.-M., Lai W.-A., Shen F.-T., Rekha P.D., Yassin A.F. ( 2007;). Sphingobium olei sp. nov., isolated from oil-contaminated soil. Int J Syst Evol Microbiol 57 26132617 [View Article] [PubMed] .
    [Google Scholar]
  32. Young C.C., Arun A.B., Kämpfer P., Busse H.-J., Lai W.-A., Chen W.-M., Shen F.-T., Rekha P.D. ( 2008;). Sphingobium rhizovicinum sp. nov., isolated from rhizosphere soil of Fortunella hindsii (Champ. ex Benth.) Swingle. Int J Syst Evol Microbiol 58 18011806 [View Article] [PubMed] .
    [Google Scholar]
  33. Zipper C., Nickel K., Angst W., Kohler H.-P.E. ( 1996;). Complete microbial degradation of both enantiomers of the chiral herbicide mecoprop [(RS)-2-(4-chloro-2-methylphenoxy)propionic acid] in an enantioselective manner by Sphingomonas herbicidovorans sp. nov. Appl Environ Microbiol 62 43184322 [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000356
Loading
/content/journal/ijsem/10.1099/ijs.0.000356
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error